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P80039

- MDH_CHLTE

UniProt

P80039 - MDH_CHLTE

Protein

Malate dehydrogenase

Gene

mdh

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (30 Aug 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321NAD1 Publication
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei94 – 941NAD1 Publication
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Active sitei174 – 1741Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 126NAD1 Publication
    Nucleotide bindingi117 – 1193NAD1 Publication

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciCTEP194439:GHN0-1546-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:CT1507
    OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
    Taxonomic identifieri194439 [NCBI]
    Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
    ProteomesiUP000001007: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Malate dehydrogenasePRO_0000113448Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; arranged as a dimer of dimers.1 Publication

    Protein-protein interaction databases

    STRINGi194439.CT1507.

    Structurei

    Secondary structure

    1
    310
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi10 – 2112
    Beta strandi26 – 316
    Beta strandi33 – 364
    Helixi37 – 4610
    Helixi49 – 524
    Beta strandi57 – 626
    Helixi64 – 674
    Beta strandi71 – 755
    Helixi92 – 10514
    Turni106 – 1083
    Beta strandi113 – 1164
    Beta strandi118 – 1203
    Helixi121 – 13212
    Helixi136 – 1383
    Beta strandi139 – 1424
    Helixi144 – 15916
    Helixi163 – 1653
    Beta strandi166 – 1727
    Helixi175 – 1773
    Beta strandi178 – 18710
    Helixi192 – 1943
    Helixi198 – 20912
    Helixi211 – 2199
    Beta strandi220 – 2223
    Helixi226 – 24015
    Beta strandi245 – 25511
    Helixi256 – 2583
    Beta strandi260 – 27112
    Beta strandi274 – 2785
    Helixi285 – 30420

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GUZX-ray2.00A/B/C/D1-71[»]
    1GV0X-ray2.50A/B1-310[»]
    ProteinModelPortaliP80039.
    SMRiP80039. Positions 1-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP80039.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213794.
    KOiK00024.
    OMAiYGQNDIC.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P80039-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKITVIGAGN VGATTAFRLA EKQLARELVL LDVVEGIPQG KALDMYESGP    50
    VGLFDTKVTG SNDYADTANS DIVVITAGLP RKPGMTREDL LSMNAGIVRE 100
    VTGRIMEHSK NPIIVVVSNP LDIMTHVAWQ KSGLPKERVI GMAGVLDSAR 150
    FRSFIAMELG VSMQDVTACV LGGHGDAMVP VVKYTTVAGI PVADLISAER 200
    IAELVERTRT GGAEIVNHLK QGSAFYAPAT SVVEMVESIV LDRKRVLTCA 250
    VSLDGQYGID GTFVGVPVKL GKNGVEHIYE IKLDQSDLDL LQKSAKIVDE 300
    NCKMLDASQG 310
    Length:310
    Mass (Da):33,092
    Last modified:August 30, 2002 - v3
    Checksum:i30A92ACFE9442A93
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti227 – 2271A → S in CAA56810. (PubMed:8955383)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80838 Genomic DNA. Translation: CAA56810.1.
    AE006470 Genomic DNA. Translation: AAM72734.1.
    RefSeqiNP_662392.1. NC_002932.3.

    Genome annotation databases

    EnsemblBacteriaiAAM72734; AAM72734; CT1507.
    GeneIDi1007223.
    KEGGicte:CT1507.
    PATRICi21400925. VBIChlTep116050_1367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80838 Genomic DNA. Translation: CAA56810.1 .
    AE006470 Genomic DNA. Translation: AAM72734.1 .
    RefSeqi NP_662392.1. NC_002932.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GUZ X-ray 2.00 A/B/C/D 1-71 [» ]
    1GV0 X-ray 2.50 A/B 1-310 [» ]
    ProteinModelPortali P80039.
    SMRi P80039. Positions 1-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 194439.CT1507.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM72734 ; AAM72734 ; CT1507 .
    GeneIDi 1007223.
    KEGGi cte:CT1507.
    PATRICi 21400925. VBIChlTep116050_1367.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213794.
    KOi K00024.
    OMAi YGQNDIC.
    OrthoDBi EOG6091FG.

    Enzyme and pathway databases

    BioCyci CTEP194439:GHN0-1546-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P80039.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Malate dehydrogenase from the mesophile Chlorobium vibrioforme and from the mild thermophile Chlorobium tepidum: molecular cloning, construction of a hybrid, and expression in Escherichia coli."
      Naterstad K., Lauvrak V., Sirevag R.
      J. Bacteriol. 178:7047-7052(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49652 / DSM 12025 / TLS.
    3. "Malate dehydrogenase from Chlorobium vibrioforme, Chlorobium tepidum, and Heliobacterium gestii: purification, characterization, and investigation of dinucleotide binding by dehydrogenases by use of empirical methods of protein sequence analysis."
      Charnock C.B., Refseth U.H., Strevaag R.
      J. Bacteriol. 174:1307-1313(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-34.
    4. "Structural basis for thermophilic protein stability: structures of thermophilic and mesophilic malate dehydrogenases."
      Dalhus B., Saarinen M., Sauer U.H., Eklund P., Johansson K., Karlsson A., Ramaswamy S., Bjoerk A., Synstad B., Naterstad K., Sirevaag R., Eklund H.
      J. Mol. Biol. 318:707-721(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NAD, SUBUNIT.

    Entry informationi

    Entry nameiMDH_CHLTE
    AccessioniPrimary (citable) accession number: P80039
    Secondary accession number(s): P94677
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: August 30, 2002
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3