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Protein

Phosphatidylinositol-glycan-specific phospholipase D

Gene

GPLD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.

Catalytic activityi

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.

GO - Molecular functioni

  • glycosylphosphatidylinositol phospholipase D activity Source: UniProtKB
  • phospholipase D activity Source: UniProtKB
  • sodium channel regulator activity Source: UniProtKB

GO - Biological processi

  • cell migration involved in sprouting angiogenesis Source: UniProtKB
  • cellular response to calcium ion Source: UniProtKB
  • cellular response to cholesterol Source: UniProtKB
  • cellular response to drug Source: UniProtKB
  • cellular response to insulin stimulus Source: UniProtKB
  • cellular response to pH Source: UniProtKB
  • cellular response to triglyceride Source: UniProtKB
  • chondrocyte differentiation Source: UniProtKB
  • complement receptor mediated signaling pathway Source: UniProtKB
  • GPI anchor release Source: UniProtKB
  • hematopoietic stem cell migration Source: UniProtKB
  • hematopoietic stem cell migration to bone marrow Source: UniProtKB
  • insulin receptor signaling pathway Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of triglyceride catabolic process Source: UniProtKB
  • ossification Source: UniProtKB
  • phosphatidylcholine metabolic process Source: UniProtKB
  • positive regulation of alkaline phosphatase activity Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of cytolysis Source: UniProtKB
  • positive regulation of endothelial cell migration Source: UniProtKB
  • positive regulation of glucose metabolic process Source: UniProtKB
  • positive regulation of high-density lipoprotein particle clearance Source: UniProtKB
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • positive regulation of membrane protein ectodomain proteolysis Source: UniProtKB
  • positive regulation of secretion Source: UniProtKB
  • positive regulation of triglyceride biosynthetic process Source: UniProtKB
  • regulation of cellular response to insulin stimulus Source: UniProtKB
  • response to glucose Source: UniProtKB
  • transepithelial transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciZFISH:HS03549-MONOMER.
BRENDAi3.1.4.50. 2681.
SignaLinkiP80108.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan-specific phospholipase D (EC:3.1.4.50)
Short name:
PI-G PLD
Alternative name(s):
Glycoprotein phospholipase D
Glycosyl-phosphatidylinositol-specific phospholipase D
Short name:
GPI-PLD
Short name:
GPI-specific phospholipase D
Gene namesi
Name:GPLD1
Synonyms:PIGPLD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:4459. GPLD1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • intracellular Source: UniProtKB
  • intracellular membrane-bounded organelle Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • proteinaceous extracellular matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi2822.
OpenTargetsiENSG00000112293.
PharmGKBiPA28842.

Polymorphism and mutation databases

BioMutaiGPLD1.
DMDMi126302583.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000002204724 – 840Phosphatidylinositol-glycan-specific phospholipase DAdd BLAST817

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...)1 Publication1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1
Glycosylationi568N-linked (GlcNAc...)Sequence analysis1
Glycosylationi591N-linked (GlcNAc...)Sequence analysis1
Glycosylationi604N-linked (GlcNAc...)Sequence analysis1
Glycosylationi659N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP80108.
PeptideAtlasiP80108.
PRIDEiP80108.

PTM databases

iPTMnetiP80108.
PhosphoSitePlusiP80108.

Expressioni

Gene expression databases

BgeeiENSG00000112293.
GenevisibleiP80108. HS.

Organism-specific databases

HPAiCAB008625.
HPA012500.

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi109083. 6 interactors.
STRINGi9606.ENSP00000230036.

