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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene
N/A
Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Caution

Was originally reported that TYKY1 and TYKY2 were two different genes.1 Publication

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi130Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi133Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi136Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi140Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi169Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi172Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi175Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi179Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-28.5kD
Short name:
CI-28.5kD
NADH-ubiquinone oxidoreductase 28.5 kDa subunit
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41Mitochondrion1 PublicationAdd BLAST41
ChainiPRO_000002001742 – 229NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialAdd BLAST188

Expressioni

Tissue specificityi

Lowest expression found in storage tissues of tubers. Higher expression in older leaves than younger ones. Highest expression found in flowers.

Gene expression databases

ExpressionAtlasiP80269 baseline

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

STRINGi4113.PGSC0003DMT400084285

Structurei

3D structure databases

ProteinModelPortaliP80269
SMRiP80269
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini121 – 1504Fe-4S ferredoxin-type 1Add BLAST30
Domaini160 – 1894Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256 Eukaryota
COG1143 LUCA
InParanoidiP80269
KOiK03941
OMAiVACYMCQ
OrthoDBiEOG09360O5Z

Family and domain databases

HAMAPiMF_01351 NDH1_NuoI, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR010226 NADH_quinone_OxRdtase_chainI
PfamiView protein in Pfam
PF12838 Fer4_7, 1 hit
TIGRFAMsiTIGR01971 NuoI, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAILARKSL SALRSRQLVL AGHTIEGTNG YNRTLLGTRS FATKHSFSTD
60 70 80 90 100
KDDEEREQLA KELSKDWNSV FERSINTLFL TEMVRGLMLT LKYFFEKKVT
110 120 130 140 150
INYPFEKGPL SPRFRGEHAL RRYATGEERC IACKLCEAIC PAQAITIEAE
160 170 180 190 200
EREDGSRRTT RYDIDMTKCI YCGFCQEACP VDAIVEGPNF EFATETHEEL
210 220
LYDKEKLLEN GDRWETEIAE NLRSESLYR
Length:229
Mass (Da):26,378
Last modified:May 30, 2000 - v2
Checksum:iD995415DBE064A93
GO

Polymorphismi

There are two alleles; TYKY1 (shown here) and TYKY2.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti23 – 26HTIE → QAWQ in allele TYKY2. 4
Natural varianti30 – 33GYNR → TPNG in allele TYKY2. 4
Natural varianti57E → R in strain: cv. Bintje. 1
Natural varianti62E → K in strain: cv. Bintje. 1
Natural varianti65K → T in strain: cv. Bintje. 1
Natural varianti121R → H in allele TYKY2. 1
Natural varianti216T → I in allele TYKY2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84319 mRNA Translation: CAA59062.1
X84320 mRNA Translation: CAA59063.1
PIRiS52385
S52386
RefSeqiNP_001275214.1, NM_001288285.1
UniGeneiStu.18061
Stu.18573

Genome annotation databases

EnsemblPlantsiPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912
GeneIDi102589342
GrameneiPGSC0003DMT400084285; PGSC0003DMT400084285; PGSC0003DMG400033912
KEGGisot:102589342

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNDUS8_SOLTU
AccessioniPrimary (citable) accession number: P80269
Secondary accession number(s): Q43849, Q43850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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