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P80206 (OTX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein OTX2
Alternative name(s):
Orthodenticle homolog 2
Gene names
Name:Otx2
Synonyms:Otx-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Subcellular location

Nucleus Probable.

Tissue specificity

Brain: restricted regions of the developing rostral brain including the presumptive cerebral cortex and olfactory bulbs; expressed in the developing olfactory, auricolar and ocular systems, including the covering of the optic nerve.

Developmental stage

Embryo.

Sequence similarities

Belongs to the paired homeobox family. Bicoid subfamily.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanatomical structure development

Inferred from genetic interaction PubMed 11412024. Source: MGI

anterior/posterior pattern specification

Inferred from mutant phenotype PubMed 12652306PubMed 9501024. Source: MGI

cell differentiation

Inferred from mutant phenotype PubMed 14625556. Source: MGI

cell fate commitment

Inferred from mutant phenotype PubMed 15105370. Source: MGI

cell fate specification

Inferred from mutant phenotype PubMed 15888661. Source: MGI

central nervous system development

Inferred from mutant phenotype PubMed 11731459PubMed 12652306. Source: MGI

diencephalon morphogenesis

Inferred from genetic interaction PubMed 20816794. Source: MGI

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 12652306. Source: MGI

endoderm development

Inferred from genetic interaction PubMed 11412024. Source: MGI

eye photoreceptor cell fate commitment

Inferred from mutant phenotype PubMed 14625556. Source: MGI

forebrain development

Inferred from mutant phenotype PubMed 14625556PubMed 15201223. Source: MGI

inner ear morphogenesis

Inferred from genetic interaction PubMed 10225993. Source: MGI

metencephalon development

Inferred from genetic interaction PubMed 20816794. Source: MGI

midbrain development

Inferred from mutant phenotype PubMed 15201223PubMed 15888661. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 15888661. Source: MGI

neuron fate commitment

Inferred from mutant phenotype PubMed 16339193. Source: MGI

organ morphogenesis

Inferred from mutant phenotype PubMed 14625556. Source: MGI

positive regulation of embryonic development

Inferred from mutant phenotype PubMed 11291865. Source: UniProtKB

positive regulation of gastrulation

Inferred from mutant phenotype PubMed 11291865. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16539743. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 11291865. Source: UniProtKB

primitive streak formation

Inferred from mutant phenotype PubMed 11291865. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15105370. Source: MGI

somite rostral/caudal axis specification

Inferred from mutant phenotype PubMed 11291865. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 10891582. Source: MGI

nucleus

Inferred from direct assay PubMed 10891582PubMed 19796622PubMed 22992956. Source: MGI

   Molecular_functionprotein heterodimerization activity

Inferred from physical interaction PubMed 15890343. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289Homeobox protein OTX2
PRO_0000049211

Regions

DNA binding38 – 9760Homeobox

Experimental info

Mutagenesis2251N → S: Has a dominant negative effect on the proximal promoter region of HESX1 gene; represses reporter expression. Ref.3

Secondary structure

......... 289
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P80206 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: B4EA3A5BF83E2D99

FASTA28931,622
        10         20         30         40         50         60 
MMSYLKQPPY AVNGLSLTTS GMDLLHPSVG YPATPRKQRR ERTTFTRAQL DVLEALFAKT 

        70         80         90        100        110        120 
RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQQQ QNGGQNKVRP AKKKSSPARE 

       130        140        150        160        170        180 
VSSESGTSGQ FTPPSSTSVP TIASSSAPVS IWSPASISPL SDPLSTSSSC MQRSYPMTYT 

       190        200        210        220        230        240 
QASGYSQGYA GSTSYFGGMD CGSYLTPMHH QLPGPGATLS PMGTNAVTSH LNQSPASLST 

       250        260        270        280 
QGYGASSLGF NSTTDCLDYK DQTASWKLNF NADCLDYKDQ TSSWKFQVL 

« Hide

References

[1]"A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo."
Simeone A., Acampora D., Mallamaci A., Stornaiuolo A., D'Apice M.R., Nigro V., Boncinelli E.
EMBO J. 12:2735-2747(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"Nested expression domains of four homeobox genes in developing rostral brain."
Simeone A., Acampora D., Gulisano M., Stornaiuolo A., Boncinelli E.
Nature 358:687-690(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-98.
Tissue: Embryo.
[3]"A novel dominant negative mutation of OTX2 associated with combined pituitary hormone deficiency."
Diaczok D., Romero C., Zunich J., Marshall I., Radovick S.
J. Clin. Endocrinol. Metab. 93:4351-4359(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASN-225.
[4]"Solution structure of the homeobox domain of homeobox protein OTX2."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 39-105.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68884 mRNA. Translation: CAA48755.1.
PIRS35346.
UniGeneMm.134516.
Mm.487292.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DMSNMR-A39-105[»]
ProteinModelPortalP80206.
SMRP80206. Positions 12-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1347226.

PTM databases

PhosphoSiteP80206.

Proteomic databases

PRIDEP80206.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:97451. Otx2.

Phylogenomic databases

eggNOGNOG245767.
HOGENOMHOG000082677.
HOVERGENHBG004028.
PhylomeDBP80206.

Gene expression databases

CleanExMM_OTX2.
GenevestigatorP80206.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003022. Otx2_TF.
IPR003025. Otx_TF.
IPR013851. Otx_TF_C.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF03529. TF_Otx. 1 hit.
[Graphical view]
PRINTSPR01257. OTX2HOMEOBOX.
PR01255. OTXHOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSOTX2. mouse.
EvolutionaryTraceP80206.
PROP80206.
SOURCESearch...

Entry information

Entry nameOTX2_MOUSE
AccessionPrimary (citable) accession number: P80206
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot