P80003 (PA2A2_HELSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Acidic phospholipase A2 PA4 Short name=PLA2 EC=3.1.1.4 Alternative name(s): Phosphatidylcholine 2-acylhydrolase Cleaved into the following chain: Acidic phospholipase A2 PA2
Organism	Heloderma suspectum (Gila monster)
Taxonomic identifier	8554 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Anguimorpha › Helodermatidae › Heloderma

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group III subfamily.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 142	142	Acidic phospholipase A2 PA4		PRO_0000045885
	Chain	1 – 141	141	Acidic phospholipase A2 PA2		PRO_0000045886
Sites
	Active site	36	1	By similarity
	Metal binding	10	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	12	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	14	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	37	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 33		By similarity
	Disulfide bond	32 ↔ 72		By similarity
	Disulfide bond	39 ↔ 65		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80003 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 9B79D5F5CD775E3B	FASTA	142	15,640
	10 20 30 40 50 60 GAFIMPGTLW CGAGNAASDY SQLGTEKDTD MCCRDHDHCS DTMAALEYKH GMRNYRPHTV 70 80 90 100 110 120 SHCDCDNQFR SCLMNVKDRT ADLVGMTYFT VLKISCFELE EGEGCVDNNF SQQCTKSEIM 130 140 PVAKLVSAAP YQAQAETQSG EG

References

[1]	"Differences in primary structure among five phospholipases A2 from Heloderma suspectum." Vandermeers A., Vandermeers-Piret M.-C., Vigneron L., Rathe J., Stievenart M., Christophe J. Eur. J. Biochem. 196:537-544(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	S25093.
3D structure databases
ProteinModelPortal	P80003.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008138.
Family and domain databases
Gene3D	1.20.90.10. 1 hit.
InterPro	IPR001211. PLipase_A2. IPR013090. PLipase_A2_AS. IPR016090. PLipase_A2_dom. [Graphical view]
PANTHER	PTHR12253. PTHR12253. 1 hit.
Pfam	PF05826. Phospholip_A2_2. 1 hit. [Graphical view]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. False negative. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA2A2_HELSU

Accession

Primary (citable) accession number: P80003
Secondary accession number(s): P80006

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program