P80195 (GLCM1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosylation-dependent cell adhesion molecule 1
Short name=GlyCAM-1

Alternative name(s):
28 kDa milk glycoprotein PP3
Lactophorin
Proteose-peptone component 3
Short name=PP3

Gene names

Name:

GLYCAM1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Peripheral membrane protein.
Tissue specificity	Highly and specifically expressed in the lactating mammary gland.
Sequence similarities	Belongs to the PP3/GlyCAM-1 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: Compara membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sulfate binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.5 Ref.6
	Chain	19 – 153	135	Glycosylation-dependent cell adhesion molecule 1		PRO_0000025408
Amino acid modifications
	Modified residue	47	1	Phosphoserine Ref.2 Ref.5 Ref.7
	Modified residue	52	1	Phosphoserine Ref.2 Ref.5 Ref.7
	Modified residue	56	1	Phosphoserine Ref.2 Ref.5 Ref.7
	Modified residue	58	1	Phosphoserine Ref.2 Ref.5 Ref.7
	Modified residue	64	1	Phosphoserine Ref.2 Ref.5 Ref.7
	Glycosylation	34	1	O-linked (GalNAc...); partial Ref.2 Ref.5 Ref.7
	Glycosylation	78	1	O-linked (HexNAc...) Ref.7
	Glycosylation	95	1	N-linked (GlcNAc...) Ref.2 Ref.5 Ref.7
	Glycosylation	104	1	O-linked (GalNAc...) Ref.5 Ref.7		CAR_000158

Sequences

	Sequence		Length	Mass (Da)
	P80195 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 6679E5F1F99CB886	FASTA	153	17,152
	10 20 30 40 50 60 MKFLCVLLLA SLAATSLAIL NKPEDETHLE AQPTDASAQF IRNLQISNED LSKEPSISRE 70 80 90 100 110 120 DLISKEQIVI RSSRQPQSQN PKLPLSILKE KHLRNATLGS EETTEHTPSD ASTTEGKLME 130 140 150 LGHKIMRNLE NTVKETIKYL KSLFSHAFEV VKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of lactophorin from bovine milk." Kuriki H., Motoshima H., Minagawa E., Tsukasaki F., Kanno C. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Milk.
[2]	"Characterization of a bovine mammary gland PP3 cDNA reveals homology with mouse and rat adhesion molecule GlyCAM-1." Johnsen L.B., Soerensen E.S., Petersen T.E., Berglund L. Biochim. Biophys. Acta 1260:116-118(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Black and white Danish dairy cattle.
[3]	"Characterization of a GlyCAM1-like gene (glycosylation-dependent cell adhesion molecule 1) which is highly and specifically expressed in the lactating bovine mammary gland." Groenen M.A., Dijkhof R.J., der Poel J.J. Gene 158:189-195(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lactating mammary gland.
[4]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[5]	"Phosphorylation, glycosylation and amino acid sequence of component PP3 from the proteose peptone fraction of bovine milk." Soerensen E.S., Petersen T.E. J. Dairy Res. 60:535-542(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-153, PHOSPHORYLATION AT SER-47; SER-52; SER-56; SER-58 AND SER-64, GLYCOSYLATION AT THR-34; ASN-95 AND THR-104. Tissue: Milk.
[6]	"Purification and characterization of three proteins isolated from the proteose peptone fraction of bovine milk." Soerensen E.S., Petersen T.E. J. Dairy Res. 60:189-197(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-48 AND 72-91. Tissue: Milk.
[7]	"Complete characterization of posttranslational modification sites in the bovine milk protein PP3 by tandem mass spectrometry with electron capture dissociation as the last stage." Kjeldsen F., Haselmann K.F., Budnik B.A., Sorensen E.S., Zubarev R.A. Anal. Chem. 75:2355-2361(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-47; SER-52; SER-56; SER-58 AND SER-64, GLYCOSYLATION AT THR-34; SER-78; ASN-95 AND THR-104. Tissue: Milk.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26176 mRNA. Translation: BAA05164.1. L36854 mRNA. Translation: AAA79330.1. X79406 mRNA. Translation: CAA55943.1. X83391 Genomic DNA. Translation: CAA58309.1. BC108244 mRNA. Translation: AAI08245.1.
IPI	IPI00716366.
PIR	S52083.
RefSeq	NP_777253.1. NM_174828.2.
UniGene	Bt.4665.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000017854.
PTM databases
GlycoSuiteDB	P80195.
Proteomic databases
PaxDb	P80195.
PRIDE	P80195.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000017854; ENSBTAP00000017854; ENSBTAG00000013417.
GeneID	282430.
KEGG	bta:282430.
Organism-specific databases
CTD	644076.
Phylogenomic databases
eggNOG	NOG86685.
GeneTree	ENSGT00520000060242.
HOGENOM	HOG000290655.
HOVERGEN	HBG106667.
InParanoid	P80195.
KO	K06815.
OMA	EAQDGLR.
OrthoDB	EOG41RPWQ.
Family and domain databases
InterPro	IPR007906. GLYCAM-1. [Graphical view]
PANTHER	PTHR17389. PTHR17389. 1 hit.
Pfam	PF05242. GLYCAM-1. 1 hit. [Graphical view]
ProDom	PD012695. PD012695. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	20806206.

Entry information

Entry name

GLCM1_BOVIN

Accession

Primary (citable) accession number: P80195
Secondary accession number(s): P35451, Q32P62

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program