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Protein

Rhodopsin

Gene

rho

Organism
Gambusia affinis (Western mosquitofish) (Heterandria affinis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. photoreceptor activity Source: UniProtKB-KW

GO - Biological processi

  1. phototransduction Source: UniProtKB-KW
  2. protein-chromophore linkage Source: UniProtKB-KW
  3. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Chromophore

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin
Gene namesi
Name:rho
OrganismiGambusia affinis (Western mosquitofish) (Heterandria affinis)
Taxonomic identifieri33528 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeCyprinodontiformesPoeciliidaePoeciliinaeGambusia

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3636ExtracellularSequence AnalysisAdd
BLAST
Transmembranei37 – 6125Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini62 – 7312CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei74 – 9825Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini99 – 11315ExtracellularSequence AnalysisAdd
BLAST
Transmembranei114 – 13320Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini134 – 15219CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei153 – 17624Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini177 – 20226ExtracellularSequence AnalysisAdd
BLAST
Transmembranei203 – 23028Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini231 – 25222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei253 – 27624Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini277 – 2848ExtracellularSequence Analysis
Transmembranei285 – 30925Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini310 – 35445CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354RhodopsinPRO_0000197674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)By similarity
Glycosylationi15 – 151N-linked (GlcNAc...)By similarity
Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
Modified residuei296 – 2961N6-(retinylidene)lysineBy similarity
Lipidationi322 – 3221S-palmitoyl cysteineBy similarity
Lipidationi323 – 3231S-palmitoyl cysteineBy similarity

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Expressioni

Tissue specificityi

Rod shaped photoreceptor cells which mediates vision in dim light.

Structurei

3D structure databases

ProteinModelPortaliP79756.
SMRiP79756. Positions 1-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG107442.

Family and domain databases

Gene3Di4.10.840.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
IPR000732. Rhodopsin.
IPR019477. Rhodopsin_N.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF10413. Rhodopsin_N. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00579. RHODOPSIN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P79756-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGTEGPYFY VPMVNTTGIV RSPYEYPQYY LVSPAAYACL GAYMFFLILV
60 70 80 90 100
GFPVNFLTLY VTIEHKKLRT PLNYILLNLA VADLFMVFGG FTTTIYTSMH
110 120 130 140 150
GYFVLGRLGC NLEGYFATLG GEIGLWSLVV LAVERWLVVC KPISNFRFTE
160 170 180 190 200
NHAIMGLVFT WIMANACAAP PLLGWSRYIP EGMQCSCGVD YYTRAEGFNN
210 220 230 240 250
ESFVIYMFIC HFCIPLVVVF FCYGRLLCAV KEAAAAQQES ETTQRAEREV
260 270 280 290 300
TRMVVILVIG FLVCWTPYAS VAWYIFSNQG SEFGPLFMTI PAFFAKSSSI
310 320 330 340 350
YNPMIYICMN KQFRHCMITT LCCGKNPFEE EEGASTTASK TEASSVSSSS

VSPA
Length:354
Mass (Da):39,644
Last modified:May 1, 1997 - v1
Checksum:i1CE46D74A03D406B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11146 mRNA. Translation: CAA72033.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11146 mRNA. Translation: CAA72033.1.

3D structure databases

ProteinModelPortaliP79756.
SMRiP79756. Positions 1-349.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG107442.

Family and domain databases

Gene3Di4.10.840.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
IPR000732. Rhodopsin.
IPR019477. Rhodopsin_N.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF10413. Rhodopsin_N. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00579. RHODOPSIN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Opsin sequences of the rod visual pigments in two species of Poeciliid fish."
    Archer S.N., Hirano J.
    J. Fish Biol. 51:215-219(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Holbrooki.
    Tissue: Retina.

Entry informationi

Entry nameiOPSD_GAMAF
AccessioniPrimary (citable) accession number: P79756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: January 7, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.