Reviewed, UniProtKB/Swiss-Prot P80187 (MTRH_METTM)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit H
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit H

Gene names

Name:

mtrH

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step. MtrH catalyzes the transfer of the methyl group from methyl-tetrahydromethanopterin to the corrinoid prosthetic group of mtrA. Ref.3
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01501
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 2/2. HAMAP MF_01501
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH. Ref.1
Sequence similarities	Belongs to the mtrH family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Tetrahydromethanopterin S-methyltransferase subunit H HAMAP MF_01501		PRO_0000147568

Sequences

	Sequence		Length	Mass (Da)
	P80187-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: EB3B44FDD73F2A66	FASTA	310	33,474
	10 20 30 40 50 60 MFRFDKEQIV LDIAGTKIGG QPGEYPTVLA GTIFYGGHSI IEDEKAGVFD KDKAEALIKT 70 80 90 100 110 120 QEEMSDVTGN PHIVQTFGQT PEAIVKYLEF VGDITDAPFF IDSTSGEARI AGAEYASEVG 130 140 150 160 170 180 LEDRAIYNSV NMAADESELE ALKNTKLSAS IVLGFNPMDP TVEGKIGIWE DGAGTIDKGL 190 200 210 220 230 240 LEMAADCGID KYLMDVAVTP LGQGAGVAVR TSFAVKSKWG YPVGSGIHNV PSAWDWLREY 250 260 270 280 290 300 KKEHKEAWPV CDVGSNLIQQ MAGGDFVLYG PIENARMAFP ACAMADIFIS EAAKDIGTEA 310 VEDHPFFKLL

References

[1]	"The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits." Harms U., Weiss D.S., Gaertner P., Linder D., Thauer R.K. Eur. J. Biochem. 228:640-648(1995) [PubMed: 7737157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, SUBUNIT.
[2]	"Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum." Gaertner P., Ecker A., Fischer R., Linder D., Fuchs G., Thauer R.K. Eur. J. Biochem. 213:537-545(1993) [PubMed: 8477726] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30.
[3]	"The energy conserving methyltetrahydromethanopterin:coenzyme M methyltransferase complex from methanogenic archaea: function of the subunit MtrH." Hippler B., Thauer R.K. FEBS Lett. 449:165-168(1999) [PubMed: 10338124] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X84219 Genomic DNA. Translation: CAA59003.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.C.1.1.1. Na+ transporting methyltetrahydromethanopterin:coenzyme M methyltransferase (NaT-MMM) family.
Family and domain databases
HAMAP	MF_01501. [Tree]
InterPro	IPR002856. MtrH_MeTrfase. [Graphical view]
Pfam	PF02007. MtrH. 1 hit. [Graphical view]
ProDom	PD009948. MtrH_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01114. mtrH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRH_METTM

Accession

Primary (citable) accession number: P80187
Secondary accession number(s): Q50775

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 5, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)