Reviewed, UniProtKB/Swiss-Prot P80261 (NDUS3_SOLTU)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 27 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-27 kDa
      Short name=CI-27kD
    NADH dehydrogenase subunit 9

Gene names

Name:

NAD9

Encoded on

Mitochondrion

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Subunit structure	Complex I is composed of about 30 different subunits.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.
RNA editing	Edited at positions 31, 56, 100, 110, 122, 133 and 147.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	NADH-ubiquinone oxidoreductase 27 kDa subunit		PRO_0000118643

Sequences

	Sequence		Length	Mass (Da)
	P80261-1 [UniParc]. Last modified November 28, 2003. Version 3. Checksum: A150CF461B493A53	FASTA	190	22,883
	10 20 30 40 50 60 MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTNTDYLFQ LLCFMKLHTY TRVQVLIDIC 70 80 90 100 110 120 GVDYPSRKQR FEVVYNLLSI RYNSRIRVQT SADEVTRISS VVSLFPSAGW WEREVWDMFG 130 140 150 160 170 180 VFSINHPDLR RILTDYGFEG HPLRKDFPLS GYVEVRYDDP EKRVVSEPIE MTQEFRYFDF 190 ASPWEQRSDG

References

[1]	"Translation of nad9 mRNAs in mitochondria from Solanum tuberosum is restricted to completely edited transcripts." Grohmann L., Thieck O., Herz U., Schroeder W., Brennicke A. Nucleic Acids Res. 22:3304-3311(1994) [PubMed: 8078764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, RNA EDITING. Tissue: Tuber.
[2]	"Purification of the NADH:ubiquinone oxidoreductase (complex I) of the respiratory chain from the inner mitochondrial membrane of Solanum tuberosum." Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L. J. Biol. Chem. 269:2263-2269(1994) [PubMed: 8294484] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-17. Strain: cv. Bintje. Tissue: Tuber.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79774 Genomic DNA. Translation: CAA56168.1. Sequence problems.
PIR	S48062.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_DHase_Ub_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_SOLTU

Accession

Primary (citable) accession number: P80261

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 28, 2003
Last modified:	November 25, 2008
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)