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Protein

Wilms tumor protein homolog B

Gene

wt1-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor required for development of the vascular component of the pronephric kidney, the glomus; may repress tubule-specific gene expression in the portion of the pronephros fated to form the glomus (PubMed:16818449). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (By similarity). Inhibits Wnt-signaling during embryonic development (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei382Important for interaction with target DNABy similarity1
Sitei388Important for interaction with target DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri284 – 308C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri314 – 338C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri344 – 366C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri372 – 396C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • glomus development Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of Wnt signaling pathway Source: UniProtKB
  • pronephros development Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Wilms tumor protein homolog B
Short name:
XWT1b1 Publication
Short name:
XeWT11 Publication
Gene namesi
Name:wt1-b
Synonyms:wt1Imported
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252376. wt1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Nucleus speckle By similarity

  • Note: Shuttles between nucleus and cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003913881 – 407Wilms tumor protein homolog BAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki158Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Expressioni

Tissue specificityi

Expressed in the pronephric anlage from stage 23 to 30. Also expressed in the adult kidney (mesonephros) and in testis.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP79958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni328 – 342Important for interaction with target DNABy similarityAdd BLAST15
Regioni354 – 362Important for interaction with target DNABy similarity9

Domaini

Binds to DNA motifs with the sequence 5'-GCG(T/G)GGGCG-3' via its C2H2-type zinc fingers. Starting from the N-terminus, the second zinc finger binds to the 3'-GCG motif, the middle zinc finger interacts with the central TGG motif, and the C-terminal zinc finger binds to the 5'-GCG motif. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Has reduced affinity for target DNA where the cytosines have been oxidized to 5-hydroxymethylcytosine, 5-formylcytosine or 5-carboxylcytosine.By similarity

Sequence similaritiesi

Belongs to the EGR C2H2-type zinc-finger protein family.Sequence analysis
Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri284 – 308C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri314 – 338C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri344 – 366C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri372 – 396C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG006960.
KOiK09234.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017987. Wilms_tumour.
IPR000976. Wilms_tumour_N.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02165. WT1. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
PRINTSiPR00049. WILMSTUMOUR.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P79958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSDVRDMNL LPPVSSLSGN SSCNMPVSNS SQWAPVLDFP PGAPYSSLTP
60 70 80 90 100
HSFIKQEPTW NPDPHEDQCL SAFTVHFSGQ FTGTAGACRY GPFGAPTPSQ
110 120 130 140 150
ATTGQARMFP NAPYLSNCLD NQQSMRNQGY SAVAFDGTPS YGHTPSHHTA
160 170 180 190 200
QFTNHSFKHE DPISQQTSLG EQQYSVPPPV YGCHTPTDTC TGSQALLLRT
210 220 230 240 250
PYNSDNLYQM ECMTWNQMNL GSSLKSHGTT YENDSHSAPM LYSCGGQYRI
260 270 280 290 300
HTHGVFRGIQ DVRRVPGVTP AIVRSTEANE KRPFMCAYPG CNKRYFKLSH
310 320 330 340 350
LQMHSRKHTG EKPYQCDFKD CERRFSRSDQ LKRHQRRHTG VKPFQCKTCQ
360 370 380 390 400
RKFSRSDHLK THTRTHTGEK PFSCRWPSCQ KKFARSDELV RHHNMHQRNM

TKLQLAL
Length:407
Mass (Da):45,983
Last modified:May 1, 1997 - v1
Checksum:iE2554C658005870C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82051 mRNA. Translation: BAA11522.1.
PIRiJC5046.
RefSeqiNP_001079336.1. NM_001085867.1.
UniGeneiXl.246.

Genome annotation databases

GeneIDi378664.
KEGGixla:378664.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82051 mRNA. Translation: BAA11522.1.
PIRiJC5046.
RefSeqiNP_001079336.1. NM_001085867.1.
UniGeneiXl.246.

3D structure databases

ProteinModelPortaliP79958.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi378664.
KEGGixla:378664.

Organism-specific databases

CTDi378664.
XenbaseiXB-GENE-6252376. wt1.

Phylogenomic databases

HOVERGENiHBG006960.
KOiK09234.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR017987. Wilms_tumour.
IPR000976. Wilms_tumour_N.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF02165. WT1. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
PRINTSiPR00049. WILMSTUMOUR.
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWT1B_XENLA
AccessioniPrimary (citable) accession number: P79958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.