Reviewed, UniProtKB/Swiss-Prot P80227 (ACPH_BOVIN)

Names and origin

Protein names

Recommended name:
    Acylamino-acid-releasing enzyme
      Short name=AARE
    EC=3.4.19.1
Alternative name(s):
    Acyl-peptide hydrolase
      Short name=APH
    Acylaminoacyl-peptidase

Gene names

Name:

APEH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
Catalytic activity	Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S9C family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Acylamino-acid-releasing enzyme		PRO_0000122429
Sites
	Active site	585	1	Charge relay system By similarity
	Active site	673	1	Charge relay system By similarity
	Active site	705	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80227-1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 838882B5B703DF15	FASTA	730	81,093
	10 20 30 40 50 60 MERQVLLSEP EEAAALYRGL SRQPALSAAC LGPEVTTQYG GRYRTVHTEW TQRDLERMEN 70 80 90 100 110 120 IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG TMKAVLRKAG STGEEKQFLE 130 140 150 160 170 180 VWEKNRKLKS FNLSALEKHG PVYEDDCFGC LSWSHSETHL LYVAEKKRPK AESFFQTKAL 190 200 210 220 230 240 DISGSDDEMA RPKKPDQAIK GDQFLFYEDW GENMVSKGSP VLCVLDIESG NISVLEGVPE 250 260 270 280 290 300 SVSPGQAFWA PGDTGVVFAG WWHEPFRLGI RFCTNRRSAL YYVDLTGGNC ELLSDDSLAV 310 320 330 340 350 360 TSPRLSPDQC RIVYLQFPSL VPHQQCGQLC LYDWYTRVTV VVVDVVPRQL GENFSGIYCS 370 380 390 400 410 420 LLPLGCWSAD SQRVVFDTAQ RSRQDLFAVD TQMGTVTPLT AGGSGGSWKL LTIDRDLMVA 430 440 450 460 470 480 QFSTPNLPPC LKVGFLPPAG MEQEVVWVSL EEAEPIPDIS WSIRVLQPPP EQEHAQYVGL 490 500 510 520 530 540 DFEAILIQPS NPPDKTQVPM VVMPHGGPHS SFVTSWMLLP AMLCKMGFAA LLVNYRGSTG 550 560 570 580 590 600 FGQDSILSLP GNVGSQDVKD VQFAVEQVLQ EEHFDAGRVA LLGGSHGGFL SCHLIGQYPE 610 620 630 640 650 660 TYGACVVRNP VINIASMMGS TDIPDWCVVE AGYLYSSDCL PDPNVWSEML NKSPIKYTPQ 670 680 690 700 710 720 VKTPVLLMLG QEDRRVPFKQ GMEYYRALKA RNVPVRLLLY PKSTHSLSEV EVESDSFMNA 730 VIWMCTHLGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thalamus.
[2]	"Bovine lens acylpeptide hydrolase. Purification and characterization of a tetrameric enzyme resistant to urea denaturation and proteolytic inactivation." Sharma K.K., Ortwerth B.J. Eur. J. Biochem. 216:631-637(1993) [PubMed: 8375399] [Abstract] Cited for: PROTEIN SEQUENCE OF 194-213. Tissue: Lens.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC123400 mRNA. Translation: AAI23401.1.
IPI	IPI00691571.
PIR	S36842.
RefSeq	NP_001076900.1.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000015388; ENSBTAP00000015388; ENSBTAG00000011583; Bos taurus. [Genome view]
GeneID	514666.
KEGG	bta:514666.
Organism-specific databases
CTD	514666.
Phylogenomic databases
HOVERGEN	P80227.
Enzyme and pathway databases
BRENDA	3.4.19.1. 251.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACPH_BOVIN

Accession

Primary (citable) accession number: P80227
Secondary accession number(s): A4FUX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 10, 2009
Last modified:	September 1, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)