P80217 (IN35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon-induced 35 kDa protein
Short name=IFP 35
Short name=Ifi-35

Gene names

Name:	IFI35
Synonyms:	IFP35

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not yet known.
Subunit structure	Homodimer. Also interacts with B-ATF.
Subcellular location	Nucleus. Note: Nuclear following IFN treatment.
Tissue specificity	In a wide range of cell types, including fibroblasts, macrophages, and epithelial cells.
Induction	By interferon gamma.
Sequence similarities	Belongs to the NMI family.
Sequence caution	The sequence AAB61703.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	response to virus Non-traceable author statement. Source: UniProtKB type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CLEC4G	Q6UXB4	4	EBI-2115067,EBI-2114729

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P80217-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: P80217-2) The sequence of this isoform differs from the canonical sequence as follows:      126-126: M → VMM
Note: Due to a polymorphism at the 3'-splice acceptor site of intron 4.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Natural variant	128	1	M → V. Corresponds to variant rs588703 [ dbSNP | Ensembl ].

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 286	285	Interferon-induced 35 kDa protein		PRO_0000159704
Regions
	Domain	5 – 26	22	Leucine-zipper
Natural variations
	Alternative sequence	126	1	M → VMM in isoform 2.		VSP_003569
	Natural variant	126	1	M → V. Ref.5 Corresponds to variant rs588703 [ dbSNP | Ensembl ].		VAR_063758
Experimental info
	Sequence conflict	264 – 286	23	EVEAL…TSESG → GRGPDSRTPRTAGPSSLHL no nucleotide entry Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 2, 2010. Version 5. Checksum: 3FCAB26E97317508	FASTA	286	31,546
	10 20 30 40 50 60 MSAPLDAALH ALQEEQARLK MRLWDLQQLR KELGDSPKDK VPFSVPKIPL VFRGHTQQDP 70 80 90 100 110 120 EVPKSLVSNL RIHCPLLAGS ALITFDDPKV AEQVLQQKEH TINMEECRLR VQVQPLELPM 130 140 150 160 170 180 VTTIQMSSQL SGRRVLVTGF PASLRLSEEE LLDKLEIFFG KTRNGGGDVD VRELLPGSVM 190 200 210 220 230 240 LGFARDGVAQ RLCQIGQFTV PLGGQQVPLR VSPYVNGEIQ KAEIRSQPVP RSVLVLNIPD 250 260 270 280 ILDGPELHDV LEIHFQKPTR GGGEVEALTV VPQGQQGLAV FTSESG
	Isoform 2 [UniParc]. Checksum: F8C385872AF39D6D	FASTA	288	31,777
	10 20 30 40 50 60 MSAPLDAALH ALQEEQARLK MRLWDLQQLR KELGDSPKDK VPFSVPKIPL VFRGHTQQDP 70 80 90 100 110 120 EVPKSLVSNL RIHCPLLAGS ALITFDDPKV AEQVLQQKEH TINMEECRLR VQVQPLELPM 130 140 150 160 170 180 VTTIQVMMSS QLSGRRVLVT GFPASLRLSE EELLDKLEIF FGKTRNGGGD VDVRELLPGS 190 200 210 220 230 240 VMLGFARDGV AQRLCQIGQF TVPLGGQQVP LRVSPYVNGE IQKAEIRSQP VPRSVLVLNI 250 260 270 280 PDILDGPELH DVLEIHFQKP TRGGGEVEAL TVVPQGQQGL AVFTSESG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IFP 35 is an interferon-induced leucine zipper protein that undergoes interferon-regulated cellular redistribution." Bange F.-C., Vogel U., Flohr T., Kiekenbeck M., Denecke B., Boettger E.C. J. Biol. Chem. 269:1091-1098(1994) [PubMed: 8288566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[2]	"IFP 35 forms complexes with B-ATF, a member of the AP1 family of transcription factors." Wang X., Johansen L.M., Tae H.-J., Taparowsky E.J. Biochem. Biophys. Res. Commun. 229:316-322(1996) [PubMed: 8954125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT VAL-128 (ISOFORM 2).
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-128 (ISOFORM 2). Tissue: Skin.
[5]	"Complete genomic sequence and analysis of 117 kb of human DNA containing the gene BRCA1." Smith T.M., Lee M.K., Szabo C.I., Jerome N., McEuen M., Taylor M., Hood L., King M.-C. Genome Res. 6:1029-1049(1996) [PubMed: 8938427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-283 (ISOFORM 1), VARIANT VAL-126.
[6]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-18. Tissue: Platelet.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72882 mRNA. Translation: AAB61703.1. Sequence problems. AC055866 Genomic DNA. No translation available. BC001356 mRNA. Translation: AAH01356.1. L78833 Genomic DNA. Translation: AAC37597.1.
IPI	IPI00008613. IPI00218475.
PIR	JC5262.
RefSeq	NP_005524.2. NM_005533.4.
UniGene	Hs.632258.
3D structure databases
ProteinModelPortal	P80217.
SMR	P80217. Positions 76-284.
ModBase	Search...
Protein-protein interaction databases
IntAct	P80217. 3 interactions.
STRING	P80217.
PTM databases
PhosphoSite	P80217.
Polymorphism databases
DMDM	311033494.
Proteomic databases
PRIDE	P80217.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000415816; ENSP00000394579; ENSG00000068079.
GeneID	3430.
KEGG	hsa:3430.
UCSC	uc010cyv.1. human.
Organism-specific databases
CTD	3430.
GeneCards	GC17P041158.
HGNC	HGNC:5399. IFI35.
MIM	600735. gene.
neXtProt	NX_P80217.
PharmGKB	PA29645.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09438.
GeneTree	ENSGT00530000063686.
HOVERGEN	HBG052125.
InParanoid	P80217.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P80217.
Bgee	P80217.
CleanEx	HS_IFI35.
Genevestigator	P80217.
GermOnline	ENSG00000068079. Homo sapiens.
Family and domain databases
InterPro	IPR009938. Interferon_induced_35kDa_N. IPR009909. Nmi/IFP35. [Graphical view]
Pfam	PF07334. IFP_35_N. 1 hit. PF07292. NID. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	13524.
SOURCE	Search...

Entry information

Entry name

IN35_HUMAN

Accession

Primary (citable) accession number: P80217
Secondary accession number(s): C9JGX1 , Q92984, Q99537, Q9BV98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 2, 2010
Last modified:	January 25, 2012
This is version 93 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.