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Protein

Aryl hydrocarbon receptor nuclear translocator

Gene

arnt

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1 specifically recognizes the xenobiotic response element (XRE), functions positively in AHR-mediated signaling and activates transcription. Isoform 2 has reduced affinity for the XRE and functions negatively in AHR-mediated signaling by disrupting the binding of dimers formed by isoform 1 and AHR to the XRE.2 Publications

GO - Molecular functioni

  • aryl hydrocarbon receptor binding Source: UniProtKB
  • DNA binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • negative regulation of signal transduction Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of signal transduction Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aryl hydrocarbon receptor nuclear translocator
Short name:
rtARNT
Gene namesi
Name:arnt
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation2 Publications

GO - Cellular componenti

  • cytoplasm Source: InterPro
  • nucleus Source: UniProtKB
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723Aryl hydrocarbon receptor nuclear translocatorPRO_0000245792Add
BLAST

Expressioni

Tissue specificityi

In day 23 embryos, expressed at highest levels in brain, spinal cord and epithelial cells of developing gill. Expressed at higher levels throughout all tissues in day 27 and 35 sac fry, predominantly in brain and spinal cord. In the adult, isoform 1 is widely expressed (at protein level) and is detected in liver, gill, gonad, brain, kidney, heart, spleen and muscle. Isoform 2 is expressed (at protein level) only in gills with highest expression in the epithelial cells sourrounding the gill lamellae.1 Publication

Developmental stagei

Isoform 1 is expressed both in juvenile and adult with highest levels in day 42 sac fry. Isoform 2 is only expressed in adult (at protein level).1 Publication

Interactioni

Subunit structurei

Efficient DNA-binding requires dimerization with another bHLH protein. Heterodimer with the aryl hydrocarbon receptor (AHR).

Binary interactionsi

WithEntry#Exp.IntActNotes
AhrP305612EBI-958635,EBI-78863From a different organism.

GO - Molecular functioni

  • aryl hydrocarbon receptor binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

IntActiP79832. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP79832.
SMRiP79832. Positions 330-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 11854bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini137 – 20872PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini325 – 39571PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini400 – 44344PACSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi40 – 467Poly-AspSequence analysis
Compositional biasi442 – 4476Poly-GlnSequence analysis

Domaini

The C-terminal region of isoform 1 contains a transactivation domain. The Pro/Ser/Thr-rich region in the C-terminus of isoform 2 inhibits DNA-binding of ARNT-AHR dimers.1 Publication

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.Sequence analysis
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG000164.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR001067. Nuc_translocat.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00785. NCTRNSLOCATR.
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 4 hits.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: P79832-1) [UniParc]FASTAAdd to basket

Also known as: rtARNTb1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSSIPDIPD DSLGLGAGGA QASSSACCAK RVNKRRAAPD FDDDDDGSKL
60 70 80 90 100
FRCDDDGGGG DKERFARENH SEIERRRRNK MTAYITELSD MVPTCSALAR
110 120 130 140 150
KPDKLTILRM AVSHMKSLRG SGNTAADGTY KPSFLTDQEL KHLILEAADG
160 170 180 190 200
FLFVVSCESG RVVYVSDSLT PVLNQSQSDW LGSSLYDQLH PDDGDKLREQ
210 220 230 240 250
LSTAESNNTG RMLDLKTGTV KKEGQQSSVR MCMGARRSFI CRMRCGSCPV
260 270 280 290 300
EPMSMNRLNF LRSRNRNGLG PPKDGEPQYV VVHCTGYIKS WPPTGVNLTD
310 320 330 340 350
EEADNILGSR YCLVAIGRLQ VTSCPSDTDM NSISVPVEFI SRHNCQGLFT
360 370 380 390 400
FVDHRCMATV GYQPQELLGK NILELAHPED QELLRDSFQQ VVKLKGQVLS
410 420 430 440 450
VMFRFLSKTR DWLWIRTSSF TFQNPFSEEI EYIICTNANV KQLQQQQAEL
460 470 480 490 500
GGGGRDGLYE AGQVTLPQMP VQAVTAAGTD HSKTMDKAEM HPSMYPNPDQ
510 520 530 540 550
AKFLPSTSAP GVPIYPQDNN NYTTANRSND TYSRSVGMAP QMVQPSHSAG
560 570 580 590 600
QVLAQMSRQN GAPPSNSSPL QGGAAVSWPG PAAGARPPFN NQQVVPQAGK
610 620 630 640 650
ALSPQFAMGS FVGGSSSSFG AMPTTAAPTP TMGANYPNIN PRATLNTNGY
660 670 680 690 700
DGLGSGQQFP SRAVEAVWPQ WQGQQQAQNR AEQHPHTQNN QPDIFPDVLA
710 720
MLDQPANFNN DDFEIPIYPS FNE
Length:723
Mass (Da):79,047
Last modified:May 1, 1997 - v1
Checksum:i83F6EBE711EDEB82
GO
Isoform 21 Publication (identifier: P79832-2) [UniParc]FASTAAdd to basket

