Reviewed, UniProtKB/Swiss-Prot P80053 (DHE2_SULSO)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase 2
      Short name=GDH-2
    EC=1.4.1.3

Gene names

Name:	gdhA-2
Synonyms:	gdhA
Ordered Locus Names:	SSO1907

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Subunit structure	Homohexamer.
Post-translational modification	Methylation of lysine residues may play a role in the thermal stability of this enzyme.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD NADP
Molecular function	Oxidoreductase
PTM	Acetylation Methylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 420	419	Glutamate dehydrogenase 2		PRO_0000182761
Sites
	Active site	109	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylmethionine
	Modified residue	254	1	N6-methyllysine
	Modified residue	260	1	N6-methyllysine
	Modified residue	372	1	N6-methyllysine
	Modified residue	391	1	N6-methyllysine
	Modified residue	392	1	N6-methyllysine
Experimental info
	Sequence conflict	203	1	E → EE AA sequence Ref.2
	Sequence conflict	392	1	K → KK AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80053-1 [UniParc]. Last modified March 18, 2008. Version 3. Checksum: D9283C66D305EED5	FASTA	420	46,031
	10 20 30 40 50 60 MMEEVLSSSL YTQQVKKLYK VGELLGLDNE TLETLSQPER IIQVKIQIRG SDGKLKTFMG 70 80 90 100 110 120 WRSQHNSALG PYKGGVRYHP NVTQDEVEAL SMIMTWKNSL LLLPYGGGKG GVRVDPKKLT 130 140 150 160 170 180 REELEQLSRK YIQAIYKYLG SELDIPAPDV NTDSQTMAWF LDEYIKITGK VDFAVFTGKP 190 200 210 220 230 240 VELGGIGVRL YSTGLGVATI AKEAANKFIG GVEEARVIIQ GFGNVGYYAG KFLSEMGAKI 250 260 270 280 290 300 VGVSDSKGGV INEKGIDVGK AIEIKEKTGS VINYPEGRKV TNEELLISDC DILIPAALEN 310 320 330 340 350 360 VINKFNAPKV KAKLIVEGAN GPLTADADEI MRQRGIAVVP DILANAGGVV GSYVEWANNK 370 380 390 400 410 420 MGEIISDEEA KKLIVDRMNN AFNTLYDYHQ KKLEDHDLRT AAMALAVDRV VRAMKARGIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The protein sequence of glutamate dehydrogenase from Sulfolobus solfataricus, a thermoacidophilic archaebacterium. Is the presence of N-epsilon-methyllysine related to thermostability?" Maras B., Consalvi V., Chiaraluce R., Politi L., de Rosa M., Bossa F., Scandurra R., Barra D. Eur. J. Biochem. 203:81-87(1992) [PubMed: 1730244] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-420. Strain: DSM 5833 / MT-4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006641 Genomic DNA. Translation: AAK42099.1.
PIR	D90355. S20286.
RefSeq	NP_343309.1.
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Genome annotation databases
GeneID	1453426.
GenomeReviews	Gene locus SSO1907 in contig AE006641_GR.
KEGG	sso:SSO1907.
NMPDR	fig|273057.1.peg.1732.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P80053.
OMA	P80053. ANNKMGE.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO1907-MON.
BRENDA	1.4.1.3. 2070.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE2_SULSO

Accession

Primary (citable) accession number: P80053

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 18, 2008
Last modified:	June 16, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)