Reviewed, UniProtKB/Swiss-Prot P80057 (GSEP_BACLD)

Names and origin

Protein names

Recommended name:
    Glutamyl endopeptidase
    EC=3.4.21.19
Alternative name(s):
    Glutamate-specific endopeptidase
      Short name=GSE

Gene names

Name:	blaSE
Synonyms:	mpr
Ordered Locus Names:	BLi00340, BL01804

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specific for hydrolysis of peptide bonds on the carboxyl side of acidic amino acid residues, with a strong preference for Glu.
Catalytic activity	Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1B family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Propeptide	31 – 94	64	Ref.4		PRO_0000026900
	Chain	95 – 316	222	Glutamyl endopeptidase		PRO_0000026901
Sites
	Active site	141	1	Charge relay system By similarity
	Active site	261	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	126 ↔ 142
	Disulfide bond	275 ↔ 279

Sequences

	Sequence		Length	Mass (Da)
	P80057-1 [UniParc]. Last modified October 1, 1993. Version 2. Checksum: 96D7552CB7089B09	FASTA	316	33,611
	10 20 30 40 50 60 MVSKKSVKRG LITGLIGISI YSLGMHPAQA APSPHTPVSS DPSYKAETSV TYDPNIKSDQ 70 80 90 100 110 120 YGLYSKAFTG TGKVNETKEK AEKKSPAKAP YSIKSVIGSD DRTRVTNTTA YPYRAIVHIS 130 140 150 160 170 180 SSIGSCTGWM IGPKTVATAG HCIYDTSSGS FAGTATVSPG RNGTSYPYGS VKSTRYFIPS 190 200 210 220 230 240 GWRSGNTNYD YGAIELSEPI GNTVGYFGYS YTTSSLVGTT VTISGYPGDK TAGTQWQHSG 250 260 270 280 290 300 PIAISETYKL QYAMDTYGGQ SGSPVFEQSS SRTNCSGPCS LAVHTNGVYG GSSYNRGTRI 310 TKEVFDNLTN WKNSAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, characterization, cloning, and expression of a glutamic acid-specific protease from Bacillus licheniformis ATCC 14580." Kakudo S., Kikuchi N., Kitadokoro K., Fujiwara T., Nakamura E., Okamoto H., Shin M., Tamaki M., Teraoka H., Tsuzuki H., Yoshida N. J. Biol. Chem. 267:23782-23788(1992) [PubMed: 1429718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Isolation and amino acid sequence of a glutamic acid specific endopeptidase from Bacillus licheniformis." Svendsen I., Breddam K. Eur. J. Biochem. 204:165-171(1992) [PubMed: 1346764] [Abstract] Cited for: PROTEIN SEQUENCE OF 95-316.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10060 Genomic DNA. Translation: BAA00949.1. AE017333 Genomic DNA. Translation: AAU39298.1. CP000002 Genomic DNA. Translation: AAU21945.1.
PIR	A45134.
RefSeq	YP_077583.1. YP_089991.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S01.271.
Genome annotation databases
GeneID	3028461. 3101563.
GenomeReviews	Gene locus BLi00340 in contig AE017333_GR. Gene locus BL01804 in contig CP000002_GR.
KEGG	bld:BLi00340. bli:BL01804.
NMPDR	fig|279010.5.peg.2193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P80057.
OMA	P80057. PYRAIVH.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01804-MON.
Family and domain databases
InterPro	IPR000126. Pept_S1B_AS. IPR001254. Peptidase_S1_S6. IPR008256. Peptidase_S1B. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00839. V8PROTEASE.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00672. V8_HIS. 1 hit. PS00673. V8_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSEP_BACLD

Accession

Primary (citable) accession number: P80057
Secondary accession number(s): Q65NR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)