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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Lactobacillus delbrueckii subsp. bulgaricus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation1 Publication

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate. The binding affinities for these effectors are decreased however, and therefore the allosteric effect becomes apparent only at high effector concentrations.UniRule annotation2 Publications

Kineticsi

  1. KM=0.2 mM for ATP (at pH 8.2)1 Publication
  2. KM=0.07 mM for ATP (at pH 6.0)1 Publication
  3. KM=0.3 mM for fructose 6-phosphate (at pH 6.0 and 8.2)1 Publication
  1. Vmax=90 µmol/min/mg enzyme (at pH 8.2)1 Publication

pH dependencei

Optimum pH is 8.2.1 Publication

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. 6-phosphofructokinase (CFL1_01460), ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA), 6-phosphofructokinase (AT236_01721)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11ATP; via amide nitrogenUniRule annotation1
Metal bindingi103Magnesium; catalyticUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei154Allosteric activator ADPUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei243Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi102 – 105ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP80019.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
OrganismiLactobacillus delbrueckii subsp. bulgaricus
Taxonomic identifieri1585 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119571 – 319ATP-dependent 6-phosphofructokinaseAdd BLAST319

Interactioni

Subunit structurei

Homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi390333.Ldb0838.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi16 – 28Combined sources13
Turni29 – 31Combined sources3
Beta strandi33 – 37Combined sources5
Helixi40 – 46Combined sources7
Beta strandi49 – 51Combined sources3
Helixi54 – 57Combined sources4
Helixi74 – 76Combined sources3
Helixi79 – 91Combined sources13
Beta strandi96 – 101Combined sources6
Helixi103 – 114Combined sources12
Beta strandi119 – 125Combined sources7
Helixi139 – 159Combined sources21
Beta strandi163 – 168Combined sources6
Helixi175 – 183Combined sources9
Beta strandi187 – 190Combined sources4
Helixi198 – 210Combined sources13
Beta strandi216 – 221Combined sources6
Turni222 – 224Combined sources3
Helixi227 – 236Combined sources10
Beta strandi242 – 246Combined sources5
Helixi248 – 252Combined sources5
Helixi258 – 276Combined sources19
Beta strandi281 – 287Combined sources7
Beta strandi290 – 295Combined sources6
Helixi296 – 299Combined sources4
Helixi309 – 317Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZXXX-ray1.85A1-319[»]
ProteinModelPortaliP80019.
SMRiP80019.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80019.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 25Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni185 – 187Allosteric activator ADP bindingUniRule annotation3
Regioni213 – 215Allosteric activator ADP bindingUniRule annotation3
Regioni249 – 252Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.

Family and domain databases

CDDicd00763. Bacterial_PFK. 1 hit.
HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIGILTSG GDAPGMNAAV RAVTRVAIAN GLEVFGIRYG FAGLVAGDIF
60 70 80 90 100
PLESEDVAHL INVSGTFLYS ARYPEFAEEE GQLAGIEQLK KHGIDAVVVI
110 120 130 140 150
GGDGSYHGAL QLTRHGFNSI GLPGTIDNDI PYTDATIGYD TACMTAMDAI
160 170 180 190 200
DKIRDTASSH HRVFIVNVMG RNCGDIAMRV GVACGADAIV IPERPYDVEE
210 220 230 240 250
IANRLKQAQE SGKDHGLVVV AEGVMTADQF MAELKKYGDF DVRANVLGHM
260 270 280 290 300
QRGGTPTVSD RVLASKLGSE AVHLLLEGKG GLAVGIENGK VTSHDILDLF
310
DESHRGDYDL LKLNADLSR
Length:319
Mass (Da):34,009
Last modified:June 1, 1994 - v2
Checksum:i555C5FE1D522BC9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71403 Genomic DNA. Translation: CAA50526.1.
PIRiA48663. S35928.
RefSeqiWP_003619813.1. NZ_LVWY01000013.1.

Genome annotation databases

GeneIDi4085048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71403 Genomic DNA. Translation: CAA50526.1.
PIRiA48663. S35928.
RefSeqiWP_003619813.1. NZ_LVWY01000013.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZXXX-ray1.85A1-319[»]
ProteinModelPortaliP80019.
SMRiP80019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390333.Ldb0838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4085048.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
SABIO-RKP80019.

Miscellaneous databases

EvolutionaryTraceiP80019.

Family and domain databases

CDDicd00763. Bacterial_PFK. 1 hit.
HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_LACDE
AccessioniPrimary (citable) accession number: P80019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.