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Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Proton acceptor; via imino nitrogenBy similarity1
Binding sitei33SubstrateBy similarity1
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei98SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Isomerase
Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-BTA-202733. Cell surface interactions at the vascular wall.
R-BTA-6798695. Neutrophil degranulation.
SABIO-RKiP80177.

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
p12A
Gene namesi
Name:MIF
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 17

Subcellular locationi

  • Secreted By similarity
  • Cytoplasm By similarity

  • Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001580612 – 115Macrophage migration inhibitory factorAdd BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysine; alternateBy similarity1
Modified residuei78N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP80177.
PeptideAtlasiP80177.
PRIDEiP80177.

Expressioni

Gene expression databases

BgeeiENSBTAG00000007375.
ExpressionAtlasiP80177. baseline and differential.

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009699.

Structurei

3D structure databases

ProteinModelPortaliP80177.
SMRiP80177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiKOG1759. Eukaryota.
ENOG41122MF. LUCA.
GeneTreeiENSGT00840000129866.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiP80177.
KOiK07253.
OMAiPDRIYIN.
OrthoDBiEOG091G0ZNJ.
TreeFamiTF313853.

Family and domain databases

InterProiView protein in InterPro
IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiView protein in Pfam
PF01187. MIF. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004816. Macrophage_inhib_fac. 1 hit.
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiView protein in PROSITE
PS01158. MIF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMFVVNTNV PRASVPDGLL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF
60 70 80 90 100
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLTERLRI SPDRIYINFC
110
DMNAANVGWN GSTFA
Length:115
Mass (Da):12,343
Last modified:January 23, 2007 - v6
Checksum:i750242BFF691975E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99 – 100FC → YY in AAD38354 (PubMed:10727256).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102066 mRNA. Translation: AAI02067.1.
AF119571 mRNA. Translation: AAD38354.1.
PIRiS32394.
S48158.
RefSeqiNP_001028780.1. NM_001033608.1.
UniGeneiBt.15528.

Genome annotation databases

EnsembliENSBTAT00000009699; ENSBTAP00000009699; ENSBTAG00000007375.
GeneIDi280858.
KEGGibta:280858.

Similar proteinsi

Entry informationi

Entry nameiMIF_BOVIN
AccessioniPrimary (citable) accession number: P80177
Secondary accession number(s): Q3T190, Q9XT46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 139 of the entry and version 6 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families