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P80225

- B4GT1_RAT

UniProt

P80225 - B4GT1_RAT

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Protein

Beta-1,4-galactosyltransferase 1

Gene

B4galt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This protein is responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Catalytic activityi

UDP-alpha-D-galactose + D-glucose = UDP + lactose.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  2. lactose synthase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. N-acetyllactosamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. oligosaccharide biosynthetic process Source: RGD
  2. protein glycosylation Source: UniProtKB-UniPathway
  3. response to wounding Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

SABIO-RKP80225.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 1 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 1
Short name:
Beta4Gal-T1
Short name:
b4Gal-T1
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
Including the following 4 domains:
Lactose synthase A protein (EC:2.4.1.22)
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt1
Synonyms:Ggtb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620900. B4galt1.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›32›32Beta-1,4-galactosyltransferase 1PRO_0000080531Add
BLAST

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Expressioni

Gene expression databases

GenevestigatoriP80225.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Sequencei

Sequence statusi: Fragments.

P80225-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30 
PGATLQXAXX LLVAVXAXPP PPLGVXPKPX PG
Length:32
Mass (Da):3,137
Last modified:October 1, 1993 - v1
Checksum:iF58B846F5DCB297F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi15 – 162Curated
Non-terminal residuei32 – 321

Sequence databases

PIRiS36825.
UniGeneiRn.17157.

Genome annotation databases

UCSCiRGD:620900. rat.

Cross-referencesi

Sequence databases

PIRi S36825.
UniGenei Rn.17157.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620900. rat.

Organism-specific databases

RGDi 620900. B4galt1.

Enzyme and pathway databases

UniPathwayi UPA00378 .
SABIO-RK P80225.

Gene expression databases

Genevestigatori P80225.

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Proteins of the Golgi apparatus. Purification to homogeneity, N-terminal sequence, and unusually large Stokes radius of the membrane-bound form of UDP-galactose:N-acetylglucosamine beta 1-4galactosyltransferase from rat liver."
    Bendiak B., Ward L.D., Simpson R.J.
    Eur. J. Biochem. 216:405-417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Liver.

Entry informationi

Entry nameiB4GT1_RAT
AccessioniPrimary (citable) accession number: P80225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3