P80225 (B4GT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4-galactosyltransferase 1
Short name=Beta-1,4-GalTase 1
Short name=Beta4Gal-T1
Short name=b4Gal-T1
EC=2.4.1.-

Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Including the following 4 domains:

1. Lactose synthase A protein
   EC=2.4.1.22
2. N-acetyllactosamine synthase
   EC=2.4.1.90
   Alternative name(s):
   Nal synthase
3. Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
   EC=2.4.1.38
4. Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
   EC=2.4.1.-

Gene names

Name:	B4galt1
Synonyms:	Ggtb2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	32 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Catalytic activity	UDP-alpha-D-galactose + D-glucose = UDP + lactose. UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide. UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	oligosaccharide biosynthetic process Traceable author statement PubMed 12098497. Source: RGD protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway response to wounding Inferred from expression pattern PubMed 12098497. Source: RGD
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Inferred from electronic annotation. Source: EC lactose synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›32	›32	Beta-1,4-galactosyltransferase 1		PRO_0000080531
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-terminal residue	32	1

Sequences

	Sequence		Length	Mass (Da)
	P80225 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: F58B846F5DCB297F	FASTA	32	3,137
	10 20 30 PGATLQXAXX LLVAVXAXPP PPLGVXPKPX PG

References

[1]	"Proteins of the Golgi apparatus. Purification to homogeneity, N-terminal sequence, and unusually large Stokes radius of the membrane-bound form of UDP-galactose:N-acetylglucosamine beta 1-4galactosyltransferase from rat liver." Bendiak B., Ward L.D., Simpson R.J. Eur. J. Biochem. 216:405-417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
IPI	IPI00195511.
PIR	S36825.
UniGene	Rn.17157.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:620900. rat.
Organism-specific databases
RGD	620900. B4galt1.
Enzyme and pathway databases
SABIO-RK	P80225.
UniPathway	UPA00378.
Gene expression databases
Genevestigator	P80225.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

B4GT1_RAT

Accession

Primary (citable) accession number: P80225

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program