P80009 (PLMN_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plasminogen
EC=3.4.21.7

Cleaved into the following 2 chains:

1. Plasmin heavy chain A
2. Plasmin light chain B

Gene names

Name:

PLG

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	333 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells By similarity.
Catalytic activity	Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products.
Enzyme regulation	Converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. Activated with urokinase and high concentrations of streptokinase.
Subunit structure	Interacts with CSPG4 and AMOT. Interacts (via the Kringle domains) with HRG; the interaction tethers PLG to the cell surface and enhances its activation By similarity.
Subcellular location	Secreted By similarity. Note: Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface By similarity.
Domain	Kringle domains mediate interaction with CSPG4 By similarity.
Miscellaneous	Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot.
Sequence similarities	Belongs to the peptidase S1 family. Plasminogen subfamily. Contains at least 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Blood coagulation Fibrinolysis Tissue remodeling
Cellular component	Secreted
Domain	Kringle
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW fibrinolysis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW tissue remodeling Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 103	›103	Plasmin heavy chain A		PRO_0000028044
	Chain	104 – 333	230	Plasmin light chain B		PRO_0000028045
Regions
	Domain	4 – 83	80	Kringle 5
	Domain	104 – 331	228	Peptidase S1
Sites
	Active site	145	1	Charge relay system By similarity
	Active site	188	1	Charge relay system By similarity
	Active site	283	1	Charge relay system By similarity
	Site	152	1	Interaction with streptokinase Probable
	Site	186	1	Interaction with streptokinase Probable
	Site	264	1	Interaction with streptokinase Probable
	Site	277	1	Site of substrate specificity By similarity
Amino acid modifications
	Disulfide bond	4 ↔ 83		By similarity
	Disulfide bond	25 ↔ 66		By similarity
	Disulfide bond	54 ↔ 78		By similarity
	Disulfide bond	90 ↔ 208		Interchain (between A and B chains) By similarity
	Disulfide bond	100 ↔ 108		Interchain (between A and B chains) By similarity
	Disulfide bond	130 ↔ 146		By similarity
	Disulfide bond	222 ↔ 289		By similarity
	Disulfide bond	252 ↔ 268		By similarity
	Disulfide bond	279 ↔ 307		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P80009 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: C8C0271B6C6AC8D4	FASTA	333	36,678
	10 20 30 40 50 60 ASDCMFGNGK GYRGKKATTV MGIPCQEWAA QEPHRHSIFT PETNPQAGLE KNYCRNPDGD 70 80 90 100 110 120 VNGPWCYTMN QRKLFDYCDV PQCVSTSFDC GKPQVEPKKC PGRVVGGCVA NPHSWPWQIS 130 140 150 160 170 180 LRTRYGKHFC GGTLISPEWV LTAAHCLERS SRPASYKVIL GAHKEVNLES DVQEIEVYKL 190 200 210 220 230 240 FLEPTRADIA LLKLSSPAVI TSKVIPACLP PPNYVVADRT LCYITGWGET QGTYGAGLLK 250 260 270 280 290 300 EAQLPVIENK VCNRYEYLNG RVKSTELCAG NLAGGTDSCQ GDSGGPLVCF EKDKYILQGV 310 320 330 TSWGLGCARP NKPGVYVRVS RFVTWIEGIM RNN

References

[1]	"Complete amino acid sequence of canine miniplasminogen." Schaller J., Straub C., Kaempfer U., Rickli E.E. Protein Seq. Data Anal. 2:445-450(1989) [PubMed: 2626424] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

3D structure databases
ProteinModelPortal	P80009.
SMR	P80009. Positions 3-85, 87-333.
ModBase	Search...
Protein-protein interaction databases
STRING	P80009.
Protein family/group databases
MEROPS	S01.233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	maNOG09109.
HOVERGEN	HBG008633.
InParanoid	P80009.
OrthoDB	EOG4RR6GQ.
Family and domain databases
InterPro	IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
SMART	SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF57440. Kringle-like. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLMN_CANFA

Accession

Primary (citable) accession number: P80009

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program