P79745 (P3F3B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain, class 3, transcription factor 3-B

Alternative name(s):
Brain-specific homeobox/POU domain protein 1.0
Short name=Brain-1.0
Short name=zfBrn-1.0
POU domain protein 1
Short name=ZFPOU1
POU domain protein 23
Short name=ZP-23

Gene names

Name:	pou3f3b
Synonyms:	brn-1.0, pou1, pou23, zp23, zp23pou

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that may play important roles in patterning the embryonic brain.
Subcellular location	Nucleus.
Tissue specificity	Predominantly expressed in the central nervous system. Ref.1 Ref.2 Ref.4
Developmental stage	First expressed at early neurula stage (9-12 hours). Expression then increases greatly at the 16-somite stage, reached the maximum level at 36 hr of development, and thereafter decreased. Only expressed in adults in the brain. Ref.1 Ref.2 Ref.4
Sequence similarities	Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P79745-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P79745-2)

The sequence of this isoform differs from the canonical sequence as follows:
     407-443: GHFLVDYLKDASLTGPSEPGDQRVTTTSSFHQVILAH → VSADTPPPSMDCKRMFSET

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	POU domain, class 3, transcription factor 3-B		PRO_0000100771
Regions
	Domain	238 – 312	75	POU-specific
	DNA binding	330 – 389	60	Homeobox
	Compositional bias	84 – 98	15	Ala-rich
	Compositional bias	165 – 169	5	Poly-Gln
	Compositional bias	208 – 235	28	His-rich
Amino acid modifications
	Modified residue	317	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	407 – 443	37	GHFLV…VILAH → VSADTPPPSMDCKRMFSET in isoform Short.		VSP_002341
Experimental info
	Sequence conflict	109	1	M → I in BAA02377. Ref.1
	Sequence conflict	116	1	Q → QQ in AAH56549. Ref.3
	Sequence conflict	116	1	Q → QQ in AAI63489. Ref.3
	Sequence conflict	135	1	H → N in BAA02377. Ref.1
	Sequence conflict	146	1	H → Y in BAA02377. Ref.1
	Sequence conflict	170	1	S → F in BAA02377. Ref.1
	Sequence conflict	174 – 175	2	SQ → FA in BAA02377. Ref.1
	Sequence conflict	185	1	L → H in BAA02377. Ref.1
	Sequence conflict	263	1	T → K in BAA02377. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 5F2A6DC6BB333F5C	FASTA	443	47,453
	10 20 30 40 50 60 MATAASNPYL ASSSILSSGS IVHSDSGGGM QQGSAAVTSV SGGYRGDPTV KMVQSDFMQG 70 80 90 100 110 120 AMAASNGGHM LSHAHQWVTS LPHAAAAAAA AAVAAAEAGS PWSSSPVGMT GSPQQQDVKN 130 140 150 160 170 180 NSGRDDLHSG TALHHRAPHL GPHQTHAGAW GSTTAAHIPS LTGSQQQQQS LIYSQPGGFT 190 200 210 220 230 240 VNGMLSPPGS QSLVHPGLVR GDTPELDHSS HHHHHHHQHQ HHQQAHHGVN SHDPHSDEDT 250 260 270 280 290 300 PTSDDLEHFA KQFKQRRIKL GFTQADVGLA LGTLYGNVFS QTTICRFEAL QLSFKNMCKL 310 320 330 340 350 360 KPLLNKWLEE ADSSTGSPTS IDKIAAQGRK RKKRTSIEVS VKGALESHFL KCPKPSAQEI 370 380 390 400 410 420 TSLADNLQLE KEVVRVWFCN RRQKEKRMTP PGVPQTPEDV YSQVGNGHFL VDYLKDASLT 430 440 GPSEPGDQRV TTTSSFHQVI LAH
	Isoform Short [UniParc]. Checksum: BAAC6D655310F131	FASTA	425	45,527
	10 20 30 40 50 60 MATAASNPYL ASSSILSSGS IVHSDSGGGM QQGSAAVTSV SGGYRGDPTV KMVQSDFMQG 70 80 90 100 110 120 AMAASNGGHM LSHAHQWVTS LPHAAAAAAA AAVAAAEAGS PWSSSPVGMT GSPQQQDVKN 130 140 150 160 170 180 NSGRDDLHSG TALHHRAPHL GPHQTHAGAW GSTTAAHIPS LTGSQQQQQS LIYSQPGGFT 190 200 210 220 230 240 VNGMLSPPGS QSLVHPGLVR GDTPELDHSS HHHHHHHQHQ HHQQAHHGVN SHDPHSDEDT 250 260 270 280 290 300 PTSDDLEHFA KQFKQRRIKL GFTQADVGLA LGTLYGNVFS QTTICRFEAL QLSFKNMCKL 310 320 330 340 350 360 KPLLNKWLEE ADSSTGSPTS IDKIAAQGRK RKKRTSIEVS VKGALESHFL KCPKPSAQEI 370 380 390 400 410 420 TSLADNLQLE KEVVRVWFCN RRQKEKRMTP PGVPQTPEDV YSQVGNVSAD TPPPSMDCKR MFSET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A POU-domain gene of zebrafish, ZFPOU1, specifically expressed in the developing neural tissues." Matsuzaki T., Amanuma H., Takeda H. Biochem. Biophys. Res. Commun. 187:1446-1453(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Neurula.
[2]	"Class III POU genes of zebrafish are predominantly expressed in the central nervous system." Spaniol P., Bornmann C., Hauptmann G., Gerster T. Nucleic Acids Res. 24:4874-4881(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Neurula.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT). Tissue: Embryo and Kidney.
[4]	"Developmental expression of class III and IV POU domain genes in the zebrafish." Sampath K., Stuart G.W. Biochem. Biophys. Res. Commun. 219:565-571(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 262-374 (ISOFORM SHORT), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Pharyngula.
[5]	"Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo." Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R. J. Proteome Res. 7:1555-1564(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13045 mRNA. Translation: BAA02377.1. Y07907 mRNA. Translation: CAA69215.1. Y07907 mRNA. Translation: CAA69214.1. BC056320 mRNA. Translation: AAH56320.1. BC056549 mRNA. Translation: AAH56549.1. BC163489 mRNA. Translation: AAI63489.1.
IPI	IPI00506827. IPI00934210.
PIR	JH0710.
RefSeq	NP_571177.2. NM_131102.2. NP_958855.1. NM_201447.1.
UniGene	Dr.29717.
3D structure databases
ProteinModelPortal	P79745.
SMR	P79745. Positions 244-390.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000065952.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000149202; ENSDARP00000123893; ENSDARG00000095896. ENSDART00000149949; ENSDARP00000124688; ENSDARG00000095896.
GeneID	30321.
KEGG	dre:30321.
Organism-specific databases
CTD	30321.
ZFIN	ZDB-GENE-980526-140. pou3f3b.
Phylogenomic databases
eggNOG	NOG261729.
GeneTree	ENSGT00700000104117.
HOGENOM	HOG000116303.
HOVERGEN	HBG053120.
InParanoid	Q6PHH6.
KO	K09365.
OrthoDB	EOG4HDSTS.
Gene expression databases
Bgee	P79745.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. IPR013847. POU. IPR000327. POU_specific. IPR016362. Transcription_factor_POU. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PIRSF	PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806757.

Entry information

Entry name

P3F3B_DANRE

Accession

Primary (citable) accession number: P79745
Secondary accession number(s): B3DJH8 , P31366, P79744, Q6PHH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program