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Protein

Guanylyl cyclase-activating protein 1

Gene

GUCA1A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of retinal guanylyl cyclase is a key event in recovery of the dark state of rod photoreceptors following light exposure.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi63 – 741211 PublicationAdd
BLAST
Calcium bindingi99 – 1101221 PublicationAdd
BLAST
Calcium bindingi142 – 1531231 PublicationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-GGA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylyl cyclase-activating protein 1
Short name:
GCAP 1
Alternative name(s):
Guanylate cyclase activator 1A
Gene namesi
Name:GUCA1A
Synonyms:GCAP1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 199198Guanylyl cyclase-activating protein 1PRO_0000073805Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Modified residuei3 – 31Deamidated asparagineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP79880.

Expressioni

Tissue specificityi

Retina, in rod and cone outer segments, and pineal gland.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000002177.

Structurei

Secondary structure

1
199
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 138Combined sources
Helixi17 – 2711Combined sources
Beta strandi31 – 333Combined sources
Helixi35 – 428Combined sources
Helixi49 – 6214Combined sources
Helixi72 – 8211Combined sources
Helixi87 – 9812Combined sources
Helixi108 – 11710Combined sources
Helixi120 – 1234Combined sources
Beta strandi124 – 1263Combined sources
Helixi130 – 14112Combined sources
Beta strandi146 – 1494Combined sources
Helixi151 – 1588Combined sources
Helixi162 – 1698Combined sources
Helixi174 – 1818Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R2IX-ray2.00A2-199[»]
ProteinModelPortaliP79880.
SMRiP79880. Positions 2-185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP79880.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini50 – 8536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini86 – 12136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini129 – 16436EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Domaini

Binds three calcium ions (via EF-hands 2, 3 and 4) when calcium levels are high (PubMed:17997965). Binds Mg2+ when calcium levels are low.By similarity1 Publication

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP79880.
KOiK08328.
OMAiINPCSDS.
OrthoDBiEOG7BP83V.
PhylomeDBiP79880.
TreeFamiTF333971.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR033022. GUCA1A.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF13. PTHR23055:SF13. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNMDGKAVE ELSATECHQW YKKFMTECPS GQLTLYEFKQ FFGLKNLSPS
60 70 80 90 100
ANKYVEQMFE TFDFNKDGYI DFMEYVAALS LVLKGKVDQK LRWYFKLYDV
110 120 130 140 150
DGNGCIDRGE LLNIIKAIRA INRCNEAMTA EEFTNMVFDK IDINGDGELS
160 170 180 190
LEEFMEGVQK DEVLLDILTR SLDLTHIVKL IQNDGKNPHA PEEAEEAAQ
Length:199
Mass (Da):22,808
Last modified:January 23, 2007 - v3
Checksum:i2F4ED65CF471F9F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82199 mRNA. Translation: AAB47111.1.
AF172707 Genomic DNA. Translation: AAD47879.1.
PIRiS68838.
RefSeqiNP_989651.1. NM_204320.2.
UniGeneiGga.625.

Genome annotation databases

EnsembliENSGALT00000002179; ENSGALP00000002177; ENSGALG00000001431.
ENSGALT00000028393; ENSGALP00000028327; ENSGALG00000001431.
GeneIDi374216.
KEGGigga:374216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82199 mRNA. Translation: AAB47111.1.
AF172707 Genomic DNA. Translation: AAD47879.1.
PIRiS68838.
RefSeqiNP_989651.1. NM_204320.2.
UniGeneiGga.625.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R2IX-ray2.00A2-199[»]
ProteinModelPortaliP79880.
SMRiP79880. Positions 2-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000002177.

Proteomic databases

PaxDbiP79880.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000002179; ENSGALP00000002177; ENSGALG00000001431.
ENSGALT00000028393; ENSGALP00000028327; ENSGALG00000001431.
GeneIDi374216.
KEGGigga:374216.

Organism-specific databases

CTDi2978.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP79880.
KOiK08328.
OMAiINPCSDS.
OrthoDBiEOG7BP83V.
PhylomeDBiP79880.
TreeFamiTF333971.

Enzyme and pathway databases

ReactomeiR-GGA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

EvolutionaryTraceiP79880.
PROiP79880.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR033022. GUCA1A.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF13. PTHR23055:SF13. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of GCAP1 and GCAP2 in the retinal degeneration (rd) mutant chicken retina."
    Semple-Rowland S.L., Gorczyca W.A., Buczylko J., Helekar B.S., Ruiz C.C., Subbaraya I., Palczewski K., Baehr W.
    FEBS Lett. 385:47-52(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "Characterization of the chicken GCAP gene array and analyses of GCAP1, GCAP2, and GC1 gene expression in normal and rd chicken pineal."
    Semple-Rowland S.L., Larkin P., Bronson J.D., Nykamp K., Streit W.J., Baehr W.
    Mol. Vis. 5:14-14(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: White leghorn.
    Tissue: Liver.
  3. "Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1."
    Stephen R., Bereta G., Golczak M., Palczewski K., Sousa M.C.
    Structure 15:1392-1402(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-199, CALCIUM-BINDING, MYRISTOYLATION AT GLY-2, DOMAIN.

Entry informationi

Entry nameiGUC1A_CHICK
AccessioniPrimary (citable) accession number: P79880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.