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P80269

- NDUS8_SOLTU

UniProt

P80269 - NDUS8_SOLTU

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. May donate electrons to ubiquinone.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi130 – 1301Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi133 – 1331Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi172 – 1721Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi175 – 1751Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi179 – 1791Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-28.5kD
    Short name:
    CI-28.5kD
    NADH-ubiquinone oxidoreductase 28.5 kDa subunit
    OrganismiSolanum tuberosum (Potato)
    Taxonomic identifieri4113 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
    ProteomesiUP000011115: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4141Mitochondrion1 PublicationAdd
    BLAST
    Chaini42 – 229188NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020017Add
    BLAST

    Expressioni

    Tissue specificityi

    Lowest expression found in storage tissues of tubers. Higher expression in older leaves than younger ones. Highest expression found in flowers.

    Interactioni

    Subunit structurei

    Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP80269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini121 – 150304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini160 – 189304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    KOiK03941.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF12838. Fer4_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80269-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAILARKSL SALRSRQLVL AGHTIEGTNG YNRTLLGTRS FATKHSFSTD    50
    KDDEEREQLA KELSKDWNSV FERSINTLFL TEMVRGLMLT LKYFFEKKVT 100
    INYPFEKGPL SPRFRGEHAL RRYATGEERC IACKLCEAIC PAQAITIEAE 150
    EREDGSRRTT RYDIDMTKCI YCGFCQEACP VDAIVEGPNF EFATETHEEL 200
    LYDKEKLLEN GDRWETEIAE NLRSESLYR 229
    Length:229
    Mass (Da):26,378
    Last modified:May 30, 2000 - v2
    Checksum:iD995415DBE064A93
    GO

    Polymorphismi

    There are two alleles; TYKY1 (shown here) and TYKY2.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti23 – 264HTIE → QAWQ in allele TYKY2.
    Natural varianti30 – 334GYNR → TPNG in allele TYKY2.
    Natural varianti57 – 571E → R in strain: cv. Bintje.
    Natural varianti62 – 621E → K in strain: cv. Bintje.
    Natural varianti65 – 651K → T in strain: cv. Bintje.
    Natural varianti121 – 1211R → H in allele TYKY2.
    Natural varianti216 – 2161T → I in allele TYKY2.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84319 mRNA. Translation: CAA59062.1.
    X84320 mRNA. Translation: CAA59063.1.
    PIRiS52385.
    S52386.
    RefSeqiNP_001275214.1. NM_001288285.1.
    UniGeneiStu.18061.
    Stu.18573.

    Genome annotation databases

    EnsemblPlantsiPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912.
    GeneIDi102589342.
    KEGGisot:102589342.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84319 mRNA. Translation: CAA59062.1 .
    X84320 mRNA. Translation: CAA59063.1 .
    PIRi S52385.
    S52386.
    RefSeqi NP_001275214.1. NM_001288285.1.
    UniGenei Stu.18061.
    Stu.18573.

    3D structure databases

    ProteinModelPortali P80269.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi PGSC0003DMT400084285 ; PGSC0003DMT400084285 ; PGSC0003DMG400033912 .
    GeneIDi 102589342.
    KEGGi sot:102589342.

    Phylogenomic databases

    KOi K03941.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF12838. Fer4_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants."
      Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L.
      Mol. Gen. Genet. 253:448-454(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES TYKY1 AND TYKY2).
      Strain: cv. Desiree.
      Tissue: Leaf.
    2. "Genome sequence and analysis of the tuber crop potato."
      The Potato Genome Sequencing Consortium
      Nature 475:189-195(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. DM1-3 516 R44.
    3. "Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum."
      Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L.
      J. Biol. Chem. 269:2263-2269(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 42-65.
      Strain: cv. Bintje.
      Tissue: Tuber.

    Entry informationi

    Entry nameiNDUS8_SOLTU
    AccessioniPrimary (citable) accession number: P80269
    Secondary accession number(s): Q43849, Q43850
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally reported that TYKY1 and TYKY2 were two different genes.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3