pou3f3b

Functionⁱ

Transcription factor that may play important roles in patterning the embryonic brain.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	330 – 389	HomeoboxPROSITE-ProRule annotationAdd BLAST		60

GO - Molecular functionⁱ

DNA binding transcription factor activity Source: InterPro
sequence-specific DNA binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

brain development Source: InterPro
regulation of transcription by RNA polymerase II Source: InterPro
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, DNA-binding
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: POU domain, class 3, transcription factor 3-B Alternative name(s): Brain-specific homeobox/POU domain protein 1.0 Short name: Brain-1.0 Short name: zfBrn-1.0 POU domain protein 1 Short name: ZFPOU1 POU domain protein 23 Short name: ZP-23
Gene namesⁱ	Name:pou3f3b Synonyms:brn-1.0, pou1, pou23, zp23, zp23pou
Organismⁱ	Danio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifierⁱ	7955 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Osteoglossocephalai › Clupeocephala › Otomorpha › Ostariophysi › Otophysi › Cypriniphysae › Cypriniformes › Cyprinoidea › Cyprinidae › Danio
Proteomesⁱ	UP000000437 Componentⁱ: Chromosome 6

Organism-specific databases

ZFINⁱ	ZDB-GENE-980526-140. pou3f3b.

ZFINⁱ

ZDB-GENE-980526-140. pou3f3b.

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000100771	1 – 443	POU domain, class 3, transcription factor 3-BAdd BLAST		443

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	317	Phosphoserine1 Publication		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P79745.
PRIDEⁱ	P79745.

PTM databases

iPTMnetⁱ	P79745.

iPTMnetⁱ

P79745.

Expressionⁱ

Tissue specificityⁱ

Predominantly expressed in the central nervous system.3 Publications

Developmental stageⁱ

First expressed at early neurula stage (9-12 hours). Expression then increases greatly at the 16-somite stage, reached the maximum level at 36 hr of development, and thereafter decreased. Only expressed in adults in the brain.3 Publications

Gene expression databases

Bgeeⁱ	ENSDARG00000095896.

Bgeeⁱ

ENSDARG00000095896.

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	7955.ENSDARP00000124688.

STRINGⁱ

7955.ENSDARP00000124688.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P79745.
SMRⁱ	P79745.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	238 – 312	POU-specificPROSITE-ProRule annotationAdd BLAST		75

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	84 – 98	Ala-richAdd BLAST	15
Compositional biasⁱ	165 – 169	Poly-Gln	5
Compositional biasⁱ	208 – 235	His-richAdd BLAST	28

Sequence similaritiesⁱ

Belongs to the POU transcription factor family. Class-3 subfamily.Curated

Keywords - Domainⁱ

Homeobox

Phylogenomic databases

eggNOGⁱ	KOG3802. Eukaryota. ENOG410XQ7X. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118935.
HOGENOMⁱ	HOG000116303.
HOVERGENⁱ	HBG053120.
InParanoidⁱ	P79745.
KEGG Orthology (KO) More...KOⁱ	K09365.
OMAⁱ	PHQGHPG.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0U0S.
PhylomeDBⁱ	P79745.
TreeFamⁱ	TF316413.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00086. homeodomain. 1 hit.
InterProⁱ	View protein in InterPro IPR009057. Homeobox-like_sf. IPR017970. Homeobox_CS. IPR001356. Homeobox_dom. IPR010982. Lambda_DNA-bd_dom_sf. IPR013847. POU. IPR000327. POU_dom. IPR016362. TF_POU_3.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit.
PIRSFⁱ	PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSⁱ	PR00028. POUDOMAIN.
SMARTⁱ	View protein in SMART SM00389. HOX. 1 hit. SM00352. POU. 1 hit.
SUPFAMⁱ	SSF46689. SSF46689. 1 hit. SSF47413. SSF47413. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit.

