P79756 (OPSD_GAMAF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhodopsin

Gene names

Name:

rho

Organism

Gambusia affinis (Western mosquitofish)

Taxonomic identifier

33528 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Smegmamorpha › Atherinomorpha › Cyprinodontiformes › Cyprinodontoidei › Poeciliidae › Poeciliinae › Gambusia

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Rod shaped photoreceptor cells which mediates vision in dim light.
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological_process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Rhodopsin		PRO_0000197674
Regions
	Topological domain	1 – 36	36	Extracellular Potential
	Transmembrane	37 – 61	25	Helical; Name=1; Potential
	Topological domain	62 – 73	12	Cytoplasmic Potential
	Transmembrane	74 – 98	25	Helical; Name=2; Potential
	Topological domain	99 – 113	15	Extracellular Potential
	Transmembrane	114 – 133	20	Helical; Name=3; Potential
	Topological domain	134 – 152	19	Cytoplasmic Potential
	Transmembrane	153 – 176	24	Helical; Name=4; Potential
	Topological domain	177 – 202	26	Extracellular Potential
	Transmembrane	203 – 230	28	Helical; Name=5; Potential
	Topological domain	231 – 252	22	Cytoplasmic Potential
	Transmembrane	253 – 276	24	Helical; Name=6; Potential
	Topological domain	277 – 284	8	Extracellular Potential
	Transmembrane	285 – 309	25	Helical; Name=7; Potential
	Topological domain	310 – 354	45	Cytoplasmic Potential
Amino acid modifications
	Modified residue	296	1	N6-(retinylidene)lysine By similarity
	Lipidation	322	1	S-palmitoyl cysteine By similarity
	Lipidation	323	1	S-palmitoyl cysteine By similarity
	Glycosylation	2	1	N-linked (GlcNAc...) By similarity
	Glycosylation	15	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	110 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P79756 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 1CE46D74A03D406B	FASTA	354	39,644
	10 20 30 40 50 60 MNGTEGPYFY VPMVNTTGIV RSPYEYPQYY LVSPAAYACL GAYMFFLILV GFPVNFLTLY 70 80 90 100 110 120 VTIEHKKLRT PLNYILLNLA VADLFMVFGG FTTTIYTSMH GYFVLGRLGC NLEGYFATLG 130 140 150 160 170 180 GEIGLWSLVV LAVERWLVVC KPISNFRFTE NHAIMGLVFT WIMANACAAP PLLGWSRYIP 190 200 210 220 230 240 EGMQCSCGVD YYTRAEGFNN ESFVIYMFIC HFCIPLVVVF FCYGRLLCAV KEAAAAQQES 250 260 270 280 290 300 ETTQRAEREV TRMVVILVIG FLVCWTPYAS VAWYIFSNQG SEFGPLFMTI PAFFAKSSSI 310 320 330 340 350 YNPMIYICMN KQFRHCMITT LCCGKNPFEE EEGASTTASK TEASSVSSSS VSPA

References

[1]	"Opsin sequences of the rod visual pigments in two species of Poeciliid fish." Archer S.N., Hirano J. J. Fish Biol. 51:215-219(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holbrooki. Tissue: Retina.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11146 mRNA. Translation: CAA72033.1.
3D structure databases
ProteinModelPortal	P79756.
SMR	P79756. Positions 1-349.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG107442.
Family and domain databases
Gene3D	4.10.840.10. 1 hit.
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. IPR000732. Rhodopsin. IPR019477. Rhodopsin_N. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF10413. Rhodopsin_N. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00579. RHODOPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OPSD_GAMAF

Accession

Primary (citable) accession number: P79756

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program