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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi130 – 1301Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi133 – 1331Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi172 – 1721Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi175 – 1751Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi179 – 1791Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-28.5kD
Short name:
CI-28.5kD
NADH-ubiquinone oxidoreductase 28.5 kDa subunit
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141Mitochondrion1 PublicationAdd
BLAST
Chaini42 – 229188NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020017Add
BLAST

Expressioni

Tissue specificityi

Lowest expression found in storage tissues of tubers. Higher expression in older leaves than younger ones. Highest expression found in flowers.

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400084285.

Structurei

3D structure databases

ProteinModelPortaliP80269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 150304Fe-4S ferredoxin-type 1Add
BLAST
Domaini160 – 189304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

InParanoidiP80269.
KOiK03941.
OMAiFEFATEM.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAILARKSL SALRSRQLVL AGHTIEGTNG YNRTLLGTRS FATKHSFSTD
60 70 80 90 100
KDDEEREQLA KELSKDWNSV FERSINTLFL TEMVRGLMLT LKYFFEKKVT
110 120 130 140 150
INYPFEKGPL SPRFRGEHAL RRYATGEERC IACKLCEAIC PAQAITIEAE
160 170 180 190 200
EREDGSRRTT RYDIDMTKCI YCGFCQEACP VDAIVEGPNF EFATETHEEL
210 220
LYDKEKLLEN GDRWETEIAE NLRSESLYR
Length:229
Mass (Da):26,378
Last modified:May 30, 2000 - v2
Checksum:iD995415DBE064A93
GO

Polymorphismi

There are two alleles; TYKY1 (shown here) and TYKY2.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 264HTIE → QAWQ in allele TYKY2.
Natural varianti30 – 334GYNR → TPNG in allele TYKY2.
Natural varianti57 – 571E → R in strain: cv. Bintje.
Natural varianti62 – 621E → K in strain: cv. Bintje.
Natural varianti65 – 651K → T in strain: cv. Bintje.
Natural varianti121 – 1211R → H in allele TYKY2.
Natural varianti216 – 2161T → I in allele TYKY2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84319 mRNA. Translation: CAA59062.1.
X84320 mRNA. Translation: CAA59063.1.
PIRiS52385.
S52386.
RefSeqiNP_001275214.1. NM_001288285.1.
UniGeneiStu.18061.
Stu.18573.

Genome annotation databases

EnsemblPlantsiPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912.
GeneIDi102589342.
KEGGisot:102589342.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84319 mRNA. Translation: CAA59062.1.
X84320 mRNA. Translation: CAA59063.1.
PIRiS52385.
S52386.
RefSeqiNP_001275214.1. NM_001288285.1.
UniGeneiStu.18061.
Stu.18573.

3D structure databases

ProteinModelPortaliP80269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400084285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912.
GeneIDi102589342.
KEGGisot:102589342.

Phylogenomic databases

InParanoidiP80269.
KOiK03941.
OMAiFEFATEM.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants."
    Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L.
    Mol. Gen. Genet. 253:448-454(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES TYKY1 AND TYKY2).
    Strain: cv. Desiree.
    Tissue: Leaf.
  2. "Genome sequence and analysis of the tuber crop potato."
    The Potato Genome Sequencing Consortium
    Nature 475:189-195(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. DM1-3 516 R44.
  3. "Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum."
    Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L.
    J. Biol. Chem. 269:2263-2269(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 42-65.
    Strain: cv. Bintje.
    Tissue: Tuber.

Entry informationi

Entry nameiNDUS8_SOLTU
AccessioniPrimary (citable) accession number: P80269
Secondary accession number(s): Q43849, Q43850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: June 24, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally reported that TYKY1 and TYKY2 were two different genes.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.