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Reviewed, UniProtKB/Swiss-Prot P80108 (PHLD_HUMAN)

Last modified November 25, 2008. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphatidylinositol-glycan-specific phospholipase D
      Short name=PI-G PLD
    EC=3.1.4.50
Alternative name(s):
    Glycoprotein phospholipase D
    Glycosyl-phosphatidylinositol-specific phospholipase D
Gene names
Name: GPLD1
Synonyms: PIGPLD1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.

Catalytic activity

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H(2)O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.

Subcellular location

Secreted.

Sequence similarities

Contains 4 FG-GAP repeats.

Sequence caution

The sequence AAA36444.1 differs from that shown. Reason: Miscellaneous discrepancy. This sequence has numerous of conflicts with the human genome.

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Ontologies

Keywords

   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Signal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Molecular functionglycosylphosphatidylinositol phospholipase D activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P80108-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P80108-2)

The sequence of this isoform differs from the canonical sequence as follows:
     165-176: GDVLSQFEFNFN → TVYLHLLNFLVV
     177-840: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323
Chain24 – 840817Phosphatidylinositol-glycan-specific phospholipase D
PRO_0000022047

Regions

Repeat381 – 41939FG-GAP 1
Repeat450 – 48839FG-GAP 2
Repeat513 – 55038FG-GAP 3
Repeat718 – 75033FG-GAP 4

Amino acid modifications

Glycosylation941N-linked (GlcNAc...)
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation2921N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3211N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Glycosylation5681N-linked (GlcNAc...) Potential
Glycosylation5911N-linked (GlcNAc...) Potential
Glycosylation6041N-linked (GlcNAc...) Potential
Glycosylation6591N-linked (GlcNAc...) Probable

Natural variations

Alternative sequence165 – 17612GDVLS…EFNFN → TVYLHLLNFLVV in isoform 2.
VSP_023261
Alternative sequence177 – 840664Missing in isoform 2.
VSP_023262
Natural variant171L → V: dbSNP rs2235501.
VAR_030743
Natural variant301V → I: dbSNP rs1126617.
VAR_030744
Natural variant2751D → E: dbSNP rs17300770.
VAR_030745
Natural variant3961G → S: dbSNP rs6924628.
VAR_030746
Natural variant4611V → M: dbSNP rs1062505.
VAR_030747
Natural variant6941M → V: dbSNP rs1042303.
VAR_030748
Natural variant6981T → I: dbSNP rs1772256.
VAR_030749

Experimental info

Sequence conflict12 – 154IMLG → MIVMA in AAA36444. Ref.1
Sequence conflict231P → S in AAA36444. Ref.1
Sequence conflict411Q → H in AAA36444. Ref.1
Sequence conflict461R → H in AAA36444. Ref.1
Sequence conflict501R → K in AAA36444. Ref.1
Sequence conflict641I → T in AAA36444. Ref.1
Sequence conflict741I → L in AAA36444. Ref.1
Sequence conflict1271A → V in AAA36444. Ref.1
Sequence conflict1371L → I in AAA36444. Ref.1
Sequence conflict2001K → E in AAA36444. Ref.1
Sequence conflict2311T → S in AAA36444. Ref.1
Sequence conflict2661S → I in AAA36445. Ref.1
Sequence conflict2771N → S in AAA36444. Ref.1
Sequence conflict3101T → S in AAA36444. Ref.1
Sequence conflict315 – 3162SV → NI in AAA36444. Ref.1
Sequence conflict3501I → V in AAA36444. Ref.1
Sequence conflict3651V → I in AAA36444. Ref.1
Sequence conflict3951H → Y in AAA36444. Ref.1
Sequence conflict4101H → R in AAA36444. Ref.1
Sequence conflict4231D → E in AAA36444. Ref.1
Sequence conflict4391R → G in AAA36444. Ref.1
Sequence conflict4561V → M in AAA36444. Ref.1
Sequence conflict4951G → R in AAA36444. Ref.1
Sequence conflict531 – 5344VIGS → MLGT AA sequence Ref.6
Sequence conflict5581D → N in AAA36444. Ref.1
Sequence conflict5781S → A in AAA36444. Ref.1
Sequence conflict6101H → R in AAA36444. Ref.1
Sequence conflict6321G → S in AAA36444. Ref.1
Sequence conflict6391S → V in AAA36444. Ref.1
Sequence conflict6631K → T in AAA36444. Ref.1
Sequence conflict6731Y → R in AAA36444. Ref.1
Sequence conflict6791V → M in AAA36444. Ref.1
Sequence conflict6841V → M in AAA36444. Ref.1
Sequence conflict7011A → L in AAA36444. Ref.1
Sequence conflict7041L → P in AAA36444. Ref.1
Sequence conflict7321L → V in AAA36444. Ref.1
Sequence conflict7371M → V in AAA36444. Ref.1
Sequence conflict7791I → M in AAA36444. Ref.1
Sequence conflict8371L → F in AAA36444. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 3.
Checksum: 11369BAFEC3D6D38

