Reviewed,
UniProtKB/Swiss-Prot P80210 (PURA_YEAST)
Last modified
November 25, 2008.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Adenylosuccinate synthetase Short name=AdSS EC=6.3.4.4 Alternative name(s): IMP--aspartate ligase AMPSase | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 433 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays an important role in the de novo pathway of purine nucleotide biosynthesis. |
| Catalytic activity | GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. |
| Cofactor | Binds 1 magnesium ion per subunit By similarity. |
| Enzyme regulation | Inhibited in a highly specific manner by the binding of a 44-base DNA oligonucleotide carrying the ARS core consensus sequence. |
| Pathway | Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
| Subunit structure | Homodimer By similarity. |
| Miscellaneous | Present with 56800 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the adenylosuccinate synthetase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Ligand | DNA-binding GTP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | purine nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from direct assay. Source: SGD |
| Molecular function | DNA replication origin binding Ref.2 Inferred from direct assay. Source: SGD GTP bindingInferred from electronic annotation. Source: InterPro adenylosuccinate synthase activity Ref.2Inferred from electronic annotation. Source: InterPro identical protein bindingInferred from physical interaction. Source: IntAct magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-14267,EBI-14267 | ||
| P43582 | 1 | EBI-14267,EBI-22766 | ||
| AUS1 | Q08409 | 1 | EBI-14267,EBI-35723 | |
| ESS1 | P22696 | 1 | EBI-14267,EBI-6679 | |
| PRP40 | P33203 | 1 | EBI-14267,EBI-701 | |
| RSP5 | P39940 | 1 | EBI-14267,EBI-16219 | |
| SET2 | P46995 | 1 | EBI-14267,EBI-16985 | |
| SSM4 | P40318 | 1 | EBI-14267,EBI-18208 | |
| URN1 | Q06525 | 1 | EBI-14267,EBI-35138 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 433 | 432 | Adenylosuccinate synthetase | PRO_0000095138 | |||||
Regions | |||||||||
| Nucleotide binding | 11 – 17 | 7 | GTP By similarity | ||||||
Sites | |||||||||
| Active site | 145 | 1 | By similarity | ||||||
| Active site | 152 | 1 | By similarity | ||||||
| Metal binding | 12 | 1 | Magnesium By similarity | ||||||
| Metal binding | 39 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 122 | 1 | Phosphoserine | ||||||
| Modified residue | 143 | 1 | Phosphoserine | ||||||
Experimental info | |||||||||
| Sequence conflict | 237 | 1 | D → G AA sequence Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Saccharomyces cerevisiae ADE12 gene, coding for adenylosuccinate synthetase (EC 6.3.4.4). Cloning, sequencing, expression, and superproduction." Andreichuk I.V., Shabes A.V., Ryzhova T.A., Kotova I.A., Domkin V.D. Mol. Genet. Mikrobiol. Virusol. 1:21-28(1995) [PubMed: 7603488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Enzymatic properties and inhibition by single-stranded autonomously replicating sequences of adenylosuccinate synthase from Saccharomyces cerevisiae." Gallert K.C., Ohanjan T., Daignan-Fornier B., Lottspeich F., Krauss G. Eur. J. Biochem. 239:487-493(1996) [PubMed: 8706758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INHIBITION BY SS-DNA. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Characterization of two novel single-stranded DNA-specific autonomously replicating sequence-binding proteins from Saccharomyces cerevisiae, one of which is adenylosuccinate synthetase." Zeidler R., Hobert O., Johannes L., Faulhammer H., Krauss G. J. Biol. Chem. 268:20191-20197(1993) [PubMed: 8376380] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-23 AND 234-245. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122 AND SER-143, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L22185 Genomic DNA. Translation: AAA91338.1. Z48671 Genomic DNA. Translation: CAA88590.1. Z71496 Genomic DNA. Translation: CAA96123.1. | |
| PIR | S48515. |
| RefSeq | NP_014179.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IWE based on UniProtKB P28650. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:4286N. |
| IntAct | P80210. |
Proteomic databases | |
| PeptideAtlas | P80210. |
Genome annotation databases | |
| Ensembl | YNL220W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 855501. |
| GenomeReviews | Gene locus YNL220W in contig Y13139_GR. |
| KEGG | sce:YNL220W. |
| NMPDR | fig|4932.3.peg.5244. |
Organism-specific databases | |
| CYGD | YNL220w. |
| SGD | S000005164. ADE12. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P80210. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MON-509. |
Gene expression databases | |
| ArrayExpress | P80210. |
| GermOnline | YNL220W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001114. AdlSucc_Synth. [Graphical view] |
| PANTHER | PTHR11846. Asucc_synthtase. 1 hit. |
| Pfam | PF00709. Adenylsucc_synt. 1 hit. [Graphical view] |
| ProDom | PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00788. Adenylsucc_synt. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00184. purA. 1 hit. |
| PROSITE | PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P80210. |
| NextBio | 979499. |
Entry information
| Entry name | PURA_YEAST | ||||||||
| Accession | Primary (citable) accession number: P80210 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

Clusters with


