Reviewed, UniProtKB/Swiss-Prot P80264 (NDUS2_SOLTU)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 49 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH dehydrogenase subunit 7
    Complex I-42.5 kDa
      Short name=CI-42.5kD

Gene names

Name:

NAD7

Encoded on

Mitochondrion

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	47 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Subunit structure	Complex I is composed of about 30 different subunits. This is a component of the iron-sulfur protein fraction.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.
RNA editing	Edited at positions 13, 26 and 28.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – ›47	›46	NADH-ubiquinone oxidoreductase 49 kDa subunit		PRO_0000118586
Experimental info
	Non-terminal residue	47	1

Sequences

	Sequence		Length	Mass (Da)
	P80264-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0494931B92D06A87	FASTA	47	5,278
	10 20 30 40 MTTKNRQIQN FTLNFGPQHP AAHGVLRLVL EMNGEVVERA EPHIGLL

References

[1]	"The 42.5 kDa subunit of the NADH: ubiquinone oxidoreductase (complex I) in higher plants is encoded by the mitochondrial nad7 gene." Gabler L., Herz U., Liddell A., Leaver C.J., Schroeder W., Brennicke A., Grohmann L. Mol. Gen. Genet. 244:33-40(1994) [PubMed: 8041359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING. Tissue: Tuber.
[2]	"Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum." Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L. J. Biol. Chem. 269:2263-2269(1994) [PubMed: 8294484] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-37. Strain: cv. Bintje. Tissue: Tuber.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75579 Genomic DNA. Translation: CAA53256.1. Sequence problems.
PIR	S38586. S46439.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014029. NADH-UbQ_OxRdtase_49kDa_CS. [Graphical view]
PROSITE	PS00535. COMPLEX1_49K. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_SOLTU

Accession

Primary (citable) accession number: P80264
Secondary accession number(s): Q43185

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)