Structurei

3D structure databases

ProteinModelPortaliP80108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati367 – 428FG-GAP 1PROSITE-ProRule annotationAdd BLAST62
Repeati436 – 497FG-GAP 2PROSITE-ProRule annotationAdd BLAST62
Repeati499 – 559FG-GAP 3PROSITE-ProRule annotationAdd BLAST61
Repeati563 – 623FG-GAP 4PROSITE-ProRule annotationAdd BLAST61
Repeati633 – 693FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati704 – 770FG-GAP 6PROSITE-ProRule annotationAdd BLAST67
Repeati788 – 840FG-GAP 7PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the GPLD1 family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IPB3. Eukaryota.
ENOG4111ZDA. LUCA.
GeneTreeiENSGT00390000013522.
HOVERGENiHBG008185.
InParanoidiP80108.
KOiK01127.
OMAiFHGSYSE.
OrthoDBiEOG091G01DS.
PhylomeDBiP80108.
TreeFamiTF335726.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00718. PHPHLIPASED.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P80108-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAFRLWPGL LIMLGSLCHR GSPCGLSTHV EIGHRALEFL QLHNGRVNYR
60 70 80 90 100
ELLLEHQDAY QAGIVFPDCF YPSICKGGKF HDVSESTHWT PFLNASVHYI
110 120 130 140 150
RENYPLPWEK DTEKLVAFLF GITSHMAADV SWHSLGLEQG FLRTMGAIDF
160 170 180 190 200
HGSYSEAHSA GDFGGDVLSQ FEFNFNYLAR RWYVPVKDLL GIYEKLYGRK
210 220 230 240 250
VITENVIVDC SHIQFLEMYG EMLAVSKLYP TYSTKSPFLV EQFQEYFLGG
260 270 280 290 300
LDDMAFWSTN IYHLTSFMLE NGTSDCNLPE NPLFIACGGQ QNHTQGSKMQ
310 320 330 340 350
KNDFHRNLTT SLTESVDRNI NYTERGVFFS VNSWTPDSMS FIYKALERNI
360 370 380 390 400
RTMFIGGSQL SQKHVSSPLA SYFLSFPYAR LGWAMTSADL NQDGHGDLVV
410 420 430 440 450
GAPGYSRPGH IHIGRVYLIY GNDLGLPPVD LDLDKEAHRI LEGFQPSGRF
460 470 480 490 500
GSALAVLDFN VDGVPDLAVG APSVGSEQLT YKGAVYVYFG SKQGGMSSSP
510 520 530 540 550
NITISCQDIY CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG GKQKGIVAAF
560 570 580 590 600
YSGPSLSDKE KLNVEAANWT VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP
610 620 630 640 650
TWKNASRLGH LLHIRDEKKS LGRVYGYFPP NGQSWFTISG DKAMGKLGTS
660 670 680 690 700
LSSGHVLMNG TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG ATRMYALTSD
710 720 730 740 750
AQPLLLSTFS GDRRFSRFGG VLHLSDLDDD GLDEIIMAAP LRIADVTSGL
760 770 780 790 800
IGGEDGRVYV YNGKETTLGD MTGKCKSWIT PCPEEKAQYV LISPEASSRF
810 820 830 840
GSSLITVRSK AKNQVVIAAG RSSLGARLSG ALHVYSLGSD
Length:840
Mass (Da):92,336
Last modified:February 20, 2007 - v3
Checksum:i11369BAFEC3D6D38
GO
Isoform 2 (identifier: P80108-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-176: GDVLSQFEFNFN → TVYLHLLNFLVV
     177-840: Missing.

Show »
Length:176
Mass (Da):19,865
Checksum:iB71070D7DD58234B
GO

Sequence cautioni

The sequence AAA36444 differs from that shown. This sequence has numerous of conflicts with the human genome.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti531 – 534VIGS → MLGT AA sequence (PubMed:1606959).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03074317L → V.1 PublicationCorresponds to variant rs2235501dbSNPEnsembl.1
Natural variantiVAR_03074430V → I.3 PublicationsCorresponds to variant rs1126617dbSNPEnsembl.1
Natural variantiVAR_030745275D → E.Corresponds to variant rs17300770dbSNPEnsembl.1
Natural variantiVAR_051278350I → V.1 PublicationCorresponds to variant rs1062496dbSNPEnsembl.1
Natural variantiVAR_030746396G → S.Corresponds to variant rs6924628dbSNPEnsembl.1
Natural variantiVAR_030747461V → M.1 PublicationCorresponds to variant rs1062505dbSNPEnsembl.1
Natural variantiVAR_030748694M → V.1 PublicationCorresponds to variant rs1042303dbSNPEnsembl.1
Natural variantiVAR_030749698T → I.4 PublicationsCorresponds to variant rs1772256dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023261165 – 176GDVLS…EFNFN → TVYLHLLNFLVV in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_023262177 – 840Missing in isoform 2. 1 PublicationAdd BLAST664

