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Protein

Duodenase-1

Gene

BDMD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease which has both trypsin-like and chymotrypsin-like activities.1 Publication

Catalytic activityi

Preferential cleavage: Lys-, Arg-, Tyr-, Phe-, Leu-.

pH dependencei

Optimum pH is 8.0.

Temperature dependencei

Optimum temperature is 50 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Charge relay system1
Active sitei107Charge relay system1
Active sitei201Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.B3. 908.
SABIO-RKP80219.

Protein family/group databases

MEROPSiS01.142.

Names & Taxonomyi

Protein namesi
Recommended name:
Duodenase-1 (EC:3.4.21.-)
Alternative name(s):
Duodenase I
Duodenum serine protease
Gene namesi
Name:BDMD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000024439618 – 193 Publications2
ChainiPRO_000008865920 – 251Duodenase-1Add BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi48 ↔ 64
Glycosylationi70N-linked (GlcNAc...)1
Disulfide bondi141 ↔ 207
Disulfide bondi172 ↔ 186

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP80219.
PeptideAtlasiP80219.
PRIDEiP80219.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000050126.

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 54Combined sources21
Beta strandi57 – 60Combined sources4
Helixi62 – 64Combined sources3
Beta strandi69 – 74Combined sources6
Beta strandi86 – 95Combined sources10
Turni101 – 103Combined sources3
Beta strandi109 – 115Combined sources7
Beta strandi140 – 146Combined sources7
Beta strandi148 – 150Combined sources3
Beta strandi160 – 167Combined sources8
Helixi170 – 173Combined sources4
Turni181 – 183Combined sources3
Beta strandi184 – 188Combined sources5
Beta strandi204 – 207Combined sources4
Beta strandi210 – 217Combined sources8
Beta strandi226 – 230Combined sources5
Helixi231 – 233Combined sources3
Helixi235 – 241Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EUFX-ray2.40A20-246[»]
ProteinModelPortaliP80219.
SMRiP80219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80219.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 242Peptidase S1PROSITE-ProRule annotationAdd BLAST223

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP80219.
KOiK01353.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80219-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLLLLVAL LSPTGEAGKI IGGHEAKPHS RPYMAFLLFK TSGKSHICGG
60 70 80 90 100
FLVREDFVLT AAHCLGSSIN VTLGAHNIME RERTQQVIPV RRPIPHPDYN
110 120 130 140 150
DETLANDIML LKLTRKADIT DKVSPINLPR SLAEVKPGMM CSVAGWGRLG
160 170 180 190 200
VNMPSTDNLQ EVDLEVQSEE KCIARFKNYI PFTQICAGDP SKRKNSFSGD
210 220 230 240 250
SGGPLVCNGV AQGIVSYGRN DGTTPDVYTR ISSFLSWIHS TMRRYKRQGS

V
Length:251
Mass (Da):27,556
Last modified:June 27, 2006 - v2
Checksum:i2392608CF8BF52B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68Missing AA sequence (PubMed:7867649).Curated1
Sequence conflicti158N → K AA sequence (PubMed:7867649).Curated1
Sequence conflicti194K → R AA sequence (PubMed:7867649).Curated1
Sequence conflicti194K → R AA sequence (PubMed:1425193).Curated1
Sequence conflicti219R → K AA sequence (PubMed:7867649).Curated1
Sequence conflicti236S → P AA sequence (PubMed:7867649).Curated1
Sequence conflicti239 – 245HSTMRRY → KRVMYLF AA sequence (PubMed:7867649).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198965 mRNA. Translation: AAG28537.1.
PIRiS69370.
S69371.
RefSeqiNP_776721.1. NM_174296.2.
UniGeneiBt.7145.

Genome annotation databases

GeneIDi281731.
KEGGibta:281731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198965 mRNA. Translation: AAG28537.1.
PIRiS69370.
S69371.
RefSeqiNP_776721.1. NM_174296.2.
UniGeneiBt.7145.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EUFX-ray2.40A20-246[»]
ProteinModelPortaliP80219.
SMRiP80219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000050126.

Protein family/group databases

MEROPSiS01.142.

Proteomic databases

PaxDbiP80219.
PeptideAtlasiP80219.
PRIDEiP80219.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281731.
KEGGibta:281731.

Organism-specific databases

CTDi3002.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP80219.
KOiK01353.

Enzyme and pathway databases

BRENDAi3.4.21.B3. 908.
SABIO-RKP80219.

Miscellaneous databases

EvolutionaryTraceiP80219.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDN1_BOVIN
AccessioniPrimary (citable) accession number: P80219
Secondary accession number(s): Q9GLN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 27, 2006
Last modified: November 30, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.