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Protein

Plasminogen

Gene

PLG

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).By similarity

Miscellaneous

Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.

Catalytic activityi

Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.

Enzyme regulationi

Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Activated with urokinase and high concentrations of streptokinase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145Charge relay systemBy similarity1
Active sitei188Charge relay systemBy similarity1
Sitei277Site of substrate specificityBy similarity1
Active sitei283Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processBlood coagulation, Fibrinolysis, Hemostasis, Tissue remodeling

Protein family/group databases

MEROPSiS01.233.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen (EC:3.4.21.7)
Cleaved into the following 2 chains:
Gene namesi
Name:PLG
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000028044‹1 – 103Plasmin heavy chain AAdd BLAST›103
ChainiPRO_0000028045104 – 333Plasmin light chain BAdd BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi4 ↔ 83By similarity
Disulfide bondi25 ↔ 66By similarity
Disulfide bondi54 ↔ 78By similarity
Disulfide bondi90 ↔ 208Interchain (between A and B chains)By similarity
Disulfide bondi100 ↔ 108Interchain (between A and B chains)By similarity
Modified residuei120PhosphoserineBy similarity1
Disulfide bondi130 ↔ 146By similarity
Disulfide bondi222 ↔ 289By similarity
Disulfide bondi252 ↔ 268By similarity
Disulfide bondi279 ↔ 307By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP80009.
PRIDEiP80009.

Interactioni

Subunit structurei

Interacts with CSPG4 and AMOT. Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation. Interacts (via Kringle 4 domain) with ADA; the interaction stimulates PLG activation when in complex with DPP4. Angiostatin: Interacts with ATP5A1; the interaction inhibits most of the angiogenic effects of angiostatin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei152Interaction with streptokinaseCurated1
Sitei186Interaction with streptokinaseCurated1
Sitei264Interaction with streptokinaseCurated1

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001078.

Structurei

3D structure databases

ProteinModelPortaliP80009.
SMRiP80009.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 83Kringle 5PROSITE-ProRule annotationAdd BLAST80
Domaini104 – 331Peptidase S1PROSITE-ProRule annotationAdd BLAST228

Domaini

Kringle domains mediate interaction with CSPG4.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasminogen subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Kringle

Phylogenomic databases

eggNOGiENOG410IDXR. Eukaryota.
COG5640. LUCA.
HOVERGENiHBG008633.
InParanoidiP80009.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiView protein in InterPro
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
PfamiView protein in Pfam
PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiView protein in SMART
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiView protein in PROSITE
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ASDCMFGNGK GYRGKKATTV MGIPCQEWAA QEPHRHSIFT PETNPQAGLE
60 70 80 90 100
KNYCRNPDGD VNGPWCYTMN QRKLFDYCDV PQCVSTSFDC GKPQVEPKKC
110 120 130 140 150
PGRVVGGCVA NPHSWPWQIS LRTRYGKHFC GGTLISPEWV LTAAHCLERS
160 170 180 190 200
SRPASYKVIL GAHKEVNLES DVQEIEVYKL FLEPTRADIA LLKLSSPAVI
210 220 230 240 250
TSKVIPACLP PPNYVVADRT LCYITGWGET QGTYGAGLLK EAQLPVIENK
260 270 280 290 300
VCNRYEYLNG RVKSTELCAG NLAGGTDSCQ GDSGGPLVCF EKDKYILQGV
310 320 330
TSWGLGCARP NKPGVYVRVS RFVTWIEGIM RNN
Length:333
Mass (Da):36,678
Last modified:November 1, 1991 - v1
Checksum:iC8C0271B6C6AC8D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

UniGeneiCfa.3464.

Similar proteinsi

Entry informationi

Entry nameiPLMN_CANLF
AccessioniPrimary (citable) accession number: P80009
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 15, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families