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Protein

Malate dehydrogenase

Gene

mdh

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32NADUniRule annotation1 Publication1
Binding sitei81SubstrateUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei94NADUniRule annotation1 Publication1
Binding sitei119SubstrateUniRule annotation1
Binding sitei150SubstrateUniRule annotation1
Active sitei174Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADUniRule annotation1 Publication6
Nucleotide bindingi117 – 119NADUniRule annotation1 Publication3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:CT1507
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134481 – 310Malate dehydrogenaseAdd BLAST310

Interactioni

Subunit structurei

Homotetramer; arranged as a dimer of dimers.1 Publication

Protein-protein interaction databases

STRINGi194439.CT1507.

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 21Combined sources12
Beta strandi26 – 31Combined sources6
Beta strandi33 – 36Combined sources4
Helixi37 – 46Combined sources10
Helixi49 – 52Combined sources4
Beta strandi57 – 62Combined sources6
Helixi64 – 67Combined sources4
Beta strandi71 – 75Combined sources5
Helixi92 – 105Combined sources14
Turni106 – 108Combined sources3
Beta strandi113 – 116Combined sources4
Beta strandi118 – 120Combined sources3
Helixi121 – 132Combined sources12
Helixi136 – 138Combined sources3
Beta strandi139 – 142Combined sources4
Helixi144 – 159Combined sources16
Helixi163 – 165Combined sources3
Beta strandi166 – 172Combined sources7
Helixi175 – 177Combined sources3
Beta strandi178 – 187Combined sources10
Helixi192 – 194Combined sources3
Helixi198 – 209Combined sources12
Helixi211 – 219Combined sources9
Beta strandi220 – 222Combined sources3
Helixi226 – 240Combined sources15
Beta strandi245 – 255Combined sources11
Helixi256 – 258Combined sources3
Beta strandi260 – 271Combined sources12
Beta strandi274 – 278Combined sources5
Helixi285 – 304Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUZX-ray2.00A/B/C/D1-71[»]
1GV0X-ray2.50A/B1-310[»]
ProteinModelPortaliP80039.
SMRiP80039.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80039.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P80039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITVIGAGN VGATTAFRLA EKQLARELVL LDVVEGIPQG KALDMYESGP
60 70 80 90 100
VGLFDTKVTG SNDYADTANS DIVVITAGLP RKPGMTREDL LSMNAGIVRE
110 120 130 140 150
VTGRIMEHSK NPIIVVVSNP LDIMTHVAWQ KSGLPKERVI GMAGVLDSAR
160 170 180 190 200
FRSFIAMELG VSMQDVTACV LGGHGDAMVP VVKYTTVAGI PVADLISAER
210 220 230 240 250
IAELVERTRT GGAEIVNHLK QGSAFYAPAT SVVEMVESIV LDRKRVLTCA
260 270 280 290 300
VSLDGQYGID GTFVGVPVKL GKNGVEHIYE IKLDQSDLDL LQKSAKIVDE
310
NCKMLDASQG
Length:310
Mass (Da):33,092
Last modified:August 30, 2002 - v3
Checksum:i30A92ACFE9442A93
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti227A → S in CAA56810 (PubMed:8955383).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80838 Genomic DNA. Translation: CAA56810.1.
AE006470 Genomic DNA. Translation: AAM72734.1.
RefSeqiNP_662392.1. NC_002932.3.
WP_010933173.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM72734; AAM72734; CT1507.
GeneIDi1007223.
KEGGicte:CT1507.
PATRICi21400925. VBIChlTep116050_1367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80838 Genomic DNA. Translation: CAA56810.1.
AE006470 Genomic DNA. Translation: AAM72734.1.
RefSeqiNP_662392.1. NC_002932.3.
WP_010933173.1. NC_002932.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GUZX-ray2.00A/B/C/D1-71[»]
1GV0X-ray2.50A/B1-310[»]
ProteinModelPortaliP80039.
SMRiP80039.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT1507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM72734; AAM72734; CT1507.
GeneIDi1007223.
KEGGicte:CT1507.
PATRICi21400925. VBIChlTep116050_1367.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiMDLMQTA.
OrthoDBiPOG091H02N6.

Miscellaneous databases

EvolutionaryTraceiP80039.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_CHLTE
AccessioniPrimary (citable) accession number: P80039
Secondary accession number(s): P94677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: August 30, 2002
Last modified: November 30, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.