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform Long (identifier: P79745-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAASNPYL ASSSILSSGS IVHSDSGGGM QQGSAAVTSV SGGYRGDPTV 
        60         70         80         90        100
KMVQSDFMQG AMAASNGGHM LSHAHQWVTS LPHAAAAAAA AAVAAAEAGS 
       110        120        130        140        150
PWSSSPVGMT GSPQQQDVKN NSGRDDLHSG TALHHRAPHL GPHQTHAGAW 
       160        170        180        190        200
GSTTAAHIPS LTGSQQQQQS LIYSQPGGFT VNGMLSPPGS QSLVHPGLVR 
       210        220        230        240        250
GDTPELDHSS HHHHHHHQHQ HHQQAHHGVN SHDPHSDEDT PTSDDLEHFA 
       260        270        280        290        300
KQFKQRRIKL GFTQADVGLA LGTLYGNVFS QTTICRFEAL QLSFKNMCKL 
       310        320        330        340        350
KPLLNKWLEE ADSSTGSPTS IDKIAAQGRK RKKRTSIEVS VKGALESHFL 
       360        370        380        390        400
KCPKPSAQEI TSLADNLQLE KEVVRVWFCN RRQKEKRMTP PGVPQTPEDV 
       410        420        430        440 
YSQVGNGHFL VDYLKDASLT GPSEPGDQRV TTTSSFHQVI LAH

Length:443

Mass (Da):47,453

Last modified:May 1, 1997 - v1

Checksum:ⁱ5F2A6DC6BB333F5C

GO

Isoform Short (identifier: P79745-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
407-443: GHFLVDYLKDASLTGPSEPGDQRVTTTSSFHQVILAH → VSADTPPPSMDCKRMFSET

« Hide

        10         20         30         40         50
MATAASNPYL ASSSILSSGS IVHSDSGGGM QQGSAAVTSV SGGYRGDPTV 
        60         70         80         90        100
KMVQSDFMQG AMAASNGGHM LSHAHQWVTS LPHAAAAAAA AAVAAAEAGS 
       110        120        130        140        150
PWSSSPVGMT GSPQQQDVKN NSGRDDLHSG TALHHRAPHL GPHQTHAGAW 
       160        170        180        190        200
GSTTAAHIPS LTGSQQQQQS LIYSQPGGFT VNGMLSPPGS QSLVHPGLVR 
       210        220        230        240        250
GDTPELDHSS HHHHHHHQHQ HHQQAHHGVN SHDPHSDEDT PTSDDLEHFA 
       260        270        280        290        300
KQFKQRRIKL GFTQADVGLA LGTLYGNVFS QTTICRFEAL QLSFKNMCKL 
       310        320        330        340        350
KPLLNKWLEE ADSSTGSPTS IDKIAAQGRK RKKRTSIEVS VKGALESHFL 
       360        370        380        390        400
KCPKPSAQEI TSLADNLQLE KEVVRVWFCN RRQKEKRMTP PGVPQTPEDV 
       410        420 
YSQVGNVSAD TPPPSMDCKR MFSET

Show »

Length:425

Mass (Da):45,527

Checksum:ⁱBAAC6D655310F131

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	109	M → I in BAA02377 (PubMed:1417821).Curated	1
Sequence conflictⁱ	116	Q → QQ in AAH56549 (Ref. 3) Curated	1
Sequence conflictⁱ	116	Q → QQ in AAI63489 (Ref. 3) Curated	1
Sequence conflictⁱ	135	H → N in BAA02377 (PubMed:1417821).Curated	1
Sequence conflictⁱ	146	H → Y in BAA02377 (PubMed:1417821).Curated	1
Sequence conflictⁱ	170	S → F in BAA02377 (PubMed:1417821).Curated	1
Sequence conflictⁱ	174 – 175	SQ → FA in BAA02377 (PubMed:1417821).Curated	2
Sequence conflictⁱ	185	L → H in BAA02377 (PubMed:1417821).Curated	1
Sequence conflictⁱ	263	T → K in BAA02377 (PubMed:1417821).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_002341	407 – 443	GHFLV…VILAH → VSADTPPPSMDCKRMFSET in isoform Short. 4 PublicationsAdd BLAST		37

