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P80147

- GABT_PIG

UniProt

P80147 - GABT_PIG

Protein

4-aminobutyrate aminotransferase, mitochondrial

Gene

ABAT

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

    Catalytic activityi

    4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
    (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    GO - Molecular functioni

    1. (S)-3-amino-2-methylpropionate transaminase activity Source: UniProtKB-EC
    2. 4-aminobutyrate transaminase activity Source: UniProtKB-EC
    3. protein homodimerization activity Source: UniProtKB
    4. pyridoxal phosphate binding Source: UniProtKB
    5. succinate-semialdehyde dehydrogenase binding Source: UniProtKB

    GO - Biological processi

    1. behavioral response to cocaine Source: UniProtKB
    2. gamma-aminobutyric acid catabolic process Source: UniProtKB
    3. neurotransmitter catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Neurotransmitter degradation

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_219022. Degradation of GABA.
    SABIO-RKP80147.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-aminobutyrate aminotransferase, mitochondrial (EC:2.6.1.19)
    Alternative name(s):
    (S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
    GABA aminotransferase
    Short name:
    GABA-AT
    Gamma-amino-N-butyrate transaminase
    Short name:
    GABA transaminase
    Short name:
    GABA-T
    L-AIBAT
    Gene namesi
    Name:ABAT
    Synonyms:GABAT
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. 4-aminobutyrate transaminase complex Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. mitochondrial matrix Source: UniProtKB
    4. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828Mitochondrion1 PublicationAdd
    BLAST
    Chaini29 – 5004724-aminobutyrate aminotransferase, mitochondrialPRO_0000001251Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi163 ↔ 166
    Modified residuei231 – 2311N6-succinyllysineBy similarity
    Modified residuei252 – 2521N6-acetyllysine; alternateBy similarity
    Modified residuei252 – 2521N6-succinyllysine; alternateBy similarity
    Modified residuei279 – 2791N6-acetyllysineBy similarity
    Modified residuei318 – 3181N6-acetyllysineBy similarity
    Disulfide bondi321 – 321InterchainBy similarity
    Modified residuei357 – 3571N6-(pyridoxal phosphate)lysine
    Modified residuei413 – 4131N6-acetyllysine; alternateBy similarity
    Modified residuei413 – 4131N6-succinyllysine; alternateBy similarity
    Modified residuei452 – 4521N6-acetyllysineBy similarity
    Modified residuei470 – 4701N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond

    Proteomic databases

    PaxDbiP80147.
    PRIDEiP80147.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    BioGridi1149627. 1 interaction.
    STRINGi9823.ENSSSCP00000008445.

    Structurei

    Secondary structure

    1
    500
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi53 – 6513
    Helixi77 – 793
    Beta strandi84 – 874
    Beta strandi92 – 976
    Helixi98 – 1014
    Helixi110 – 1178
    Helixi119 – 1213
    Helixi122 – 1265
    Turni131 – 1333
    Helixi139 – 1457
    Helixi147 – 1504
    Beta strandi157 – 1637
    Helixi164 – 18421
    Helixi191 – 1988
    Turni202 – 2043
    Beta strandi209 – 2135
    Helixi222 – 2276
    Helixi232 – 2354
    Helixi255 – 2573
    Helixi259 – 28224
    Beta strandi287 – 2926
    Beta strandi294 – 2963
    Turni298 – 3003
    Helixi306 – 31813
    Beta strandi322 – 3265
    Turni328 – 3358
    Beta strandi336 – 3394
    Helixi340 – 3445
    Beta strandi351 – 3555
    Helixi357 – 3593
    Beta strandi360 – 3667
    Helixi368 – 3703
    Beta strandi381 – 3844
    Helixi386 – 40116
    Helixi404 – 42522
    Turni427 – 4293
    Beta strandi431 – 4366
    Beta strandi439 – 4435
    Helixi447 – 45913
    Beta strandi465 – 4673
    Turni468 – 4703
    Beta strandi471 – 4744
    Helixi482 – 49716

