P80092 (CBPX1_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thermostable carboxypeptidase 1
EC=3.4.17.-

Gene names

Name:	cpsA1
Synonyms:	cpsA, cpsA-1
Ordered Locus Names:	SSO1355

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids.
Cofactor	Zinc.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the peptidase M20 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxypeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Thermostable carboxypeptidase 1		PRO_0000061947
Sites
	Active site	302	1	Proton donor Potential
	Active site	373	1	Nucleophile Potential
	Metal binding	104	1	Zinc Potential
	Metal binding	109	1	Zinc Potential
	Metal binding	245	1	Zinc Potential
Experimental info
	Sequence conflict	12	1	R → K AA sequence Ref.3
	Sequence conflict	17	1	W → R AA sequence Ref.3
	Sequence conflict	28	1	Y → N AA sequence Ref.3
	Sequence conflict	30	1	E → P AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P80092 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: B4754EC5A8E9A9C8	FASTA	393	43,069
	10 20 30 40 50 60 MDLVEKLKND VREIEDWIIQ IRRKIHEYPE LSYKEYNTSK LVAETLRKLG VEVEEGVGLP 70 80 90 100 110 120 TAVVGKIRGS KPGKTVALRA DMDALPVEEN TDLEFKSKVK GVMHACGHDT HVAMLLGGAY 130 140 150 160 170 180 LLVKNKDLIS GEIRLIFQPA EEDGGLGGAK PMIEAGVMNG VDYVFGIHIS SSYPSGVFAT 190 200 210 220 230 240 RKGPIMATPD AFKIIVHGKG GHGSAPHETI DPIFISLQIA NAIYGITARQ IDPVQPFIIS 250 260 270 280 290 300 ITTIHSGTKD NIIPDDAEMQ GTIRSLDENV RSKAKDYMRR IVSSICGIYG ATCEVKFMED 310 320 330 340 350 360 VYPTTVNNPE VTDEVMKILS SISTVVETEP VLGAEDFSRF LQKAPGTYFF LGTRNEKKGC 370 380 390 IYPNHSSKFC VDEDVLKLGA LAHALLAVKF SNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, nucleotide sequence, and expression of a carboxypeptidase-encoding gene from the archaebacterium Sulfolobus solfataricus." Colombo S., Toietta G., Zecca L., Vanoni M., Tortora P. J. Bacteriol. 177:5561-5566(1995) [PubMed: 7559343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 5833 / MT-4.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus." Colombo S., D'Auria S., Fusi P., Zecca L., Raia C.A., Tortora P. Eur. J. Biochem. 206:349-357(1992) [PubMed: 1597179] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-31. Strain: DSM 5833 / MT-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48497 Genomic DNA. Translation: CAA88397.1. AE006641 Genomic DNA. Translation: AAK41591.1.
PIR	H90291. S23180.
RefSeq	NP_342801.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P80092.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454370.
GenomeReviews	Gene locus SSO1355 in contig AE006641_GR.
KEGG	sso:SSO1355.
NMPDR	fig|273057.1.peg.1224.
Phylogenomic databases
HOGENOM	HBG708500.
OMA	IVEINHS.
ProtClustDB	CLSK785363.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO1355-MONOMER.
Family and domain databases
InterPro	IPR017439. Amidohydrolase. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
KO	K13048.
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAM	SSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMs	TIGR01891. Amidohydrolases. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CBPX1_SULSO

Accession

Primary (citable) accession number: P80092

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program