Reviewed, UniProtKB/Swiss-Prot P80042 (GGPPS_CAPAN)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl pyrophosphate synthetase, chloroplastic
      Short name=GGPP synthetase
      Short name=GGPS
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29

Gene names

Name:

GGPS1

Organism

Capsicum annuum (Bell pepper)

Taxonomic identifier

4072 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Pathway	Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranylgeranyl-PP biosynthesis; geranylgeranyl-PP from farnesyl-PP and isopentenyl-PP: step 1/1.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dimethylallyltranstransferase activity Ref.1 Inferred from direct assay. Source: UniProtKB farnesyltranstransferase activity Ref.1 Inferred from direct assay. Source: UniProtKB geranyltranstransferase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast
	Chain	? – 369		Geranylgeranyl pyrophosphate synthetase, chloroplastic		PRO_0000016472

Sequences

	Sequence		Length	Mass (Da)
	P80042-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 2D527F5E43A29C6F	FASTA	369	40,173
	10 20 30 40 50 60 MRSMNLVDLW AQQACLVFNQ TLSYKSFNGF MKIPLKNSKI NPKLNKKRPF SPLTVSAIAT 70 80 90 100 110 120 TKEDERIEAA QTEEPFNFKI YVTEKAISVN KALDEAIIVK EPHVIHEAMR YSLLAGGKRV 130 140 150 160 170 180 RPMLCLAACE LVGGNQENAM AAACAVEMIH TMSLIHDDLP CMDNDDLRRG KPTNHKIYGE 190 200 210 220 230 240 DVAVLAGDSL LAFAFEHIVN STAGVTPSRI VGAVAELAKS IGTEGLVAGQ VADIKCTGNA 250 260 270 280 290 300 SVSLETLEFI HVHKTAALLE SSVVLGAILG GGTNVEVEKL RRFARCIGLL FQVVDDILDV 310 320 330 340 350 360 TKSSEELGKT AGKDLVVDKT TYPKLLGLEK AKEFAAELNR EAKQQLEGFD SRKAAPLIAL ADYIAYRDN

References

[1]

"Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening." Kuntz M., Roemer S., Suire C., Hugueney P., Weil J.H., Schantz R., Camara B. Plant J. 2:25-34(1992) [PubMed: 1303794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Lamuyo. Tissue: Fruit.

[2]

"Structure of a functional geranylgeranyl pyrophosphate synthase gene from Capsicum annuum." Badillo A., Steppuhn J., Deruere J., Camara B., Kuntz M. Plant Mol. Biol. 27:425-428(1995) [PubMed: 7888631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Yolo Wonder.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80267 Genomic DNA. Translation: CAA56554.1.
PIR	S53722.
3D structure databases
SMR	P80042. Positions 81-369.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12171.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGPPS_CAPAN

Accession

Primary (citable) accession number: P80042

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)