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OHVX-ray2.30A/B/C/D29-500[»]
    1OHWX-ray2.30A/B/C/D29-500[»]
    1OHYX-ray2.80A/B/C/D29-500[»]
    ProteinModelPortaliP80147.
    SMRiP80147. Positions 39-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP80147.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0160.
    HOGENOMiHOG000020208.
    HOVERGENiHBG000634.
    KOiK13524.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProiIPR004631. 4NH2But_aminotransferase_euk.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PTHR11986:SF6. PTHR11986:SF6. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80147-1 [UniParc]FASTAAdd to Basket

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    MASVLLTRRL ACSFRHNHRL LVPGWRHISQ AAAKVDVEFD YDGPLMKTEV    50
    PGPRSRELMK QLNIIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI 100
    SSIPIGYSHP ALVKLVQQPQ NVSTFINRPA LGILPPENFV EKLRESLLSV 150
    APKGMSQLIT MACGSCSNEN AFKTIFMWYR SKERGQSAFS KEELETCMIN 200
    QAPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR 250
    LKYPLEEFVK ENQQEEARCL EEVEDLIVKY RKKKKTVAGI IVEPIQSEGG 300
    DNHASDDFFR KLRDISRKHG CAFLVDEVQT GGGSTGKFWA HEHWGLDDPA 350
    DVMTFSKKMM TGGFFHKEEF RPNAPYRIFN TWLGDPSKNL LLAEVINIIK 400
    REDLLSNAAH AGKVLLTGLL DLQARYPQFI SRVRGRGTFC SFDTPDESIR 450
    NKLISIARNK GVMLGGCGDK SIRFRPTLVF RDHHAHLFLN IFSDILADFK 500
    Length:500
    Mass (Da):56,620
    Last modified:December 1, 1992 - v2
    Checksum:iBFD0B80846CEF5B7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti454 – 4541I → N in AAA96981. (PubMed:1559966)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84802 mRNA. Translation: AAA96981.1.
    RefSeqiNP_999428.1. NM_214263.1.
    UniGeneiSsc.16251.

    Genome annotation databases

    GeneIDi397500.
    KEGGissc:397500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84802 mRNA. Translation: AAA96981.1 .
    RefSeqi NP_999428.1. NM_214263.1.
    UniGenei Ssc.16251.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OHV X-ray 2.30 A/B/C/D 29-500 [» ]
    1OHW X-ray 2.30 A/B/C/D 29-500 [» ]
    1OHY X-ray 2.80 A/B/C/D 29-500 [» ]
    ProteinModelPortali P80147.
    SMRi P80147. Positions 39-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1149627. 1 interaction.
    STRINGi 9823.ENSSSCP00000008445.

    Chemistry

    BindingDBi P80147.
    ChEMBLi CHEMBL2266.

    Proteomic databases

    PaxDbi P80147.
    PRIDEi P80147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397500.
    KEGGi ssc:397500.

    Organism-specific databases

    CTDi 18.

    Phylogenomic databases

    eggNOGi COG0160.
    HOGENOMi HOG000020208.
    HOVERGENi HBG000634.
    KOi K13524.

    Enzyme and pathway databases

    Reactomei REACT_219022. Degradation of GABA.
    SABIO-RK P80147.

    Miscellaneous databases

    EvolutionaryTracei P80147.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProi IPR004631. 4NH2But_aminotransferase_euk.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    PTHR11986:SF6. PTHR11986:SF6. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00699. GABAtrns_euk. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Brain 4-aminobutyrate aminotransferase. Isolation and sequence of a cDNA encoding the enzyme."
      Kwon O.S., Park J., Churchich J.E.
      J. Biol. Chem. 267:7215-7216(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Protein structure of pig liver 4-aminobutyrate aminotransferase and comparison with a cDNA-deduced sequence."
      de Biase D., Maras B., Bossa F., Barra D., John R.A.
      Eur. J. Biochem. 208:351-357(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-500.
      Tissue: Liver.
    3. "Crystal structure of GABA-aminotransferase, a target for antiepileptic drug therapy."
      Storici P., Capitani G., De Biase D., Moser M., John R.A., Jansonius J.N., Schirmer T.
      Biochemistry 38:8628-8634(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
      Tissue: Liver.

    Entry informationi

    Entry nameiGABT_PIG
    AccessioniPrimary (citable) accession number: P80147
    Secondary accession number(s): P27820
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3