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P80099

- MGTA_THEMA

UniProt

P80099 - MGTA_THEMA

Protein

4-alpha-glucanotransferase

Gene

mgtA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

    Cofactori

    Binds 1 calcium ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi13 – 131Calcium
    Metal bindingi15 – 151Calcium
    Metal bindingi17 – 171Calcium
    Metal bindingi19 – 191Calcium; via carbonyl oxygen
    Metal bindingi21 – 211Calcium
    Active sitei186 – 1861Nucleophile
    Active sitei216 – 2161Proton donor
    Sitei278 – 2781Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. 4-alpha-glucanotransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-alpha-glucanotransferase (EC:2.4.1.25)
    Alternative name(s):
    Amylomaltase
    Disproportionating enzyme
    Short name:
    D-enzyme
    Gene namesi
    Name:mgtA
    Ordered Locus Names:TM_0364
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4414414-alpha-glucanotransferasePRO_0000054336Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi243274.TM0364.

    Structurei

    Secondary structure

    1
    441
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Helixi8 – 114
    Beta strandi14 – 196
    Helixi22 – 276
    Helixi29 – 346
    Beta strandi39 – 424
    Beta strandi49 – 524
    Beta strandi57 – 626
    Turni64 – 663
    Helixi69 – 8113
    Beta strandi85 – 906
    Helixi100 – 1067
    Helixi110 – 1134
    Beta strandi130 – 1323
    Beta strandi137 – 1393
    Beta strandi145 – 1473
    Beta strandi159 – 1613
    Helixi162 – 17615
    Turni177 – 1793
    Beta strandi182 – 1854
    Helixi188 – 1903
    Beta strandi191 – 1944
    Helixi195 – 20511
    Turni206 – 2083
    Beta strandi211 – 2155
    Helixi221 – 23111
    Beta strandi232 – 2354
    Helixi237 – 24812
    Helixi253 – 26210
    Beta strandi267 – 2748
    Helixi282 – 2843
    Turni285 – 2873
    Helixi291 – 30212
    Beta strandi304 – 3118
    Turni312 – 3176
    Helixi327 – 3304
    Beta strandi336 – 3405
    Beta strandi356 – 3594
    Helixi362 – 3654
    Helixi372 – 38514
    Helixi387 – 3893
    Beta strandi393 – 3997
    Beta strandi401 – 41010
    Beta strandi413 – 4208
    Beta strandi422 – 4243
    Beta strandi426 – 4283
    Beta strandi431 – 4333
    Beta strandi438 – 4403

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LWHX-ray2.60A/B1-441[»]
    1LWJX-ray2.50A/B1-441[»]
    ProteinModelPortaliP80099.
    SMRiP80099. Positions 1-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP80099.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    KOiK00705.
    OMAiLDERREW.
    OrthoDBiEOG6RZB0T.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015261. 4-alpha-glucanotransf_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF09178. DUF1945. 1 hit.
    [Graphical view]
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P80099-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIGYQIYVRS FRDGNLDGVG DFRGLKNAVS YLKELGIDFV WLMPVFSSIS    50
    FHGYDVVDFY SFKAEYGSER EFKEMIEAFH DSGIKVVLDL PIHHTGFLHT 100
    WFQKALKGDP HYRDYYVWAN KETDLDERRE WDGEKIWHPL EDGRFYRGLF 150
    GPFSPDLNYD NPQVFDEMKR LVLHLLDMGV DGFRFDAAKH MRDTIEQNVR 200
    FWKYFLSDLK GIFLAEIWAE ARMVDEHGRI FGYMLNFDTS HCIKEAVWKE 250
    NTRVLIESIE RAVIGKDYLP VNFTSNHDMS RLASFEGGFS KEKIKLSISI 300
    LFTLPGVPLV FYGDELGMKG VYQKPNTEVV LDPFPWNESM CVEGQTFWKW 350
    PAYNGPFSGI SVEYQKRDPD SILSHTLGWT RFRKENQWID RAKLEFLCKE 400
    DKFLVYRLYD DQHSLKVFHN LSGEEVVFEG VKMKPYKTEV V 441
    Length:441
    Mass (Da):51,843
    Last modified:May 30, 2000 - v2
    Checksum:i9FB4C2ABC9D3DF3A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 11M → A AA sequence (PubMed:1628664)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35451.1.
    RefSeqiNP_228175.1. NC_000853.1.
    YP_007976712.1. NC_021214.1.
    YP_008990663.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35451; AAD35451; TM_0364.
    GeneIDi897323.
    KEGGitma:TM0364.
    tmi:THEMA_02895.
    tmm:Tmari_0362.
    PATRICi23935609. VBITheMar51294_0369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35451.1 .
    RefSeqi NP_228175.1. NC_000853.1.
    YP_007976712.1. NC_021214.1.
    YP_008990663.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LWH X-ray 2.60 A/B 1-441 [» ]
    1LWJ X-ray 2.50 A/B 1-441 [» ]
    ProteinModelPortali P80099.
    SMRi P80099. Positions 1-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0364.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    DNASUi 897323.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35451 ; AAD35451 ; TM_0364 .
    GeneIDi 897323.
    KEGGi tma:TM0364.
    tmi:THEMA_02895.
    tmm:Tmari_0362.
    PATRICi 23935609. VBITheMar51294_0369.

    Phylogenomic databases

    eggNOGi COG0366.
    KOi K00705.
    OMAi LDERREW.
    OrthoDBi EOG6RZB0T.

    Miscellaneous databases

    EvolutionaryTracei P80099.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015261. 4-alpha-glucanotransf_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF09178. DUF1945. 1 hit.
    [Graphical view ]
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Purification and characterization of a novel thermostable 4-alpha-glucanotransferase of Thermotoga maritima cloned in Escherichia coli."
      Liebl W., Feil R., Gabelsberger J., Kellermann J., Schleifer K.-H.
      Eur. J. Biochem. 207:81-88(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-23, CHARACTERIZATION.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "Crystal structure of Thermotoga maritima 4-alpha-glucanotransferase and its acarbose complex: implications for substrate specificity and catalysis."
      Roujeinikova A., Raasch C., Sedelnikova S., Liebl W., Rice D.W.
      J. Mol. Biol. 321:149-162(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

    Entry informationi

    Entry nameiMGTA_THEMA
    AccessioniPrimary (citable) accession number: P80099
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3