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Protein

Adenylosuccinate synthetase

Gene

ADE12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.2 Publications

Miscellaneous

Present with 56800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Enzyme regulationi

Inhibited by GMP. Inhibited by chloride. Inhibited in a highly specific manner by the binding of a 44-base DNA oligonucleotide carrying the ARS core consensus sequence.1 Publication

Kineticsi

  1. KM=1650 µM for L-aspartate2 Publications
  2. KM=200 µM for IMP2 Publications
  3. KM=1650 µM for GTP2 Publications

    pH dependencei

    Optimum pH is 8.0.2 Publications

    Temperature dependencei

    Optimum temperature is 35 degrees Celsius.2 Publications

    Pathwayi: AMP biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Adenylosuccinate synthetase (ADE12)
    2. Adenylosuccinate lyase (ADE13)
    This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei12Proton acceptorUniRule annotation1
    Metal bindingi12MagnesiumUniRule annotation1
    Metal bindingi39Magnesium; via carbonyl oxygenUniRule annotation1
    Active sitei40Proton donorUniRule annotation1
    Binding sitei134IMPUniRule annotation1
    Binding sitei148IMP; shared with dimeric partnerUniRule annotation1
    Binding sitei230IMPUniRule annotation1
    Binding sitei245IMPUniRule annotation1
    Binding sitei309IMPUniRule annotation1
    Binding sitei311GTPUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 17GTPUniRule annotation7
    Nucleotide bindingi39 – 41GTPUniRule annotation3
    Nucleotide bindingi337 – 339GTPUniRule annotation3
    Nucleotide bindingi419 – 421GTPUniRule annotation3

    GO - Molecular functioni

    • adenylosuccinate synthase activity Source: SGD
    • DNA replication origin binding Source: SGD
    • GTP binding Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • 'de novo' AMP biosynthetic process Source: GO_Central
    • IMP metabolic process Source: GO_Central
    • purine nucleotide biosynthetic process Source: SGD

    Keywordsi

    Molecular functionLigase
    Biological processPurine biosynthesis
    LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YNL220W-MONOMER
    YEAST:YNL220W-MONOMER
    ReactomeiR-SCE-73817 Purine ribonucleoside monophosphate biosynthesis
    UniPathwayiUPA00075; UER00335

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
    Short name:
    AMPSaseUniRule annotation
    Short name:
    AS-synthetase
    Short name:
    AdSSUniRule annotation
    Alternative name(s):
    IMP--aspartate ligaseUniRule annotation
    Gene namesi
    Name:ADE12UniRule annotation
    Ordered Locus Names:YNL220W
    ORF Names:N1290
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIV

    Organism-specific databases

    EuPathDBiFungiDB:YNL220W
    SGDiS000005164 ADE12

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00000951382 – 433Adenylosuccinate synthetaseAdd BLAST432

    Proteomic databases

    MaxQBiP80210
    PaxDbiP80210
    PRIDEiP80210

    PTM databases

    iPTMnetiP80210

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation3 Publications

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi35616, 146 interactors
    DIPiDIP-4286N
    IntActiP80210, 8 interactors
    MINTiP80210
    STRINGi4932.YNL220W

    Structurei

    3D structure databases

    ProteinModelPortaliP80210
    SMRiP80210
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni12 – 15IMP bindingUniRule annotation4
    Regioni37 – 40IMP bindingUniRule annotation4
    Regioni305 – 311Substrate bindingUniRule annotation7

    Sequence similaritiesi

    Belongs to the adenylosuccinate synthetase family.UniRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00390000015553
    HOGENOMiHOG000260959
    InParanoidiP80210
    KOiK01939
    OMAiSNAGHTV
    OrthoDBiEOG092C2D4T

    Family and domain databases

    CDDicd03108 AdSS, 1 hit
    HAMAPiMF_00011 Adenylosucc_synth, 1 hit
    InterProiView protein in InterPro
    IPR018220 Adenylosuccin_syn_GTP-bd
    IPR033128 Adenylosuccin_syn_Lys_AS
    IPR001114 Adenylosuccinate_synthetase
    IPR027417 P-loop_NTPase
    PANTHERiPTHR11846 PTHR11846, 1 hit
    PfamiView protein in Pfam
    PF00709 Adenylsucc_synt, 1 hit
    SMARTiView protein in SMART
    SM00788 Adenylsucc_synt, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    TIGRFAMsiTIGR00184 purA, 1 hit
    PROSITEiView protein in PROSITE
    PS01266 ADENYLOSUCCIN_SYN_1, 1 hit
    PS00513 ADENYLOSUCCIN_SYN_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80210-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVNVVLGSQW GDEGKGKLVD LLVGKYDIVA RCAGGNNAGH TIVVDGVKYD
    60 70 80 90 100
    FHMLPSGLVN PNCQNLLGNG VVIHVPSFFK ELETLEAKGL KNARSRLFVS
    110 120 130 140 150
    SRAHLVFDFH QVTDKLRELE LSGRSKDGKN IGTTGKGIGP TYSTKASRSG
    160 170 180 190 200
    LRVHHLVNDQ PGAWEEFVAR YKRLLETRRQ RYGDFEYDFE AKLAEYKKLR
    210 220 230 240 250
    EQLKPFVVDS VVFMHNAIEA KKKILVEGAN ALMLDIDFGT YPYVTSSNTG
    260 270 280 290 300
    IGGVLTGLGI PPRTIDEIYG VVKAYTTRVG EGPFPTEQLN ENGEKLQTIG
    310 320 330 340 350
    AEFGVTTGRK RRCGWLDLVV LKYSTLINGY TSLNITKLDV LDTFKEIPVG
    360 370 380 390 400
    ISYSIQGKKL DLFPEDLNIL GKVEVEYKVL PGWDQDITKI TKYEDLPENA
    410 420 430
    KKYLKYIEDF VGVPVEWVGT GPARESMLHK EIK
    Length:433
    Mass (Da):48,279
    Last modified:January 23, 2007 - v3
    Checksum:i800E0F4062D9856F
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti237D → G AA sequence (PubMed:8376380).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L22185 Genomic DNA Translation: AAA91338.1
    Z48671 Genomic DNA Translation: CAA88590.1
    Z71496 Genomic DNA Translation: CAA96123.1
    BK006947 Genomic DNA Translation: DAA10336.1
    PIRiS48515
    RefSeqiNP_014179.1, NM_001183058.1

    Genome annotation databases

    EnsemblFungiiYNL220W; YNL220W; YNL220W
    GeneIDi855501
    KEGGisce:YNL220W

    Similar proteinsi

    Entry informationi

    Entry nameiPURA_YEAST
    AccessioniPrimary (citable) accession number: P80210
    Secondary accession number(s): D6W0X0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: June 20, 2018
    This is version 166 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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