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P80235

- CACM_YEAST

UniProt

P80235 - CACM_YEAST

Protein

Putative mitochondrial carnitine O-acetyltransferase

Gene

YAT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Involved in the transfer of acetyl-CoA into mitochondria. May also be involved in the metabolism of acetate and of ethanol.

    Catalytic activityi

    Acetyl-CoA + carnitine = CoA + O-acetylcarnitine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei346 – 3461Proton acceptorBy similarity
    Binding sitei481 – 4811CarnitineBy similarity
    Binding sitei494 – 4941CarnitineBy similarity

    GO - Molecular functioni

    1. carnitine O-acetyltransferase activity Source: SGD

    GO - Biological processi

    1. alcohol metabolic process Source: SGD
    2. carnitine metabolic process Source: SGD
    3. fatty acid metabolic process Source: UniProtKB-KW
    4. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism, Transport

    Enzyme and pathway databases

    BioCyciYEAST:YAR035W-MONOMER.
    ReactomeiREACT_188984. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative mitochondrial carnitine O-acetyltransferase (EC:2.3.1.7)
    Gene namesi
    Name:YAT1
    Ordered Locus Names:YAR035W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAR035w.
    SGDiS000000080. YAT1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 687687Putative mitochondrial carnitine O-acetyltransferasePRO_0000210175Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei517 – 5171Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP80235.
    PeptideAtlasiP80235.

    Expressioni

    Inductioni

    By ethanol and by acetate. Repressed by glucose, and to a lesser extent, by galactose. Derepressed by glycerol.

    Gene expression databases

    GenevestigatoriP80235.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACS1Q015741EBI-3928,EBI-2128

    Protein-protein interaction databases

    BioGridi31811. 14 interactions.
    DIPiDIP-3795N.
    IntActiP80235. 9 interactions.
    MINTiMINT-541328.
    STRINGi4932.YAR035W.

    Structurei

    3D structure databases

    ProteinModelPortaliP80235.
    SMRiP80235. Positions 3-655.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni446 – 45914Coenzyme A bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG70127.
    GeneTreeiENSGT00550000074786.
    HOGENOMiHOG000206461.
    KOiK00624.
    OMAiENTYEPA.
    OrthoDBiEOG7T7H2J.

    Family and domain databases

    InterProiIPR000542. Carn_acyl_trans.
    [Graphical view]
    PANTHERiPTHR22589. PTHR22589. 1 hit.
    PfamiPF00755. Carn_acyltransf. 1 hit.
    [Graphical view]
    PROSITEiPS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P80235-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPNLKRLPIP PLQDTLNRYL ARVEPLQDER QNRRTRRTVL SAENLDALNT    50
    LHERLLEYDA RLAESNPESS YIEQFWYDAY LLYDATVVLN VNPYFQLQDD 100
    PTIKDTPETA AQGPYGAHTV QVRRAARLTT SILKFIRQIR HGTLRTDTVR 150
    GKTPLSMDQY ERLFGSSRIP PGPGEPSCHL QTDATSHHVV AMYRGQFYWF 200
    DVLDTRNEPI FATPEQLEWN LYSIIMDAES AGSGSAPFGV FTTESRRVWS 250
    NIRDYLFHAD DCTNWRNLKL IDSALFVVCL DDVAFAADQQ DELTRSMLCG 300
    TSTINLDPHQ HQPPLNVQTG TCLNRWYDKL QLIVTKNGKA GINFEHTGVD 350
    GHTVLRLATD IYTDSILSFA RGVTKNVVDI FSDDDGKPSS SSLASAAHSA 400
    NLITIPRKLE WRTDNFLQSS LHFAETRISD LISQYEFVNL DFSNYGASHI 450
    KTVFKCSPDA FVQQVFQVAY FALYGRFETV YEPAMTKAFQ NGRTEAIRSV 500
    TGQSKLFVKS LLDQDASDAT KIQLLHDACT AHSQITRECS QGLGQDRHLY 550
    ALYCLWNQWY KDKLELPPIF RDKSWTTMQN NVLSTSNCGN PCLKSFGFGP 600
    VTANGFGIGY IIRDHSVSVV VSSRHRQTAR FASLMEKSLL EIDRIFKRQQ 650
    ARAAKPAART TASANTKSED MKYLLSGYDY FDVSVSG 687
    Length:687
    Mass (Da):77,766
    Last modified:December 6, 2005 - v2
    Checksum:iF00B628893B8BB39
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251P → T in CAA52647. (PubMed:8262985)Curated
    Sequence conflicti392 – 3921S → SS in CAA52647. (PubMed:8262985)Curated
    Sequence conflicti595 – 5962SF → AS in CAA52647. (PubMed:8262985)Curated
    Sequence conflicti653 – 6531A → T in CAA52647. (PubMed:8262985)Curated
    Sequence conflicti660 – 6601T → A in CAA52647. (PubMed:8262985)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74553 Genomic DNA. Translation: CAA52647.1.
    L28920 Genomic DNA. Translation: AAC09495.1.
    BK006935 Genomic DNA. Translation: DAA07004.1.
    PIRiS53485.
    RefSeqiNP_009420.1. NM_001178226.1.

    Genome annotation databases

    EnsemblFungiiYAR035W; YAR035W; YAR035W.
    GeneIDi851285.
    KEGGisce:YAR035W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74553 Genomic DNA. Translation: CAA52647.1 .
    L28920 Genomic DNA. Translation: AAC09495.1 .
    BK006935 Genomic DNA. Translation: DAA07004.1 .
    PIRi S53485.
    RefSeqi NP_009420.1. NM_001178226.1.

    3D structure databases

    ProteinModelPortali P80235.
    SMRi P80235. Positions 3-655.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31811. 14 interactions.
    DIPi DIP-3795N.
    IntActi P80235. 9 interactions.
    MINTi MINT-541328.
    STRINGi 4932.YAR035W.

    Proteomic databases

    PaxDbi P80235.
    PeptideAtlasi P80235.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAR035W ; YAR035W ; YAR035W .
    GeneIDi 851285.
    KEGGi sce:YAR035W.

    Organism-specific databases

    CYGDi YAR035w.
    SGDi S000000080. YAT1.

    Phylogenomic databases

    eggNOGi NOG70127.
    GeneTreei ENSGT00550000074786.
    HOGENOMi HOG000206461.
    KOi K00624.
    OMAi ENTYEPA.
    OrthoDBi EOG7T7H2J.

    Enzyme and pathway databases

    BioCyci YEAST:YAR035W-MONOMER.
    Reactomei REACT_188984. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 968285.
    PROi P80235.

    Gene expression databases

    Genevestigatori P80235.

    Family and domain databases

    InterProi IPR000542. Carn_acyl_trans.
    [Graphical view ]
    PANTHERi PTHR22589. PTHR22589. 1 hit.
    Pfami PF00755. Carn_acyltransf. 1 hit.
    [Graphical view ]
    PROSITEi PS00439. ACYLTRANSF_C_1. 1 hit.
    PS00440. ACYLTRANSF_C_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ethanol-inducible YAT1 gene from yeast encodes a presumptive mitochondrial outer carnitine acetyltransferase."
      Schmalix W.A., Bandlow W.
      J. Biol. Chem. 268:27428-27439(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DL-1.
    2. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52 Kbp CDC15-FLO1-PHO11-YAR074 region."
      Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B., Kaback D.B., Clark M.W.
      Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
      Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
      Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiCACM_YEAST
    AccessioniPrimary (citable) accession number: P80235
    Secondary accession number(s): D6VPN4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3