P80148 (CISY_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Citrate synthase
EC=2.3.3.1

Gene names

Name:	gltA
Ordered Locus Names:	SSO2589

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.
Enzyme regulation	Allosterically inhibited by NADH.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.
Subunit structure	Homodimer.
Post-translational modification	The N-terminus is blocked by acetylation.
Miscellaneous	Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Sequence similarities	Belongs to the citrate synthase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Molecular function	Transferase
PTM	Acetylation
Technical term	3D-structure Allosteric enzyme Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	citrate (Si)-synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 377

377

Citrate synthase

PRO_0000169977

Sites

Active site

258

1

By similarity

Active site

313

1

By similarity

Experimental info

Sequence conflict

10

1

N → D AA sequence Ref.3

Sequence conflict

12

1

I → Y AA sequence Ref.3

Sequence conflict

15

1

V → S AA sequence Ref.3

Sequence conflict

17

1

N → S AA sequence Ref.3

Sequence conflict

20

1

F → Y AA sequence Ref.3

Sequence conflict

24 – 25

2

EK → VN AA sequence Ref.3

Sequence conflict

27

1

I → V AA sequence Ref.3

Sequence conflict

57

1

P → R in AAB09594. Ref.1

Secondary structure

1

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377

Helix Strand Turn

Details...

Helix

6 – 8

3

Beta strand

12 – 22

11

Turn

23 – 26

4

Beta strand

27 – 30

4

Helix

35 – 41

7

Helix

44 – 53

10

Helix

59 – 70

12

Helix

77 – 85

9

Helix

92 – 106

15

Beta strand

112 – 115

4

Helix

116 – 138

23

Helix

152 – 161

10

Helix

167 – 180

14

Helix

187 – 197

11

Helix

202 – 213

12

Turn

216 – 220

5

Helix

221 – 232

12

Helix

235 – 237

3

Helix

238 – 245

8

Turn

246 – 249

4

Helix

266 – 279

14

Helix

283 – 303

21

Turn

304 – 307

4

Turn

312 – 315

4

Helix

316 – 323

8

Helix

327 – 329

3

Helix

330 – 352

23

Beta strand

360 – 363

4

Sequences

	Sequence		Length	Mass (Da)
	P80148 [UniParc]. Last modified June 1, 2001. Version 3. Checksum: E3036E232F134F11	FASTA	377	42,752
	10 20 30 40 50 60 MSVVSKGLEN VIIKVTNLTF IDGEKGILRY RGYNIEDLVN YGSYEETIYL MLYGKLPTKK 70 80 90 100 110 120 ELNDLKAKLN EEYEVPQEVL DTIYLMPKEA DAIGLLEVGT AALASIDKNF KWKENDKEKA 130 140 150 160 170 180 ISIIAKMATL VANVYRRKEG NKPRIPEPSD SFAKSFLLAS FAREPTTDEI NAMDKALILY 190 200 210 220 230 240 TDHEVPASTT AALVAASTLS DMYSSLTAAL AALKGPLHGG AAEEAFKQFI EIGDPNRVQN 250 260 270 280 290 300 WFNDKVVNQK NRLMGFGHRV YKTYDPRAKI FKKLALTLIE RNADARRYFE IAQKLEELGI 310 320 330 340 350 360 KQFSSKGIYP NTDFYSGIVF YALGFPVYMF TALFALSRTL GWLAHIIEYV EEQHRLIRPR 370 ALYVGPEYQE YVSIDKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sulfolobus solfataricus citrate synthase." Connaris H., Danson M.J., Hough D.W. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"Conversion, by limited proteolysis, of an archaebacterial citrate synthase into essentially a citryl-CoA hydrolase." Lill U., Lefrank S., Henschen A., Eggerer H. Eur. J. Biochem. 208:459-466(1992) [PubMed: 1521537] [Abstract] Cited for: PROTEIN SEQUENCE OF 7-30, ACETYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U70879 Genomic DNA. Translation: AAB09594.1.
AE006641 Genomic DNA. Translation: AAK42713.1.

PIR

B90432.

RefSeq

NP_343923.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O7X	X-ray	2.70	A/B/C/D	1-377	[»]

ProteinModelPortal

P80148.

SMR

P80148. Positions 3-372.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1454040.

GenomeReviews

Gene locus SSO2589 in contig AE006641_GR.

KEGG

sso:SSO2589.

NMPDR

fig|273057.1.peg.2346.

Phylogenomic databases

HOGENOM

HBG649489.

OMA

NDIEHRE.

ProtClustDB

PRK14037.

Enzyme and pathway databases

BioCyc

SSOL273057:SSO2589-MONOMER.

Family and domain databases

InterPro

IPR011278. 2-MeCitrate/Citrate_synth_I.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]

Gene3D

G3DSA:1.10.580.10. Citrate_synthase_lrg_a-sub. 1 hit.
G3DSA:1.10.230.10. Citrate_synthase_sm_a-sub. 1 hit.

KO

K01647.

PANTHER

PTHR11739. Citrate_synth. 1 hit.

Pfam

PF00285. Citrate_synt. 1 hit.
[Graphical view]

PIRSF

PIRSF001369. Citrate_synth. 1 hit.

PRINTS

PR00143. CITRTSNTHASE.

SUPFAM

SSF48256. Citrate_synthase_core. 1 hit.

TIGRFAMs

TIGR01800. Cit_synth_II. 1 hit.

PROSITE

PS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CISY_SULSO

Accession

Primary (citable) accession number: P80148
Secondary accession number(s): P77979

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program