Reviewed, UniProtKB/Swiss-Prot P80204 (TGFR1_RAT)

Names and origin

Protein names

Recommended name:
    TGF-beta receptor type-1
    EC=2.7.11.30
Alternative name(s):
    Transforming growth factor-beta receptor type I
      Short name=TGF-beta receptor type I
    TGF-beta type I receptor
    TbetaR-I
    TGFR-1
    Serine/threonine-protein kinase receptor R4
      Short name=SKR4

Gene names

Name:

Tgfbr1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
Catalytic activity	ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Interacts with CD109. The unphosphorylated protein interacts with FKBP1A and is stabilized the inactive conformation. Phosphorylation of the GS region abrogates FKBP1A binding. Interacts with SMAD2 when phosphorylated on several residues in the GS region By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Urogenital ridge, testis, ovary, brain and lungs.
Post-translational modification	Phosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 GS domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	aging Inferred from expression pattern. Source: RGD embryo implantation Inferred from expression pattern. Source: RGD gut development Inferred from expression pattern. Source: RGD lung development Inferred from expression pattern. Source: RGD negative regulation of endothelial cell differentiation Inferred from mutant phenotype. Source: RGD organ regeneration Inferred from expression pattern. Source: RGD protein amino acid autophosphorylation Inferred from mutant phenotype. Source: RGD response to electrical stimulus Inferred from expression pattern. Source: RGD response to estrogen stimulus Inferred from direct assay. Source: RGD response to hypoxia Inferred from expression pattern. Source: RGD response to organic cyclic substance Inferred from expression pattern. Source: RGD response to prostaglandin E stimulus Inferred from expression pattern. Source: RGD response to toxin Inferred from expression pattern. Source: RGD transforming growth factor beta receptor signaling pathway Inferred from mutant phenotype. Source: RGD
Cellular component	apical plasma membrane Inferred from direct assay. Source: RGD basolateral plasma membrane Inferred from direct assay. Source: RGD transforming growth factor beta receptor complex Inferred by curator. Source: RGD
Molecular function	ATP binding Inferred from mutant phenotype. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein complex binding Inferred from physical interaction. Source: RGD protein heterodimerization activity Inferred from mutant phenotype. Source: RGD transforming growth factor beta binding Inferred from direct assay. Source: RGD transforming growth factor beta receptor activity, type I Inferred from mutant phenotype. Source: RGD ubiquitin protein ligase binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 501	479	TGF-beta receptor type-1		PRO_0000024425
Regions
	Topological domain	23 – 123	101	Extracellular Potential
	Transmembrane	124 – 146	23	Potential
	Topological domain	147 – 501	355	Cytoplasmic Potential
	Domain	173 – 202	30	GS
	Domain	203 – 493	291	Protein kinase
	Nucleotide binding	209 – 217	9	ATP By similarity
Sites
	Active site	331	1	Proton acceptor By similarity
	Binding site	230	1	ATP By similarity
Amino acid modifications
	Modified residue	183	1	Phosphothreonine By similarity
	Modified residue	184	1	Phosphothreonine By similarity
	Modified residue	185	1	Phosphoserine By similarity
	Modified residue	187	1	Phosphoserine By similarity
	Modified residue	189	1	Phosphoserine By similarity
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 50		By similarity
	Disulfide bond	34 ↔ 37		By similarity
	Disulfide bond	44 ↔ 67		By similarity
	Disulfide bond	82 ↔ 94		By similarity
	Disulfide bond	95 ↔ 100		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80204-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: EAD7F8D37E456102	FASTA	501	56,000
	10 20 30 40 50 60 MEAASAALRR CLLLIVLVAA ATLLPGAKAL QCFCHLCTKD NFTCETDGLC FVSVTETTDK 70 80 90 100 110 120 VIHNSMCIAE IDLIPRDRPF VCAPSSKTGA VTYCCNQDHC NKIELPTTGP FSEKQSAGLG 130 140 150 160 170 180 PVELAAVIAG PVCFVCIALM LMVYICHNRT VIHHRVPNEE DPSLDRPFIS EGTTLKDLIY 190 200 210 220 230 240 DMTTSGSGSG LPLLVQRTIA RTIVLQESIG KGRFGEVWRG KWRGEEVAVK IFSSREERSW 250 260 270 280 290 300 FREAEIYQTV MLRHENILGF IAADNKDNGT WTQLWLVSDY HEHGSLFDYL NRYTVTVEGM 310 320 330 340 350 360 IKLALSTASG LAHLHMEIVG TQGKPAIAHR DLKSKNILVK KNGTCCIADL GLAVRHDSAT 370 380 390 400 410 420 DTIDIAPNHR VGTKRYMAPE VLDDSINMKH FESFKRADIY AMGLVFWEIA RRCSIGGIHE 430 440 450 460 470 480 DYQLPYYDLV PSDPSVEEMR KVVCEQKLRP NIPNRWQSCE ALRVMAKIMR ECWYANGAAR 490 500 LTALRIKKTL SQLSQQEGIK M

References

[1]	"Developmental expression of four novel serine/threonine kinase receptors homologous to the activin/transforming growth factor-beta type II receptor family." He W.-W., Gustafson M.L., Hirobe S., Donahoe P.K. Dev. Dyn. 196:133-142(1993) [PubMed: 8395914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Urogenital ridge.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26110 mRNA. Translation: AAA83216.1.
IPI	IPI00195461.
UniGene	Rn.44402
3D structure databases
HSSP	HSSP built from PDB template 1IAS based on UniProtKB P36897.
SMR	P80204. Positions 199-501.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000007036. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	3852. Tgfbr1.
Phylogenomic databases
HOVERGEN	P80204.
Enzyme and pathway databases
BRENDA	2.7.10.2. 248. 2.7.11.30. 248.
Gene expression databases
ArrayExpress	P80204.
GermOnline	ENSRNOG00000007036. Rattus norvegicus.
Family and domain databases
InterPro	IPR000472. Activin_rcpt. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR003605. TGF_beta_rcpt_GS. [Graphical view]
Pfam	PF01064. Activin_recp. 1 hit. PF00069. Pkinase. 1 hit. PF08515. TGF_beta_GS. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00467. GS. 1 hit. [Graphical view]
PROSITE	PS51256. GS. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGFR1_RAT

Accession

Primary (citable) accession number: P80204

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)