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Protein

Phosphatidylinositol-glycan-specific phospholipase D

Gene

GPLD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.1 Publication

Catalytic activityi

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.4.50. 908.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan-specific phospholipase D (EC:3.1.4.50)
Short name:
PI-G PLD
Alternative name(s):
Glycoprotein phospholipase D
Glycosyl-phosphatidylinositol-specific phospholipase D
Short name:
GPI-PLD
Short name:
GPI-specific phospholipase D
Gene namesi
Name:GPLD1
Synonyms:PIGPLD
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi834Y → A: Severe loss of enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000002205324 – 839Phosphatidylinositol-glycan-specific phospholipase DAdd BLAST816

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi500N-linked (GlcNAc...)Sequence analysis1
Glycosylationi590N-linked (GlcNAc...)Sequence analysis1
Glycosylationi658N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP80109.
PRIDEiP80109.

PTM databases

iPTMnetiP80109.

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006557.

Structurei

3D structure databases

ProteinModelPortaliP80109.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati365 – 427FG-GAP 1PROSITE-ProRule annotationAdd BLAST63
Repeati434 – 496FG-GAP 2PROSITE-ProRule annotationAdd BLAST63
Repeati498 – 558FG-GAP 3PROSITE-ProRule annotationAdd BLAST61
Repeati562 – 622FG-GAP 4PROSITE-ProRule annotationAdd BLAST61
Repeati632 – 692FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati703 – 769FG-GAP 6PROSITE-ProRule annotationAdd BLAST67
Repeati787 – 839FG-GAP 7PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the GPLD1 family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IPB3. Eukaryota.
ENOG4111ZDA. LUCA.
HOGENOMiHOG000007655.
HOVERGENiHBG008185.
InParanoidiP80109.
KOiK01127.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00718. PHPHLIPASED.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFRFWSGL LMLLGFLCPR SSPCGISTHI EIGHRALEFL HLQDGSINYK
60 70 80 90 100
ELLLRHQDAY QAGSVFPDSF YPSICERGQF HDVSESTHWT PFLNASVHYI
110 120 130 140 150
RKNYPLPWDE DTEKLVAFLF GITSHMVADV NWHSLGIEQG FLRTMAAIDF
160 170 180 190 200
HNSYPEAHPA GDFGGDVLSQ FEFKFNYLSR HWYVPAEDLL GIYRELYGRI
210 220 230 240 250
VITKKAIVDC SYLQFLEMYA EMLAISKLYP TYSVKSPFLV EQFQEYFLGG
260 270 280 290 300
LEDMAFWSTN IYHLTSYMLK NGTSNCNLPE NPLFITCGGQ QNNTHGSKVQ
310 320 330 340 350
KNGFHKNVTA ALTKNIGKHI NYTKRGVFFS VDSWTMDSLS FMYKSLERSI
360 370 380 390 400
REMFIGSSQP LTHVSSPAAS YYLSFPYTRL GWAMTSADLN QDGYGDLVVG
410 420 430 440 450
APGYSHPGRI HVGRVYLIYG NDLGLPRIDL DLDKEAHGIL EGFQPSGRFG
460 470 480 490 500
SAVAVLDFNV DGVPDLAVGA PSVGSEKLTY TGAVYVYFGS KQGQLSSSPN
510 520 530 540 550
VTISCQDTYC NLGWTLLAAD VNGDSEPDLV IGSPFAPGGG KQKGIVAAFY
560 570 580 590 600
SGSSYSSREK LNVEAANWMV KGEEDFAWLG YSLHGVNVNN RTLLLAGSPT
610 620 630 640 650
WKDTSSQGHL FRTRDEKQSP GRVYGYFPPI CQSWFTISGD KAMGKLGTSL
660 670 680 690 700
SSGHVMVNGT RTQVLLVGAP TQDVVSKVSF LTMTLHQGGS TRMYELTPDS
710 720 730 740 750
QPSLLSTFSG NRRFSRFGGV LHLSDLDNDG LDEIIVAAPL RITDATAGLM
760 770 780 790 800
GEEDGRVYVF NGKQITVGDV TGKCKSWVTP CPEEKAQYVL ISPEAGSRFG
810 820 830
SSVITVRSKE KNQVIIAAGR SSLGARLSGV LHIYRLGQD
Length:839
Mass (Da):92,602
Last modified:July 1, 1993 - v1
Checksum:iF9BFF8A00226BF40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181H → F AA sequence (PubMed:2170394).Curated1
Sequence conflicti184V → L AA sequence (PubMed:2170394).Curated1
Sequence conflicti235K → R AA sequence (PubMed:2170394).Curated1
Sequence conflicti634W → P AA sequence (PubMed:2170394).Curated1
Sequence conflicti777W → N AA sequence (PubMed:2170394).Curated1
Sequence conflicti780P → S AA sequence (PubMed:2170394).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60804 mRNA. Translation: AAA30721.1.
PIRiA56337.
RefSeqiNP_777241.1. NM_174816.2.
UniGeneiBt.452.

Genome annotation databases

GeneIDi287025.
KEGGibta:287025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60804 mRNA. Translation: AAA30721.1.
PIRiA56337.
RefSeqiNP_777241.1. NM_174816.2.
UniGeneiBt.452.

3D structure databases

ProteinModelPortaliP80109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006557.

PTM databases

iPTMnetiP80109.

Proteomic databases

PaxDbiP80109.
PRIDEiP80109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287025.
KEGGibta:287025.

Organism-specific databases

CTDi2822.

Phylogenomic databases

eggNOGiENOG410IPB3. Eukaryota.
ENOG4111ZDA. LUCA.
HOGENOMiHOG000007655.
HOVERGENiHBG008185.
InParanoidiP80109.
KOiK01127.

Enzyme and pathway databases

BRENDAi3.1.4.50. 908.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00718. PHPHLIPASED.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHLD_BOVIN
AccessioniPrimary (citable) accession number: P80109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.