ID   OTX2_MOUSE              Reviewed;         289 AA.
AC   P80206;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   11-NOV-2015, entry version 124.
DE   RecName: Full=Homeobox protein OTX2;
DE   AltName: Full=Orthodenticle homolog 2;
GN   Name=Otx2; Synonyms=Otx-2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=8101484;
RA   Simeone A., Acampora D., Mallamaci A., Stornaiuolo A., D'Apice M.R.,
RA   Nigro V., Boncinelli E.;
RT   "A vertebrate gene related to orthodenticle contains a homeodomain of
RT   the bicoid class and demarcates anterior neuroectoderm in the
RT   gastrulating mouse embryo.";
RL   EMBO J. 12:2735-2747(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 31-98.
RC   TISSUE=Embryo;
RX   PubMed=1353865; DOI=10.1038/358687a0;
RA   Simeone A., Acampora D., Gulisano M., Stornaiuolo A., Boncinelli E.;
RT   "Nested expression domains of four homeobox genes in developing
RT   rostral brain.";
RL   Nature 358:687-690(1992).
RN   [3]
RP   MUTAGENESIS OF ASN-225.
RX   PubMed=18728160; DOI=10.1210/jc.2008-1189;
RA   Diaczok D., Romero C., Zunich J., Marshall I., Radovick S.;
RT   "A novel dominant negative mutation of OTX2 associated with combined
RT   pituitary hormone deficiency.";
RL   J. Clin. Endocrinol. Metab. 93:4351-4359(2008).
RN   [4]
RP   STRUCTURE BY NMR OF 39-105.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the homeobox domain of homeobox protein OTX2.";
RL   Submitted (OCT-2006) to the PDB data bank.
CC   -!- FUNCTION: Probably plays a role in the development of the brain
CC       and the sense organs. Can bind to the BCD target sequence (BTS):
CC       5'-TCTAATCCC-3'.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Brain: restricted regions of the developing
CC       rostral brain including the presumptive cerebral cortex and
CC       olfactory bulbs; expressed in the developing olfactory, auricolar
CC       and ocular systems, including the covering of the optic nerve.
CC   -!- DEVELOPMENTAL STAGE: Embryo.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid
CC       subfamily. {ECO:0000305}.
CC   -!- SIMILARITY: Contains 1 homeobox DNA-binding domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00108}.
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DR   EMBL; X68884; CAA48755.1; -; mRNA.
DR   CCDS; CCDS49473.1; -.
DR   PIR; S35346; S35346.
DR   UniGene; Mm.134516; -.
DR   UniGene; Mm.487292; -.
DR   PDB; 2DMS; NMR; -; A=39-105.
DR   PDBsum; 2DMS; -.
DR   ProteinModelPortal; P80206; -.
DR   SMR; P80206; 39-106.
DR   MINT; MINT-1347226; -.
DR   STRING; 10090.ENSMUSP00000113690; -.
DR   PhosphoSite; P80206; -.
DR   PaxDb; P80206; -.
DR   PRIDE; P80206; -.
DR   MGI; MGI:97451; Otx2.
DR   eggNOG; KOG2251; Eukaryota.
DR   eggNOG; ENOG4111XIJ; LUCA.
DR   HOGENOM; HOG000082677; -.
DR   HOVERGEN; HBG004028; -.
DR   InParanoid; P80206; -.
DR   PhylomeDB; P80206; -.
DR   ChiTaRS; Otx2; mouse.
DR   EvolutionaryTrace; P80206; -.
DR   PRO; PR:P80206; -.
DR   Proteomes; UP000000589; Unplaced.
DR   CleanEx; MM_OTX2; -.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0030426; C:growth cone; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0043234; C:protein complex; ISO:MGI.
DR   GO; GO:0046982; F:protein heterodimerization activity; IPI:MGI.
DR   GO; GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; ISO:MGI.
