Reviewed, UniProtKB/Swiss-Prot P80109 (PHLD_BOVIN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-glycan-specific phospholipase D
      Short name=PI-G PLD
    EC=3.1.4.50
Alternative name(s):
    Glycoprotein phospholipase D
    Glycosyl-phosphatidylinositol-specific phospholipase D
      Short name=GPI-specific phospholipase D
      Short name=GPI-PLD

Gene names

Name:	GPLD1
Synonyms:	PIGPLD

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	839 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Ref.4
Catalytic activity	6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.
Subunit structure	Monomer Potential.
Subcellular location	Secreted. Note: Associated with the High-Density Lipoproteins (HDL). Ref.4
Post-translational modification	Glycosylated. Ref.4 Ref.2
Sequence similarities	Belongs to the GPLD1 family. Contains 4 FG-GAP repeats.

Ontologies

Keywords
Cellular component	HDL Secreted
Domain	Repeat Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycosylphosphatidylinositol phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.3
	Chain	24 – 839	816	Phosphatidylinositol-glycan-specific phospholipase D		PRO_0000022053
Regions
	Repeat	380 – 418	39	FG-GAP 1
	Repeat	449 – 487	39	FG-GAP 2
	Repeat	512 – 549	38	FG-GAP 3
	Repeat	717 – 749	33	FG-GAP 4
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	321	1	N-linked (GlcNAc...) Potential
	Glycosylation	500	1	N-linked (GlcNAc...) Potential
	Glycosylation	590	1	N-linked (GlcNAc...) Potential
	Glycosylation	658	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	834	1	Y → A: Severe loss of enzymatic activity. Ref.4
	Sequence conflict	181	1	H → F AA sequence Ref.2
	Sequence conflict	184	1	V → L AA sequence Ref.2
	Sequence conflict	235	1	K → R AA sequence Ref.2
	Sequence conflict	634	1	W → P AA sequence Ref.2
	Sequence conflict	777	1	W → N AA sequence Ref.2
	Sequence conflict	780	1	P → S AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80109-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: F9BFF8A00226BF40	FASTA	839	92,602
	10 20 30 40 50 60 MSAFRFWSGL LMLLGFLCPR SSPCGISTHI EIGHRALEFL HLQDGSINYK ELLLRHQDAY 70 80 90 100 110 120 QAGSVFPDSF YPSICERGQF HDVSESTHWT PFLNASVHYI RKNYPLPWDE DTEKLVAFLF 130 140 150 160 170 180 GITSHMVADV NWHSLGIEQG FLRTMAAIDF HNSYPEAHPA GDFGGDVLSQ FEFKFNYLSR 190 200 210 220 230 240 HWYVPAEDLL GIYRELYGRI VITKKAIVDC SYLQFLEMYA EMLAISKLYP TYSVKSPFLV 250 260 270 280 290 300 EQFQEYFLGG LEDMAFWSTN IYHLTSYMLK NGTSNCNLPE NPLFITCGGQ QNNTHGSKVQ 310 320 330 340 350 360 KNGFHKNVTA ALTKNIGKHI NYTKRGVFFS VDSWTMDSLS FMYKSLERSI REMFIGSSQP 370 380 390 400 410 420 LTHVSSPAAS YYLSFPYTRL GWAMTSADLN QDGYGDLVVG APGYSHPGRI HVGRVYLIYG 430 440 450 460 470 480 NDLGLPRIDL DLDKEAHGIL EGFQPSGRFG SAVAVLDFNV DGVPDLAVGA PSVGSEKLTY 490 500 510 520 530 540 TGAVYVYFGS KQGQLSSSPN VTISCQDTYC NLGWTLLAAD VNGDSEPDLV IGSPFAPGGG 550 560 570 580 590 600 KQKGIVAAFY SGSSYSSREK LNVEAANWMV KGEEDFAWLG YSLHGVNVNN RTLLLAGSPT 610 620 630 640 650 660 WKDTSSQGHL FRTRDEKQSP GRVYGYFPPI CQSWFTISGD KAMGKLGTSL SSGHVMVNGT 670 680 690 700 710 720 RTQVLLVGAP TQDVVSKVSF LTMTLHQGGS TRMYELTPDS QPSLLSTFSG NRRFSRFGGV 730 740 750 760 770 780 LHLSDLDNDG LDEIIVAAPL RITDATAGLM GEEDGRVYVF NGKQITVGDV TGKCKSWVTP 790 800 810 820 830 CPEEKAQYVL ISPEAGSRFG SSVITVRSKE KNQVIIAAGR SSLGARLSGV LHIYRLGQD

References

[1]	"Primary structure and functional activity of a phosphatidylinositol-glycan-specific phospholipase D." Scallon B.J., Fung W.-J.C., Tsang T.C., Li S., Kado-Fong H., Huang K.-S., Kochan J.P. Science 252:446-448(1991) [PubMed: 2017684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Purification and characterization of glycosyl-phosphatidylinositol-specific phospholipase D." Huang K.S., Li S., Fung W.J., Hulmes J.D., Reik L., Pan Y.C., Low M.G. J. Biol. Chem. 265:17738-17745(1990) [PubMed: 2170394] [Abstract] Cited for: PROTEIN SEQUENCE OF 36-50; 56-77; 126-138; 181-185; 235-261; 380-408; 449-477; 623-636; 678-692 AND 776-807, GLYCOSYLATION. Tissue: Serum.
[3]	"Phosphatidylinositol-glycan-specific phospholipase D is an amphiphilic glycoprotein that in serum is associated with high-density lipoproteins." Hoener M.C., Brodbeck U. Eur. J. Biochem. 206:747-757(1992) [PubMed: 1606959] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-50; 126-138; 145-164; 353-367; 569-579; 693-708 AND 750-773. Tissue: Serum.
[4]	"The C-terminus of glycosylphosphatidylinositol-specific phospholipase D is essential for biological activity." Stadelmann B., Buetikofer P., Koenig A., Brodbeck U. Biochim. Biophys. Acta 1355:107-113(1997) [PubMed: 9042330] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TYR-834, SUBCELLULAR LOCATION, GLYCOSYLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60804 mRNA. Translation: AAA30721.1.
IPI	IPI00701859.
PIR	A56337.
RefSeq	NP_777241.1.
UniGene	Bt.452
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P80109.
Genome annotation databases
Ensembl	ENSBTAT00000006557; ENSBTAP00000006557; ENSBTAG00000004982; Bos taurus. [Genome view]
GeneID	287025.
KEGG	bta:287025.
Organism-specific databases
CTD	287025.
Phylogenomic databases
HOVERGEN	P80109.
Enzyme and pathway databases
BRENDA	3.1.4.50. 251.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR001028. Gprt_PlipaseD. IPR013519. Int_alpha_beta-p. [Graphical view]
PANTHER	PTHR23221:SF2. Gprt_PlipaseD. 1 hit.
Pfam	PF01839. FG-GAP. 4 hits. [Graphical view]
PRINTS	PR00718. PHPHLIPASED.
SMART	SM00191. Int_alpha. 5 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHLD_BOVIN

Accession

Primary (citable) accession number: P80109

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 1, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)