FASTA84092,336
        10         20         30         40         50         60 
MSAFRLWPGL LIMLGSLCHR GSPCGLSTHV EIGHRALEFL QLHNGRVNYR ELLLEHQDAY 

        70         80         90        100        110        120 
QAGIVFPDCF YPSICKGGKF HDVSESTHWT PFLNASVHYI RENYPLPWEK DTEKLVAFLF 

       130        140        150        160        170        180 
GITSHMAADV SWHSLGLEQG FLRTMGAIDF HGSYSEAHSA GDFGGDVLSQ FEFNFNYLAR 

       190        200        210        220        230        240 
RWYVPVKDLL GIYEKLYGRK VITENVIVDC SHIQFLEMYG EMLAVSKLYP TYSTKSPFLV 

       250        260        270        280        290        300 
EQFQEYFLGG LDDMAFWSTN IYHLTSFMLE NGTSDCNLPE NPLFIACGGQ QNHTQGSKMQ 

       310        320        330        340        350        360 
KNDFHRNLTT SLTESVDRNI NYTERGVFFS VNSWTPDSMS FIYKALERNI RTMFIGGSQL 

       370        380        390        400        410        420 
SQKHVSSPLA SYFLSFPYAR LGWAMTSADL NQDGHGDLVV GAPGYSRPGH IHIGRVYLIY 

       430        440        450        460        470        480 
GNDLGLPPVD LDLDKEAHRI LEGFQPSGRF GSALAVLDFN VDGVPDLAVG APSVGSEQLT 

       490        500        510        520        530        540 
YKGAVYVYFG SKQGGMSSSP NITISCQDIY CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG 

       550        560        570        580        590        600 
GKQKGIVAAF YSGPSLSDKE KLNVEAANWT VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP 

       610        620        630        640        650        660 
TWKNASRLGH LLHIRDEKKS LGRVYGYFPP NGQSWFTISG DKAMGKLGTS LSSGHVLMNG 

       670        680        690        700        710        720 
TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG ATRMYALTSD AQPLLLSTFS GDRRFSRFGG 

       730        740        750        760        770        780 
VLHLSDLDDD GLDEIIMAAP LRIADVTSGL IGGEDGRVYV YNGKETTLGD MTGKCKSWIT 

       790        800        810        820        830        840 
PCPEEKAQYV LISPEASSRF GSSLITVRSK AKNQVVIAAG RSSLGARLSG ALHVYSLGSD

« Hide

Isoform 2 [UniParc].

Checksum: B71070D7DD58234B
Show »

17619,865

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References

« Hide 'large scale' references
[1]"Isolation and expression of two human glycosylphosphatidylinositol phospholipase D (GPI-PLD) cDNAs."
Tsang T.C., Fung W.-J.C., Levine J., Metz C.N., Davitz M.A., Burns D.K., Huang K.-S., Kochan J.P.
FASEB J. 6:A1922-A1922(1992)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ILE-30; MET-461 AND ILE-698.
Tissue: Liver and Pancreas.
[2]"Structure and expression of the human glycosylphosphatidylinositol phospholipase D1 (GPLD1) gene."
Schofield J.N., Rademacher T.W.
Biochim. Biophys. Acta 1494:189-194(2000) [PubMed: 11072085] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 384-840, VARIANTS ILE-30; VAL-694 AND ILE-698.
Tissue: Liver.
[3]"Preliminary study of the gene structure of human glycosylphosphatidylinositol specific phospholipase D."
Tang J.H., Gu S.L., Zhang X.J.
Hunan Yi Ke Da Xue Xue Bao 26:96-97(2001)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ILE-698.
Tissue: Bone marrow.
[4]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed: 14574404] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [