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P80269

- NDUS8_SOLTU

UniProt

P80269 - NDUS8_SOLTU

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Protein
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. May donate electrons to ubiquinone.UniRule annotation

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).UniRule annotation
NADH + acceptor = NAD+ + reduced acceptor.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi130 – 1301Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi133 – 1331Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi136 – 1361Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi169 – 1691Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi172 – 1721Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi175 – 1751Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi179 – 1791Iron-sulfur 1 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-28.5kD
Short name:
CI-28.5kD
NADH-ubiquinone oxidoreductase 28.5 kDa subunit
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141Mitochondrion1 Publication
Add
BLAST
Chaini42 – 229188NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialUniRule annotation
PRO_0000020017Add
BLAST

Expressioni

Tissue specificityi

Lowest expression found in storage tissues of tubers. Higher expression in older leaves than younger ones. Highest expression found in flowers.

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Structurei

3D structure databases

ProteinModelPortaliP80269.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 150304Fe-4S ferredoxin-type 1
Add
BLAST
Domaini160 – 189304Fe-4S ferredoxin-type 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

KOiK03941.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80269-1 [UniParc]FASTAAdd to Basket

« Hide

MAAILARKSL SALRSRQLVL AGHTIEGTNG YNRTLLGTRS FATKHSFSTD    50
KDDEEREQLA KELSKDWNSV FERSINTLFL TEMVRGLMLT LKYFFEKKVT 100
INYPFEKGPL SPRFRGEHAL RRYATGEERC IACKLCEAIC PAQAITIEAE 150
EREDGSRRTT RYDIDMTKCI YCGFCQEACP VDAIVEGPNF EFATETHEEL 200
LYDKEKLLEN GDRWETEIAE NLRSESLYR 229
Length:229
Mass (Da):26,378
Last modified:May 30, 2000 - v2
Checksum:iD995415DBE064A93
GO

Polymorphismi

There are two alleles; TYKY1 (shown here) and TYKY2.UniRule annotation

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 264HTIE → QAWQ in allele TYKY2.
Natural varianti30 – 334GYNR → TPNG in allele TYKY2.
Natural varianti57 – 571E → R in strain: cv. Bintje.
Natural varianti62 – 621E → K in strain: cv. Bintje.
Natural varianti65 – 651K → T in strain: cv. Bintje.
Natural varianti121 – 1211R → H in allele TYKY2.
Natural varianti216 – 2161T → I in allele TYKY2.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84319 mRNA. Translation: CAA59062.1.
X84320 mRNA. Translation: CAA59063.1.
PIRiS52385.
S52386.
RefSeqiNP_001275214.1. NM_001288285.1.
UniGeneiStu.18061.
Stu.18573.

Genome annotation databases

EnsemblPlantsiPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912.
GeneIDi102589342.
KEGGisot:102589342.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84319 mRNA. Translation: CAA59062.1 .
X84320 mRNA. Translation: CAA59063.1 .
PIRi S52385.
S52386.
RefSeqi NP_001275214.1. NM_001288285.1.
UniGenei Stu.18061.
Stu.18573.

3D structure databases

ProteinModelPortali P80269.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi PGSC0003DMT400084285 ; PGSC0003DMT400084285 ; PGSC0003DMG400033912 .
GeneIDi 102589342.
KEGGi sot:102589342.

Phylogenomic databases

KOi K03941.

Family and domain databases

HAMAPi MF_01351. NDH1_NuoI.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view ]
Pfami PF12838. Fer4_7. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01971. NuoI. 1 hit.
PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants."
    Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L.
    Mol. Gen. Genet. 253:448-454(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES TYKY1 AND TYKY2).
    Strain: cv. Desiree.
    Tissue: Leaf.
  2. "Genome sequence and analysis of the tuber crop potato."
    The Potato Genome Sequencing Consortium
    Nature 475:189-195(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. DM1-3 516 R44.
  3. "Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum."
    Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L.
    J. Biol. Chem. 269:2263-2269(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 42-65.
    Strain: cv. Bintje.
    Tissue: Tuber.

Entry informationi

Entry nameiNDUS8_SOLTU
AccessioniPrimary (citable) accession number: P80269
Secondary accession number(s): Q43849, Q43850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) reported that TYKY1 and TYKY2 were two different genes.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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