P80098 (CCL7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C motif chemokine 7

Alternative name(s):
Monocyte chemoattractant protein 3
Monocyte chemotactic protein 3
Short name=MCP-3
NC28
Small-inducible cytokine A7

Gene names

Name:	CCL7
Synonyms:	MCP3, SCYA6, SCYA7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	99 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Subunit structure	Monomer. Ref.8
Subcellular location	Secreted.
Post-translational modification	O-glycosylated.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.
Sequence caution	The sequence CAA50405.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA50406.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA51055.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Domain	Signal
Ligand	Heparin-binding
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.2. Source: ProtInc cellular calcium ion homeostasis Traceable author statement PubMed 10770925. Source: ProtInc cellular response to ethanol Inferred from electronic annotation. Source: Compara cytoskeleton organization Inferred from direct assay PubMed 10072545. Source: UniProtKB eosinophil chemotaxis Inferred from direct assay PubMed 10072545. Source: UniProtKB immune response Inferred from electronic annotation. Source: InterPro inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW monocyte chemotaxis Inferred by curator PubMed 10660125. Source: UniProtKB positive regulation of natural killer cell chemotaxis Inferred from direct assay PubMed 7545673. Source: UniProtKB regulation of cell shape Inferred from direct assay PubMed 10072545. Source: UniProtKB response to gamma radiation Inferred from electronic annotation. Source: Compara signal transduction Traceable author statement PubMed 10201901PubMed 10770925. Source: ProtInc
Cellular_component	extracellular space Traceable author statement Ref.2. Source: ProtInc
Molecular_function	chemokine activity Inferred from direct assay PubMed 10072545. Source: UniProtKB heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.7

Chain

24 – 99

76

C-C motif chemokine 7

PRO_0000005183

Amino acid modifications

Modified residue

24

1

Pyrrolidone carboxylic acid

Glycosylation

29

1

N-linked (GlcNAc...) Potential

Disulfide bond

34 ↔ 59

Disulfide bond

35 ↔ 75

Experimental info

Sequence conflict

30

1

T → K AA sequence Ref.6

Sequence conflict

68 – 70

3

Missing AA sequence Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

99

Helix Strand Turn

Details...

Beta strand

29 – 31

3

Beta strand

33 – 35

3

Turn

45 – 47

3

Beta strand

48 – 53

6

Turn

56 – 58

3

Beta strand

64 – 68

5

Beta strand

73 – 76

4

Helix

81 – 91

11

Sequences

	Sequence		Length	Mass (Da)
	P80098 [UniParc]. Last modified November 1, 1995. Version 3. Checksum: 96048B371C25D00E	FASTA	99	11,200
	10 20 30 40 50 60 MKASAALLCL LLTAAAFSPQ GLAQPVGINT STTCCYRFIN KKIPKQRLES YRRTTSSHCP 70 80 90 REAVIFKTKL DKEICADPTQ KWVQDFMKHL DKKTQTPKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines." Opdenakker G., Froyen G., Fiten P., Proost P., van Damme J. Biochem. Biophys. Res. Commun. 191:535-542(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 31-67 AND 71-99.
[2]	"The human MCP-3 gene (SCYA7): cloning, sequence analysis, and assignment to the C-C chemokine gene cluster on chromosome 17q11.2-q12." Opdenakker G., Fiten P., Nys G., Froyen G., van Roy N., Speleman F., Laureys G., van Damme J. Genomics 21:403-408(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Molecular cloning of the MCP-3 chemokine gene and regulation of its expression." Minty A., Chalon P., Guillemot J.-C., Kaghad M., Liauzun P., Magazin M., Miloux B., Minty C., Ramond P., Vita N., Lupker J., Shire D., Ferrara P., Caput D. Eur. Cytokine Netw. 4:99-110(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood and Colon.
[5]	Jang J.S., Kim B.E. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-99.
[6]	"Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family." van Damme J., Proost P., Lenaerts J.-P., Opdenakker G. J. Exp. Med. 176:59-65(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-99. Tissue: Osteosarcoma.
[7]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-38.
[8]	"Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3." Kim K.-S., Rajarathnam K., Clark-Lewis I., Sykes B.D. FEBS Lett. 395:277-282(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, SUBUNIT.
[9]	"Determination of the three-dimensional structure of CC chemokine monocyte chemoattractant protein 3 by 1H two-dimensional NMR spectroscopy." Meunier S., Bernassau J.-M., Guillemot J.-C., Ferrara P., Darbon H. Biochemistry 36:4412-4422(1997) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[10]	Kwon D., Lee D., Sykes B.D., Kim K.-S. Submitted (AUG-1998) to the PDB data bank Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Web resources

Wikipedia

CCL7 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X72308 mRNA. Translation: CAA51055.1. Different initiation.
X72309 Genomic DNA. No translation available.
X71087 mRNA. Translation: CAA50407.1.
X71087 mRNA. Translation: CAA50406.1. Different initiation.
X71087 mRNA. Translation: CAA50405.1. Different initiation.
BC092436 mRNA. Translation: AAH92436.1.
BC112258 mRNA. Translation: AAI12259.1.
BC112260 mRNA. Translation: AAI12261.1.
AF043338 mRNA. Translation: AAC03538.1.

IPI

IPI00746376.

RefSeq

NP_006264.2. NM_006273.3.

UniGene

Hs.251526.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BO0	NMR	-	A	24-99	[»]
1NCV	NMR	-	A/B	24-99	[»]

ProteinModelPortal

P80098.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5847N.

IntAct

P80098. 2 interactions.

MINT

MINT-103194.

STRING

9606.ENSP00000200307.

Polymorphism databases

DMDM

1170890.

Proteomic databases

PaxDb

P80098.

PRIDE

P80098.

Protocols and materials databases

DNASU

6354.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000378569; ENSP00000367832; ENSG00000108688.

GeneID

6354.

KEGG

hsa:6354.

UCSC

uc002hhz.3. human.

Organism-specific databases

CTD

6354.

GeneCards

GC17P032597.

HGNC

HGNC:10634. CCL7.

MIM

158106. gene.

neXtProt

NX_P80098.

PharmGKB

PA35566.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG140372.

HOGENOM

HOG000036686.

HOVERGEN

HBG017871.

InParanoid

P80098.

KO

K05509.

OrthoDB

EOG45DWR1.

PhylomeDB

P80098.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P80098.

Bgee

P80098.

CleanEx

HS_CCL7.

Genevestigator

P80098.

GermOnline

ENSG00000108688. Homo sapiens.

Family and domain databases

InterPro

IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P80098.

GenomeRNAi

6354.

NextBio

24680.

PMAP-CutDB

P80098.

SOURCE

Search...

Entry information

Entry name

CCL7_HUMAN

Accession

Primary (citable) accession number: P80098
Secondary accession number(s): Q569J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 127 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.