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P80177

- MIF_BOVIN

UniProt

P80177 - MIF_BOVIN

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Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
Binding sitei33 – 331SubstrateBy similarity
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
Binding sitei98 – 981SubstrateBy similarity

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. dopachrome isomerase activity Source: UniProtKB
  3. phenylpyruvate tautomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell aging Source: Ensembl
  2. cell proliferation Source: Ensembl
  3. cell surface receptor signaling pathway Source: Ensembl
  4. DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  5. inflammatory response Source: UniProtKB-KW
  6. innate immune response Source: UniProtKB-KW
  7. negative regulation of cell aging Source: Ensembl
  8. negative regulation of cell cycle arrest Source: Ensembl
  9. negative regulation of cellular protein metabolic process Source: Ensembl
  10. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  11. negative regulation of gene expression Source: Ensembl
  12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  13. negative regulation of mature B cell apoptotic process Source: Ensembl
  14. negative regulation of myeloid cell apoptotic process Source: Ensembl
  15. positive regulation of arachidonic acid secretion Source: Ensembl
  16. positive regulation of B cell proliferation Source: Ensembl
  17. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
  18. positive regulation of cytokine secretion Source: Ensembl
  19. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  20. positive regulation of fibroblast proliferation Source: Ensembl
  21. positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
  22. positive regulation of MAP kinase activity Source: Ensembl
  23. positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
  24. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  25. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  26. positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
  27. positive regulation of protein kinase A signaling Source: Ensembl
  28. prostaglandin biosynthetic process Source: Ensembl
  29. protein homotrimerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

SABIO-RKP80177.

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
p12A
Gene namesi
Name:MIF
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 17

Subcellular locationi

Secreted By similarity. Cytoplasm
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens (By similarity).By similarity

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. cytoplasm Source: UniProtKB-KW
  3. extracellular space Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158061Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781N6-acetyllysine; alternateBy similarity
Modified residuei78 – 781N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP80177.

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP80177.
SMRiP80177. Positions 1-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiP80177.
KOiK07253.
OMAiKPERYVM.
OrthoDBiEOG7GXPDN.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80177-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPMFVVNTNV PRASVPDGLL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF
60 70 80 90 100
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLTERLRI SPDRIYINFC
110
DMNAANVGWN GSTFA
Length:115
Mass (Da):12,343
Last modified:January 23, 2007 - v6
Checksum:i750242BFF691975E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 1002FC → YY in AAD38354. (PubMed:10727256)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC102066 mRNA. Translation: AAI02067.1.
AF119571 mRNA. Translation: AAD38354.1.
PIRiS32394.
S48158.
RefSeqiNP_001028780.1. NM_001033608.1.
UniGeneiBt.15528.

Genome annotation databases

EnsembliENSBTAT00000009699; ENSBTAP00000009699; ENSBTAG00000007375.
GeneIDi280858.
KEGGibta:280858.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC102066 mRNA. Translation: AAI02067.1 .
AF119571 mRNA. Translation: AAD38354.1 .
PIRi S32394.
S48158.
RefSeqi NP_001028780.1. NM_001033608.1.
UniGenei Bt.15528.

3D structure databases

ProteinModelPortali P80177.
SMRi P80177. Positions 1-115.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P80177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000009699 ; ENSBTAP00000009699 ; ENSBTAG00000007375 .
GeneIDi 280858.
KEGGi bta:280858.

Organism-specific databases

CTDi 4282.

Phylogenomic databases

eggNOGi NOG08790.
GeneTreei ENSGT00730000111101.
HOGENOMi HOG000112325.
HOVERGENi HBG003240.
InParanoidi P80177.
KOi K07253.
OMAi KPERYVM.
OrthoDBi EOG7GXPDN.
TreeFami TF313853.

Enzyme and pathway databases

SABIO-RK P80177.

Miscellaneous databases

NextBioi 20805002.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
PROSITEi PS01158. MIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  2. "A diversified family of 12-kDa proteins with a high amino acid sequence similarity to macrophage migration-inhibitory factor (MIF)."
    Galat A., Riviere S., Bouet F., Menez A.
    Eur. J. Biochem. 224:417-421(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-115.
    Tissue: Brain.
  3. "Macrophage migration inhibitory factor in the bovine corpus luteum: characterization of steady-state messenger ribonucleic acid and immunohistochemical localization."
    Bove S.E., Petroff M.G., Nishibori M., Pate J.L.
    Biol. Reprod. 62:879-885(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-104.
    Tissue: Corpus luteum.
  4. "Purification of macrophage migration inhibitory factor (MIF) from bovine brain cytosol."
    Galat A., Riviere S., Bouet F.
    FEBS Lett. 319:233-236(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-40.
    Tissue: Brain.

Entry informationi

Entry nameiMIF_BOVIN
AccessioniPrimary (citable) accession number: P80177
Secondary accession number(s): Q3T190, Q9XT46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 116 of the entry and version 6 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3