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Protein

Malate dehydrogenase

Gene

mdh

Organism
Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADUniRule annotation2 Publications1
Binding sitei82SubstrateUniRule annotationBy similarity1
Binding sitei88SubstrateUniRule annotationBy similarity1
Binding sitei95NADUniRule annotationBy similarity1
Binding sitei120SubstrateUniRule annotationBy similarity1
Binding sitei151SubstrateUniRule annotationBy similarity1
Active sitei175Proton acceptorUniRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14NADUniRule annotation2 Publications6
Nucleotide bindingi118 – 120NADUniRule annotation2 Publications3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciCAUR324602:G1GAO-911-MONOMER
BRENDAi1.1.1.37 1352

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Caur_0900
OrganismiChloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
Taxonomic identifieri324602 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesChloroflexineaeChloroflexaceaeChloroflexus
Proteomesi
  • UP000002008 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187T → C: Forms an intersubunit disulfide bridge, which makes the enzyme more resistant to thermal denaturation. The mutation does not alter the quaternary structure of the enzyme. 1

Chemistry databases

DrugBankiDB01677 Fumarate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134471 – 309Malate dehydrogenaseAdd BLAST309

Proteomic databases

PRIDEiP80040

Interactioni

Subunit structurei

Homotetramer (active enzyme); homodimer and homotrimer at temperatures lower than 55 degrees Celsius (inactive forms).3 Publications

Protein-protein interaction databases

STRINGi324602.Caur_0900

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi12 – 23Combined sources12
Beta strandi27 – 32Combined sources6
Beta strandi34 – 37Combined sources4
Helixi38 – 47Combined sources10
Helixi50 – 53Combined sources4
Beta strandi59 – 63Combined sources5
Helixi65 – 68Combined sources4
Beta strandi72 – 76Combined sources5
Helixi90 – 106Combined sources17
Helixi107 – 109Combined sources3
Beta strandi114 – 117Combined sources4
Beta strandi119 – 121Combined sources3
Helixi122 – 133Combined sources12
Helixi137 – 139Combined sources3
Beta strandi140 – 142Combined sources3
Helixi145 – 160Combined sources16
Helixi164 – 166Combined sources3
Beta strandi167 – 169Combined sources3
Beta strandi172 – 175Combined sources4
Helixi176 – 178Combined sources3
Helixi183 – 185Combined sources3
Beta strandi186 – 188Combined sources3
Helixi193 – 195Combined sources3
Helixi199 – 210Combined sources12
Helixi212 – 220Combined sources9
Beta strandi221 – 223Combined sources3
Helixi227 – 241Combined sources15
Beta strandi246 – 256Combined sources11
Helixi257 – 259Combined sources3
Beta strandi261 – 272Combined sources12
Beta strandi275 – 279Combined sources5
Helixi286 – 306Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUYX-ray2.20A/C1-309[»]
1UR5X-ray1.75A/C1-309[»]
1UXGX-ray1.90A/B1-309[»]
1UXHX-ray2.10A/B1-309[»]
1UXIX-ray2.10A/B1-309[»]
1UXJX-ray1.75A/C1-309[»]
1UXKX-ray1.80A/C1-309[»]
4CL3X-ray1.70A/D1-309[»]
ProteinModelPortaliP80040
SMRiP80040
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80040

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213794
InParanoidiP80040
KOiK00024
OrthoDBiPOG091H02N6

Family and domain databases

CDDicd01339 LDH-like_MDH, 1 hit
Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00487 Malate_dehydrog_3, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR011275 Malate_DH_type3
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01763 MalateDH_bact, 1 hit

Sequencei

Sequence statusi: Complete.

P80040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKISIIGA GFVGSTTAHW LAAKELGDIV LLDFVEGVPQ GKALDLYEAS
60 70 80 90 100
PIEGFDVRVT GTNNYADTAN SDVIVVTSGA PRKPGMSRED LIKVNADITR
110 120 130 140 150
ACISQAAPLS PNAVIIMVNN PLDAMTYLAA EVSGFPKERV IGQAGVLDAA
160 170 180 190 200
RYRTFIAMEA GVSVEDVQAM LMGGHGDEMV PLPRFSTISG IPVSEFIAPD
210 220 230 240 250
RLAQIVERTR KGGGEIVNLL KTGSAYYAPA AATAQMVEAV LKDKKRVMPV
260 270 280 290 300
AAYLTGQYGL NDIYFGVPVI LGAGGVEKIL ELPLNEEEMA LLNASAKAVR

ATLDTLKSL
Length:309
Mass (Da):32,751
Last modified:March 18, 2008 - v3
Checksum:iAE1BACC5D5B36331
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34F → I in CAA61436 (PubMed:8661927).Curated1
Sequence conflicti34F → I (PubMed:3133356).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89038 Genomic DNA Translation: CAA61436.1
CP000909 Genomic DNA Translation: ABY34133.1
RefSeqiWP_012256789.1, NC_010175.1
YP_001634522.1, NC_010175.1

Genome annotation databases

EnsemblBacteriaiABY34133; ABY34133; Caur_0900
GeneIDi5827960
KEGGicau:Caur_0900
PATRICifig|324602.8.peg.1031

Similar proteinsi

Entry informationi

Entry nameiMDH_CHLAA
AccessioniPrimary (citable) accession number: P80040
Secondary accession number(s): A9WH38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: March 18, 2008
Last modified: May 23, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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