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Protein

Serotonin N-acetyltransferase

Gene

AANAT

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the N-acetylation of serotonin into N-acetylserotonin.By similarity

Catalytic activityi

Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine.By similarity

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetylserotonin O-methyltransferase (ASMT), Serotonin N-acetyltransferase (AANAT)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Important for the catalytic mechanism; involved in substrate deprotonationBy similarity1
Sitei120Important for the catalytic mechanism; involved in substrate deprotonationBy similarity1
Binding sitei122Substrate; via amide nitrogenBy similarity1
Binding sitei157Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to cAMP Source: Ensembl
  • cellular response to UV-A Source: AgBase
  • circadian rhythm Source: GO_Central
  • melatonin biosynthetic process Source: AgBase
  • N-terminal protein amino acid acetylation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biological rhythms, Melatonin biosynthesis

Enzyme and pathway databases

BRENDAi2.3.1.87. 1306.
ReactomeiR-GGA-209931. Serotonin and melatonin biosynthesis.
UniPathwayiUPA00837; UER00815.

Names & Taxonomyi

Protein namesi
Recommended name:
Serotonin N-acetyltransferase (EC:2.3.1.87By similarity)
Short name:
Serotonin acetylase
Alternative name(s):
Aralkylamine N-acetyltransferase
Short name:
AA-NAT
Gene namesi
Name:AANAT
Synonyms:SNAT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 18

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745871 – 205Serotonin N-acetyltransferaseAdd BLAST205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Phosphothreonine; by PKABy similarity1
Modified residuei203PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated; cAMP-dependent phosphorylation on both N-terminal and C-terminal sites regulates AANAT activity through allowing interaction with 14-3-3 proteins and protecting the enzyme against proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP79774.

Expressioni

Tissue specificityi

Expressed in follicular pineal cells and retinal photoreceptors with five times more expression in the pineal gland.1 Publication

Inductioni

Exhibits circadian rhythm pattern in the pineal gland with highest levels at ZT 24. Expression in the retinal photoreceptor cells oscillates in a circadian manner.2 Publications

Gene expression databases

BgeeiENSGALG00000001955.

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with YWHAZ; the interaction requires phosphorylation on Thr-29, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-203 site, the other ywhaz monomer with similar effect (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000003020.

Structurei

3D structure databases

ProteinModelPortaliP79774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 194N-acetyltransferasePROSITE-ProRule annotationAdd BLAST162

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 124Acetyl-CoA bindingBy similarity3
Regioni130 – 135Acetyl-CoA bindingBy similarity6
Regioni166 – 168Acetyl-CoA bindingBy similarity3

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4144. Eukaryota.
COG0454. LUCA.
GeneTreeiENSGT00390000015579.
HOGENOMiHOG000115812.
HOVERGENiHBG016332.
InParanoidiP79774.
KOiK00669.
OMAiRGHAFMR.
OrthoDBiEOG091G0KJP.
PhylomeDBiP79774.
TreeFamiTF331622.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P79774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVLGAVPFL KPTPLQGPRN SPGRQRRHTL PASEFRCLSP EDAVSVFEIE
60 70 80 90 100
REAFISVSGD CPLHLDEIRH FLTLCPELSL GWFEEGRLVA FIIGSLWDQD
110 120 130 140 150
RLSQAALTLH NPRGTAVHIH VLAVHRTFRQ QGKGSILMWR YLQYLRCLPC
160 170 180 190 200
ARRAVLMCED FLVPFYEKCG FVAVGPCQVT VGTLAFTEMQ HEVRGHAFMR

RNSGC
Length:205
Mass (Da):23,187
Last modified:May 1, 1997 - v1
Checksum:i40871147793A4D80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46502 mRNA. Translation: AAB40942.1.
RefSeqiNP_990489.1. NM_205158.1.
XP_015150605.1. XM_015295119.1.
UniGeneiGga.634.

Genome annotation databases

EnsembliENSGALT00000003024; ENSGALP00000003020; ENSGALG00000001955.
GeneIDi396066.
KEGGigga:396066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46502 mRNA. Translation: AAB40942.1.
RefSeqiNP_990489.1. NM_205158.1.
XP_015150605.1. XM_015295119.1.
UniGeneiGga.634.

3D structure databases

ProteinModelPortaliP79774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000003020.

Proteomic databases

PaxDbiP79774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000003024; ENSGALP00000003020; ENSGALG00000001955.
GeneIDi396066.
KEGGigga:396066.

Organism-specific databases

CTDi15.

Phylogenomic databases

eggNOGiKOG4144. Eukaryota.
COG0454. LUCA.
GeneTreeiENSGT00390000015579.
HOGENOMiHOG000115812.
HOVERGENiHBG016332.
InParanoidiP79774.
KOiK00669.
OMAiRGHAFMR.
OrthoDBiEOG091G0KJP.
PhylomeDBiP79774.
TreeFamiTF331622.

Enzyme and pathway databases

UniPathwayiUPA00837; UER00815.
BRENDAi2.3.1.87. 1306.
ReactomeiR-GGA-209931. Serotonin and melatonin biosynthesis.

Miscellaneous databases

PROiP79774.

Gene expression databases

BgeeiENSGALG00000001955.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNAT_CHICK
AccessioniPrimary (citable) accession number: P79774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.