Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Enzyme regulationi

Inhibition of this enzyme by phosphorylation regulates the branch point between the Krebs cycle and the glyoxylate bypass, which is an alternate route that accumulates carbon for biosynthesis when acetate is the sole carbon source for growth.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104NADPBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei115SubstrateBy similarity1
Binding sitei119SubstrateBy similarity1
Binding sitei129SubstrateBy similarity1
Binding sitei153SubstrateBy similarity1
Sitei160Critical for catalysisBy similarity1
Sitei237Critical for catalysisBy similarity1
Metal bindingi362Magnesium or manganeseBy similarity1
Binding sitei407NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei446NADPBy similarity1
Binding sitei450NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi394 – 400NADPBy similarity7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:icd
Ordered Locus Names:slr1289
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835701 – 475Isocitrate dehydrogenase [NADP]Add BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP80046.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP80046. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP80046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000021113.
InParanoidiP80046.
KOiK00031.
PhylomeDBiP80046.

Family and domain databases

Gene3Di3.40.718.10. 2 hits.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_dimer_prok.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 3 hits.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00183. prok_nadp_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEKLQPPSV GSKITFVAGK PVVPNDPIIP YIRGDGTGVD IWPATELVIN
60 70 80 90 100
AAIAKAYGGR EEINWFKVYA GDEACELYGT YQIFPEDTLT AIKEYGVAIK
110 120 130 140 150
GPLTTPVGGG IRSLNVALRQ IFDLYTCVRP CRYYPGTPSP HKTPEKLDII
160 170 180 190 200
VYRENTEDIY LGIEWAEGTE GAKKLIAYLN DELIPTTPAL GKKQIRLDSG
210 220 230 240 250
IGIKPISKTG SQRLVRRAIL HAKRLPKAKQ MVTLVHKGNI MKFTEGPFRD
260 270 280 290 300
WGYELATTEF RAECVTERES WICGNKESNP DLTIEANAHM IDPGYDTLTE
310 320 330 340 350
EKQAVIKQEV EQVLNSIWES HGNGQWKEKV MVNDRIADSI FQQIQTRPDE
360 370 380 390 400
YSILATMNLN GDYLSDAAAA VVGGLGMGPG ANIGDSAAIF EATHGTAPKH
410 420 430 440 450
AGLDRINPGS VILSGVMMLE FMGWQEAADL IKKGIGAAIA NREVTYDLAR
460 470
LMEPKVDKPL KCSEFAQAIV SHFDD
Length:475
Mass (Da):52,275
Last modified:November 1, 1997 - v2
Checksum:i6F3254A8BBA75F92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60 – 62REE → ERK in BAA16835 (PubMed:8905231).Curated3
Sequence conflicti83 – 84IF → YL in BAA16835 (PubMed:8905231).Curated2
Sequence conflicti223K → L in BAA16835 (PubMed:8905231).Curated1
Sequence conflicti247P → A in BAA16835 (PubMed:8905231).Curated1
Sequence conflicti273C → L in BAA16835 (PubMed:8905231).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83563 Genomic DNA. Translation: CAA58549.1.
BA000022 Genomic DNA. Translation: BAA16835.1.
PIRiS74684.

Genome annotation databases

EnsemblBacteriaiBAA16835; BAA16835; BAA16835.
KEGGisyn:slr1289.
PATRICi23837428. VBISynSp132158_0286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83563 Genomic DNA. Translation: CAA58549.1.
BA000022 Genomic DNA. Translation: BAA16835.1.
PIRiS74684.

3D structure databases

ProteinModelPortaliP80046.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP80046. 2 interactors.

Proteomic databases

PRIDEiP80046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA16835; BAA16835; BAA16835.
KEGGisyn:slr1289.
PATRICi23837428. VBISynSp132158_0286.

Phylogenomic databases

HOGENOMiHOG000021113.
InParanoidiP80046.
KOiK00031.
PhylomeDBiP80046.

Family and domain databases

Gene3Di3.40.718.10. 2 hits.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_dimer_prok.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 3 hits.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00183. prok_nadp_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDH_SYNY3
AccessioniPrimary (citable) accession number: P80046
Secondary accession number(s): P72820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.