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11701 mRNA. Translation: AAA36444.1. Sequence problems.
L11702 mRNA. Translation: AAA36445.1.
AJ308108 mRNA. Translation: CAC87068.1.
AJ400872
, AJ400873, AJ400874, AJ400875, AJ400876 Genomic DNA. Translation: CAC14844.1.
AY007546 mRNA. Translation: AAG16627.2.
AL359713, AL031230 Genomic DNA. Translation: CAI17103.1.
AL031230, AL359713 Genomic DNA. Translation: CAI22602.1.
AL031230 Genomic DNA. Translation: CAD92520.1.
BC007614 mRNA. Translation: AAH07614.1.
BC020748 mRNA. Translation: AAH20748.1.
BC093645 mRNA. Translation: AAH93645.1.
BC112001 mRNA. Translation: AAI12002.1.
CCDSiCCDS4553.1. [P80108-1]
RefSeqiNP_001494.2. NM_001503.3. [P80108-1]
UniGeneiHs.533291.

Genome annotation databases

EnsembliENST00000230036; ENSP00000230036; ENSG00000112293. [P80108-1]
GeneIDi2822.
KEGGihsa:2822.
UCSCiuc003ned.3. human. [P80108-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11701 mRNA. Translation: AAA36444.1. Sequence problems.
L11702 mRNA. Translation: AAA36445.1.
AJ308108 mRNA. Translation: CAC87068.1.
AJ400872
, AJ400873, AJ400874, AJ400875, AJ400876 Genomic DNA. Translation: CAC14844.1.
AY007546 mRNA. Translation: AAG16627.2.
AL359713, AL031230 Genomic DNA. Translation: CAI17103.1.
AL031230, AL359713 Genomic DNA. Translation: CAI22602.1.
AL031230 Genomic DNA. Translation: CAD92520.1.
BC007614 mRNA. Translation: AAH07614.1.
BC020748 mRNA. Translation: AAH20748.1.
BC093645 mRNA. Translation: AAH93645.1.
BC112001 mRNA. Translation: AAI12002.1.
CCDSiCCDS4553.1. [P80108-1]
RefSeqiNP_001494.2. NM_001503.3. [P80108-1]
UniGeneiHs.533291.

3D structure databases

ProteinModelPortaliP80108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109083. 6 interactors.
STRINGi9606.ENSP00000230036.

PTM databases

iPTMnetiP80108.
PhosphoSitePlusiP80108.

Polymorphism and mutation databases

BioMutaiGPLD1.
DMDMi126302583.

Proteomic databases

PaxDbiP80108.
PeptideAtlasiP80108.
PRIDEiP80108.

Protocols and materials databases

DNASUi2822.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230036; ENSP00000230036; ENSG00000112293. [P80108-1]
GeneIDi2822.
KEGGihsa:2822.
UCSCiuc003ned.3. human. [P80108-1]

Organism-specific databases

CTDi2822.
DisGeNETi2822.
GeneCardsiGPLD1.
H-InvDBHIX0032817.
HGNCiHGNC:4459. GPLD1.
HPAiCAB008625.
HPA012500.
MIMi602515. gene.
neXtProtiNX_P80108.
OpenTargetsiENSG00000112293.
PharmGKBiPA28842.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPB3. Eukaryota.
ENOG4111ZDA. LUCA.
GeneTreeiENSGT00390000013522.
HOVERGENiHBG008185.
InParanoidiP80108.
KOiK01127.
OMAiFHGSYSE.
OrthoDBiEOG091G01DS.
PhylomeDBiP80108.
TreeFamiTF335726.

Enzyme and pathway databases

BioCyciZFISH:HS03549-MONOMER.
BRENDAi3.1.4.50. 2681.
SignaLinkiP80108.

Miscellaneous databases

GeneWikiiGPLD1.
GenomeRNAii2822.
PROiP80108.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112293.
GenevisibleiP80108. HS.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00718. PHPHLIPASED.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHLD_HUMAN
AccessioniPrimary (citable) accession number: P80108
Secondary accession number(s): Q15127
, Q15128, Q2M2F2, Q5T3Y0, Q7Z6T8, Q8TCV0, Q8WW82, Q96ID6, Q9H167, Q9H4M1, Q9UJC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.