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P80225

- B4GT1_RAT

UniProt

P80225 - B4GT1_RAT

Protein

Beta-1,4-galactosyltransferase 1

Gene

B4galt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This protein is responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

    Catalytic activityi

    UDP-alpha-D-galactose + D-glucose = UDP + lactose.
    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

    Cofactori

    Manganese.By similarity

    Pathwayi

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
    2. lactose synthase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. oligosaccharide biosynthetic process Source: RGD
    2. protein glycosylation Source: UniProtKB-UniPathway
    3. response to wounding Source: RGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP80225.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 1 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 1
    Short name:
    Beta4Gal-T1
    Short name:
    b4Gal-T1
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
    Including the following 4 domains:
    Lactose synthase A protein (EC:2.4.1.22)
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4galt1
    Synonyms:Ggtb2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620900. B4galt1.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
    Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. Golgi cisterna membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›32›32Beta-1,4-galactosyltransferase 1PRO_0000080531Add
    BLAST

    Post-translational modificationi

    The soluble form derives from the membrane form by proteolytic processing.

    Expressioni

    Gene expression databases

    GenevestigatoriP80225.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Sequencei

    Sequence statusi: Fragments.

    P80225-1 [UniParc]FASTAAdd to Basket

    « Hide

    PGATLQXAXX LLVAVXAXPP PPLGVXPKPX PG                      32
    Length:32
    Mass (Da):3,137
    Last modified:October 1, 1993 - v1
    Checksum:iF58B846F5DCB297F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi15 – 162Curated
    Non-terminal residuei32 – 321

    Sequence databases

    PIRiS36825.
    UniGeneiRn.17157.

    Genome annotation databases

    UCSCiRGD:620900. rat.

    Cross-referencesi

    Sequence databases

    PIRi S36825.
    UniGenei Rn.17157.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620900. rat.

    Organism-specific databases

    RGDi 620900. B4galt1.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    SABIO-RK P80225.

    Gene expression databases

    Genevestigatori P80225.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Proteins of the Golgi apparatus. Purification to homogeneity, N-terminal sequence, and unusually large Stokes radius of the membrane-bound form of UDP-galactose:N-acetylglucosamine beta 1-4galactosyltransferase from rat liver."
      Bendiak B., Ward L.D., Simpson R.J.
      Eur. J. Biochem. 216:405-417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Liver.

    Entry informationi

    Entry nameiB4GT1_RAT
    AccessioniPrimary (citable) accession number: P80225
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3