P79812 (OPSD_NEOSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhodopsin

Gene names

Name:

rho

Organism

Neoniphon sammara (Spotfin squirrelfish) (Holocentrus sammara)

Taxonomic identifier

47706 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Beryciformes › Holocentridae › Neoniphon

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Rod shaped photoreceptor cells which mediates vision in dim light.
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Rhodopsin		PRO_0000197694
Regions
	Topological domain	1 – 36	36	Extracellular Potential
	Transmembrane	37 – 61	25	Helical; Name=1; Potential
	Topological domain	62 – 73	12	Cytoplasmic Potential
	Transmembrane	74 – 98	25	Helical; Name=2; Potential
	Topological domain	99 – 113	15	Extracellular Potential
	Transmembrane	114 – 133	20	Helical; Name=3; Potential
	Topological domain	134 – 152	19	Cytoplasmic Potential
	Transmembrane	153 – 176	24	Helical; Name=4; Potential
	Topological domain	177 – 202	26	Extracellular Potential
	Transmembrane	203 – 230	28	Helical; Name=5; Potential
	Topological domain	231 – 252	22	Cytoplasmic Potential
	Transmembrane	253 – 276	24	Helical; Name=6; Potential
	Topological domain	277 – 284	8	Extracellular Potential
	Transmembrane	285 – 309	25	Helical; Name=7; Potential
	Topological domain	310 – 351	42	Cytoplasmic Potential
Amino acid modifications
	Modified residue	296	1	N6-(retinylidene)lysine By similarity
	Lipidation	323	1	S-palmitoyl cysteine By similarity
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Disulfide bond	110 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P79812 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 6EF83053459E2C93	FASTA	351	39,369
	10 20 30 40 50 60 MNGTEGPYFY VPMVNTTGVV RSPYEYPQYY LVNPAAFAVL GAYMFFLIIF GFPINFLTLY 70 80 90 100 110 120 VTLEHKKLRT PLNYILLNLA VADLFMVIGG FTTTMYSSMH GYFVLGRLGC NLEGFSATLG 130 140 150 160 170 180 GMISLWSLAV LAIERWVVVC KPTSNFRFGE NHAIMGVSLT WTMALACTVP PLVGWSRYIP 190 200 210 220 230 240 EGMQCSCGID YYTRAEGFNN ESFVLYMFFC HFMVPLIIIF FCYGRLLCAV KEAAAAQQES 250 260 270 280 290 300 ETTQRAEREV TRMVILMVIG YLVCWLPYAS VAWFIFTHQG SEFGPLFMTI PAFFAKSSSI 310 320 330 340 350 YNPVIYICMN KQFRNCMITT LFCGKNPFEG EEEGASSTKT EASSASSVSP A

References

[1]	"Molecular phylogeny of 11 holocentrid fishes (Order Beryciformes) inferred from rhodopsin cDNA and cytochrome b." Toller W.W., Moses K., McFall-Ngai M.J. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57536 mRNA. Translation: AAB39524.1.
3D structure databases
ProteinModelPortal	P79812.
SMR	P79812. Positions 1-351.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG107442.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001760. Opsin. IPR000732. Rhodopsin. IPR019477. Rhodopsin_N. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF10413. Rhodopsin_N. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00579. RHODOPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPSD_NEOSA

Accession

Primary (citable) accession number: P79812

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 30, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program