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P80269 (NDUS8_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-28.5kD
Short name=CI-28.5kD
NADH-ubiquinone oxidoreductase 28.5 kDa subunit
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. May donate electrons to ubiquinone. HAMAP-Rule MF_01351

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01351

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP-Rule MF_01351

Subunit structure

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side HAMAP-Rule MF_01351.

Tissue specificity

Lowest expression found in storage tissues of tubers. Higher expression in older leaves than younger ones. Highest expression found in flowers.

Polymorphism

There are two alleles; TYKY1 (shown here) and TYKY2. HAMAP-Rule MF_01351

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Caution

Was originally (Ref.1) reported that TYKY1 and TYKY2 were two different genes.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4141Mitochondrion Ref.3
Chain42 – 229188NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial HAMAP-Rule MF_01351
PRO_0000020017

Regions

Domain121 – 150304Fe-4S ferredoxin-type 1
Domain160 – 189304Fe-4S ferredoxin-type 2

Sites

Metal binding1301Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1331Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1361Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1401Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1691Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1721Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1751Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1791Iron-sulfur 1 (4Fe-4S) By similarity

Natural variations

Natural variant23 – 264HTIE → QAWQ in allele TYKY2.
Natural variant30 – 334GYNR → TPNG in allele TYKY2.
Natural variant571E → R in strain: cv. Bintje.
Natural variant621E → K in strain: cv. Bintje.
Natural variant651K → T in strain: cv. Bintje.
Natural variant1211R → H in allele TYKY2.
Natural variant2161T → I in allele TYKY2.

Sequences

Sequence LengthMass (Da)Tools
P80269 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: D995415DBE064A93

FASTA22926,378
        10         20         30         40         50         60 
MAAILARKSL SALRSRQLVL AGHTIEGTNG YNRTLLGTRS FATKHSFSTD KDDEEREQLA 

        70         80         90        100        110        120 
KELSKDWNSV FERSINTLFL TEMVRGLMLT LKYFFEKKVT INYPFEKGPL SPRFRGEHAL 

       130        140        150        160        170        180 
RRYATGEERC IACKLCEAIC PAQAITIEAE EREDGSRRTT RYDIDMTKCI YCGFCQEACP 

       190        200        210        220 
VDAIVEGPNF EFATETHEEL LYDKEKLLEN GDRWETEIAE NLRSESLYR 

« Hide

References

« Hide 'large scale' references
[1]"The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants."
Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L.
Mol. Gen. Genet. 253:448-454(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES TYKY1 AND TYKY2).
Strain: cv. Desiree.
Tissue: Leaf.
[2]"Genome sequence and analysis of the tuber crop potato."
The Potato Genome Sequencing Consortium
Nature 475:189-195(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. DM1-3 516 R44.
[3]"Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum."
Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L.
J. Biol. Chem. 269:2263-2269(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 42-65.
Strain: cv. Bintje.
Tissue: Tuber.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84319 mRNA. Translation: CAA59062.1.
X84320 mRNA. Translation: CAA59063.1.
PIRS52385.
S52386.
RefSeqNP_001275214.1. NM_001288285.1.
UniGeneStu.18061.
Stu.18573.

3D structure databases

ProteinModelPortalP80269.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912.
GeneID102589342.

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS8_SOLTU
AccessionPrimary (citable) accession number: P80269
Secondary accession number(s): Q43849, Q43850
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: March 19, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families