Also known as: rtARNTa1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     534-723: RSVGMAPQMV...EIPIYPSFNE → SSPPGQWRQC...LPVVIQREHV

Note: Expressed at much lower levels than isoform 1.1 Publication
Show »
Length:637
Mass (Da):70,115
Checksum:iEDD3D0824A8E3B90
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti521 – 5211Missing in AAC60051 (PubMed:8940073).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei534 – 723190RSVGM…PSFNE → SSPPGQWRQCGPSGRASSKL RTGQSSTPTHRTTSLTSSLM FSPCWTSQPTSTTMTLRFPS TPLSTSDLTYTLPLTSSVSQ MSPSLSFGALCSLSLPVVIQ REHV in isoform 2. 1 PublicationVSP_052086Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73840 mRNA. Translation: AAC60051.1.
U73841 mRNA. Translation: AAC60052.1.
RefSeqiNP_001118182.1. NM_001124710.1. [P79832-1]
UniGeneiOmy.14654.

Genome annotation databases

GeneIDi100136759.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73840 mRNA. Translation: AAC60051.1.
U73841 mRNA. Translation: AAC60052.1.
RefSeqiNP_001118182.1. NM_001124710.1. [P79832-1]
UniGeneiOmy.14654.

3D structure databases

ProteinModelPortaliP79832.
SMRiP79832. Positions 330-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP79832. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100136759.

Organism-specific databases

CTDi405.

Phylogenomic databases

HOVERGENiHBG000164.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR001067. Nuc_translocat.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00785. NCTRNSLOCATR.
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 4 hits.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and expression of cDNAs from rainbow trout (Oncorhynchus mykiss) that encode two novel basic helix-loop-helix/PER-ARNT-SIM (bHLH/PAS) proteins with distinct functions in the presence of the aryl hydrocarbon receptor. Evidence for alternative mRNA splicing and dominant negative activity in the bHLH/PAS family."
    Pollenz R.S., Sullivan H.R., Holmes J., Necela B., Peterson R.E.
    J. Biol. Chem. 271:30886-30896(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH AHR, SUBCELLULAR LOCATION.
    Tissue: Gonad1 Publication.
  2. "Functional analysis of activation and repression domains of the rainbow trout aryl hydrocarbon receptor nuclear translocator (rtARNT) protein isoforms."
    Necela B., Pollenz R.S.
    Biochem. Pharmacol. 57:1177-1190(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.
  3. "Expression of aryl hydrocarbon receptor nuclear translocator (ARNT) isoforms in juvenile and adult rainbow trout tissues."
    Sojka K.M., Pollenz R.S.
    Mar. Biotechnol. 3:416-427(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiARNT_ONCMY
AccessioniPrimary (citable) accession number: P79832
Secondary accession number(s): P79831
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: May 1, 1997
Last modified: October 14, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.