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Reviewed, UniProtKB/Swiss-Prot P80003 (PA22_HELSU)

Last modified November 25, 2008. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phospholipase A2 isozyme PA4
    EC=3.1.1.4
Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
Cleaved into the following chain:
    1- Recommended name:
            Phospholipase A2 isozyme PA2
OrganismHeloderma suspectum (Gila monster)
Taxonomic identifier8554 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataScleroglossaAnguimorphaHelodermatidaeHeloderma

Protein attributes

Sequence length142 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activity

Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Sequence similarities

Belongs to the phospholipase A2 family.

Ontologies

Keywords

   Biological processLipid degradation
   Cellular componentSecreted
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Biological processlipid catabolic process

Inferred from electronic annotation. Source: InterPro

phospholipid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

phospholipase A2 activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 142142Phospholipase A2 isozyme PA4
PRO_0000045885
Chain1 – 141141Phospholipase A2 isozyme PA2
PRO_0000045886

Sites

Active site361 By similarity
Metal binding101Calcium; via carbonyl oxygen By similarity
Metal binding121Calcium; via carbonyl oxygen By similarity
Metal binding141Calcium; via carbonyl oxygen By similarity
Metal binding371Calcium By similarity

Amino acid modifications

Disulfide bond11 ↔ 33 By similarity
Disulfide bond32 ↔ 72 By similarity
Disulfide bond39 ↔ 65 By similarity

Sequences

Sequence LengthMass (Da)Tools
P80003-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 9B79D5F5CD775E3B

FASTA14215,640
        10         20         30         40         50         60 
GAFIMPGTLW CGAGNAASDY SQLGTEKDTD MCCRDHDHCS DTMAALEYKH GMRNYRPHTV 

        70         80         90        100        110        120 
SHCDCDNQFR SCLMNVKDRT ADLVGMTYFT VLKISCFELE EGEGCVDNNF SQQCTKSEIM 

       130        140 
PVAKLVSAAP YQAQAETQSG EG 

« Hide

References

[1]"Differences in primary structure among five phospholipases A2 from Heloderma suspectum."
Vandermeers A., Vandermeers-Piret M.-C., Vigneron L., Rathe J., Stievenart M., Christophe J.
Eur. J. Biochem. 196:537-544(1991) [PubMed: 2013276] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Venom.

Cross-references

Sequence databases

PIRS25093.

3D structure databases

HSSPHSSP built from PDB template 1POC based on UniProtKB P00630.
ModBaseSearch...

Phylogenomic databases

HOVERGENP80003.

Family and domain databases

InterProIPR016090. Phospholipase_A2.
IPR013090. Phospholipase_A2_AS.
IPR001211. Phospholipase_A2_euk.
IPR008774. Phospholipase_A2_met.
[Graphical view]
Gene3DG3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHERPTHR12253. Phospholipase_A2_met. 1 hit.
PfamPF05826. Phospholip_A2_2. 1 hit.
[Graphical view]
SMARTSM00085. PA2c. 1 hit.
[Graphical view]
PROSITEPS00119. PA2_ASP. False negative.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePA22_HELSU
AccessionPrimary (citable) accession number: P80003
Secondary accession number(s): P80006
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1995
Last modified: November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents