Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-alpha-glucanotransferase

Gene

mgtA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi13Calcium1
Metal bindingi15Calcium1
Metal bindingi17Calcium1
Metal bindingi19Calcium; via carbonyl oxygen1
Metal bindingi21Calcium1
Active sitei186Nucleophile1
Active sitei216Proton donor1
Sitei278Transition state stabilizerBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
4-alpha-glucanotransferase (EC:2.4.1.25)
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name:
D-enzyme
Gene namesi
Name:mgtA
Ordered Locus Names:TM_0364
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543361 – 4414-alpha-glucanotransferaseAdd BLAST441

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi243274.TM0364.

Structurei

Secondary structure

1441
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Helixi8 – 11Combined sources4
Beta strandi14 – 19Combined sources6
Helixi22 – 27Combined sources6
Helixi29 – 34Combined sources6
Beta strandi39 – 42Combined sources4
Beta strandi49 – 52Combined sources4
Beta strandi57 – 62Combined sources6
Turni64 – 66Combined sources3
Helixi69 – 81Combined sources13
Beta strandi85 – 90Combined sources6
Helixi100 – 106Combined sources7
Helixi110 – 113Combined sources4
Beta strandi130 – 132Combined sources3
Beta strandi137 – 139Combined sources3
Beta strandi145 – 147Combined sources3
Beta strandi159 – 161Combined sources3
Helixi162 – 176Combined sources15
Turni177 – 179Combined sources3
Beta strandi182 – 185Combined sources4
Helixi188 – 190Combined sources3
Beta strandi191 – 194Combined sources4
Helixi195 – 205Combined sources11
Turni206 – 208Combined sources3
Beta strandi211 – 215Combined sources5
Helixi221 – 231Combined sources11
Beta strandi232 – 235Combined sources4
Helixi237 – 248Combined sources12
Helixi253 – 262Combined sources10
Beta strandi267 – 274Combined sources8
Helixi282 – 284Combined sources3
Turni285 – 287Combined sources3
Helixi291 – 302Combined sources12
Beta strandi304 – 311Combined sources8
Turni312 – 317Combined sources6
Helixi327 – 330Combined sources4
Beta strandi336 – 340Combined sources5
Beta strandi356 – 359Combined sources4
Helixi362 – 365Combined sources4
Helixi372 – 385Combined sources14
Helixi387 – 389Combined sources3
Beta strandi393 – 399Combined sources7
Beta strandi401 – 410Combined sources10
Beta strandi413 – 420Combined sources8
Beta strandi422 – 424Combined sources3
Beta strandi426 – 428Combined sources3
Beta strandi431 – 433Combined sources3
Beta strandi438 – 440Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LWHX-ray2.60A/B1-441[»]
1LWJX-ray2.50A/B1-441[»]
ProteinModelPortaliP80099.
SMRiP80099.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80099.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105CG3. Bacteria.
COG0366. LUCA.
InParanoidiP80099.
KOiK00705.
OMAiWAEARMV.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015261. 4-alpha-glucanotransf_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09178. DUF1945. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P80099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGYQIYVRS FRDGNLDGVG DFRGLKNAVS YLKELGIDFV WLMPVFSSIS
60 70 80 90 100
FHGYDVVDFY SFKAEYGSER EFKEMIEAFH DSGIKVVLDL PIHHTGFLHT
110 120 130 140 150
WFQKALKGDP HYRDYYVWAN KETDLDERRE WDGEKIWHPL EDGRFYRGLF
160 170 180 190 200
GPFSPDLNYD NPQVFDEMKR LVLHLLDMGV DGFRFDAAKH MRDTIEQNVR
210 220 230 240 250
FWKYFLSDLK GIFLAEIWAE ARMVDEHGRI FGYMLNFDTS HCIKEAVWKE
260 270 280 290 300
NTRVLIESIE RAVIGKDYLP VNFTSNHDMS RLASFEGGFS KEKIKLSISI
310 320 330 340 350
LFTLPGVPLV FYGDELGMKG VYQKPNTEVV LDPFPWNESM CVEGQTFWKW
360 370 380 390 400
PAYNGPFSGI SVEYQKRDPD SILSHTLGWT RFRKENQWID RAKLEFLCKE
410 420 430 440
DKFLVYRLYD DQHSLKVFHN LSGEEVVFEG VKMKPYKTEV V
Length:441
Mass (Da):51,843
Last modified:May 30, 2000 - v2
Checksum:i9FB4C2ABC9D3DF3A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → A AA sequence (PubMed:1628664).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35451.1.
RefSeqiNP_228175.1. NC_000853.1.
WP_004083169.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35451; AAD35451; TM_0364.
GeneIDi897323.
KEGGitma:TM0364.
PATRICi23935609. VBITheMar51294_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35451.1.
RefSeqiNP_228175.1. NC_000853.1.
WP_004083169.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LWHX-ray2.60A/B1-441[»]
1LWJX-ray2.50A/B1-441[»]
ProteinModelPortaliP80099.
SMRiP80099.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0364.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

DNASUi897323.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35451; AAD35451; TM_0364.
GeneIDi897323.
KEGGitma:TM0364.
PATRICi23935609. VBITheMar51294_0369.

Phylogenomic databases

eggNOGiENOG4105CG3. Bacteria.
COG0366. LUCA.
InParanoidiP80099.
KOiK00705.
OMAiWAEARMV.

Miscellaneous databases

EvolutionaryTraceiP80099.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015261. 4-alpha-glucanotransf_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09178. DUF1945. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMGTA_THEMA
AccessioniPrimary (citable) accession number: P80099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.