DR   GO; GO:0001077; F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding; ISO:MGI.
DR   GO; GO:0048856; P:anatomical structure development; IGI:MGI.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR   GO; GO:0007411; P:axon guidance; ISO:MGI.
DR   GO; GO:0030154; P:cell differentiation; IMP:MGI.
DR   GO; GO:0045165; P:cell fate commitment; IMP:MGI.
DR   GO; GO:0001708; P:cell fate specification; IMP:MGI.
DR   GO; GO:0007417; P:central nervous system development; IMP:MGI.
DR   GO; GO:0048852; P:diencephalon morphogenesis; IGI:MGI.
DR   GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR   GO; GO:0007492; P:endoderm development; IGI:MGI.
DR   GO; GO:0042706; P:eye photoreceptor cell fate commitment; IMP:MGI.
DR   GO; GO:0030900; P:forebrain development; IMP:MGI.
DR   GO; GO:0042472; P:inner ear morphogenesis; IGI:MGI.
DR   GO; GO:0022037; P:metencephalon development; IGI:MGI.
DR   GO; GO:0030901; P:midbrain development; IMP:MGI.
DR   GO; GO:0030182; P:neuron differentiation; IMP:MGI.
DR   GO; GO:0048663; P:neuron fate commitment; IMP:MGI.
DR   GO; GO:0048664; P:neuron fate determination; IMP:MGI.
DR   GO; GO:0009887; P:organ morphogenesis; IMP:MGI.
DR   GO; GO:0040019; P:positive regulation of embryonic development; IMP:UniProtKB.
DR   GO; GO:2000543; P:positive regulation of gastrulation; IMP:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0090009; P:primitive streak formation; IMP:UniProtKB.
DR   GO; GO:0006461; P:protein complex assembly; ISO:MGI.
DR   GO; GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.
DR   GO; GO:0032525; P:somite rostral/caudal axis specification; IMP:MGI.
DR   GO; GO:0006366; P:transcription from RNA polymerase II promoter; ISO:MGI.
DR   Gene3D; 1.10.10.60; -; 1.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR009057; Homeodomain-like.
DR   InterPro; IPR003022; Otx2_TF.
DR   InterPro; IPR003025; Otx_TF.
DR   InterPro; IPR013851; Otx_TF_C.
DR   Pfam; PF00046; Homeobox; 1.
DR   Pfam; PF03529; TF_Otx; 1.
DR   PRINTS; PR01257; OTX2HOMEOBOX.
DR   PRINTS; PR01255; OTXHOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Developmental protein; DNA-binding;
KW   Homeobox; Nucleus; Reference proteome.
FT   CHAIN         1    289       Homeobox protein OTX2.
FT                                /FTId=PRO_0000049211.
FT   DNA_BIND     38     97       Homeobox. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00108}.
FT   MUTAGEN     225    225       N->S: Has a dominant negative effect on
FT                                the proximal promoter region of HESX1
FT                                gene; represses reporter expression.
FT                                {ECO:0000269|PubMed:18728160}.
FT   HELIX        47     59
FT   HELIX        65     74
FT   HELIX        79     90
FT   HELIX        92     95
SQ   SEQUENCE   289 AA;  31622 MW;  B4EA3A5BF83E2D99 CRC64;
     MMSYLKQPPY AVNGLSLTTS GMDLLHPSVG YPATPRKQRR ERTTFTRAQL DVLEALFAKT
     RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQQQ QNGGQNKVRP AKKKSSPARE
     VSSESGTSGQ FTPPSSTSVP TIASSSAPVS IWSPASISPL SDPLSTSSSC MQRSYPMTYT
     QASGYSQGYA GSTSYFGGMD CGSYLTPMHH QLPGPGATLS PMGTNAVTSH LNQSPASLST
     QGYGASSLGF NSTTDCLDYK DQTASWKLNF NADCLDYKDQ TSSWKFQVL
//