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D13045 mRNA. Translation: BAA02377.1. Y07907 mRNA. Translation: CAA69215.1. Y07907 mRNA. Translation: CAA69214.1. BC056320 mRNA. Translation: AAH56320.1. BC056549 mRNA. Translation: AAH56549.1. BC163489 mRNA. Translation: AAI63489.1.
PIRⁱ	JH0710.
NCBI Reference Sequences More...RefSeqⁱ	NP_571177.2. NM_131102.3. [P79745-2] NP_958855.2. NM_201447.2. [P79745-1]
UniGeneⁱ	Dr.29717.

Genome annotation databases

Ensemblⁱ	ENSDART00000149202; ENSDARP00000123893; ENSDARG00000095896. [P79745-2] ENSDART00000149949; ENSDARP00000124688; ENSDARG00000095896. [P79745-1]
GeneIDⁱ	30321.
KEGGⁱ	dre:30321.

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P79745	A0A147A187 A0A146QS68 UPI00097338DB UPI000CEB041E UPI0009A02506 UPI000B4EF354 UPI000CEACE48 UPI0008FA29D5 I3K726 UPI0003EC0D18 +42	Fundulus heteroclitus (Killifish) (Mummichog) Esox lucius (Northern pike) Salvelinus alpinus (Arctic char) (Salmo alpinus) Oncorhynchus kisutch (Coho salmon) (Salmo kisutch) Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) Cyprinus carpio (Common carp) Oreochromis niloticus (Nile tilapia) (Tilapia nilotica) Neolamprologus brichardi (Fairy cichlid) (Lamprologus brichardi) Maylandia zebra (zebra mbuna) Hippocampus comes (Tiger tail seahorse) And more	443	UniRef90_P79745	Cluster: POU domain, class 3, transcription factor 3-B	53

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P79745	A0A147A187 A0A146QS68 UPI00097338DB UPI000B4EF354 UPI0009A02506 UPI000CEB041E UPI000CEACE48 UPI0008FA29D5 UPI0003EC0D18 UPI00032A3787 +250	Fundulus heteroclitus (Killifish) (Mummichog) Esox lucius (Northern pike) Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) Oncorhynchus kisutch (Coho salmon) (Salmo kisutch) Salvelinus alpinus (Arctic char) (Salmo alpinus) Cyprinus carpio (Common carp) Neolamprologus brichardi (Fairy cichlid) (Lamprologus brichardi) Maylandia zebra (zebra mbuna) Oreochromis niloticus (Nile tilapia) (Tilapia nilotica) Hippocampus comes (Tiger tail seahorse) And more	443	UniRef50_P79745	Cluster: POU domain, class 3, transcription factor 3-B	261
P79745-2	UPI000CEAC403 UPI000CEB36C6 UPI000577145A UPI000CD61B19 UPI000643F74A UPI000CD64B7D UPI0007B938B7 UPI0007AD6395 UPI0007B7C44C UPI0007AD233E +10	Salvelinus alpinus (Arctic char) (Salmo alpinus) Esox lucius (Northern pike) Paramormyrops kingsleyae Clupea harengus (Atlantic herring) Sinocyclocheilus anshuiensis Sinocyclocheilus grahami (Dianchi golden-line fish) (Barbus grahami) Cyprinus carpio (Common carp) Gasterosteus aculeatus (Three-spined stickleback) Branchiostoma floridae (Florida lancelet) (Amphioxus) Branchiostoma belcheri (Amphioxus) And more	425	UniRef50_P79745-2	Cluster: Isoform Short of POU domain, class 3, transcription factor 3-B	21

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D13045 mRNA. Translation: BAA02377.1. Y07907 mRNA. Translation: CAA69215.1. Y07907 mRNA. Translation: CAA69214.1. BC056320 mRNA. Translation: AAH56320.1. BC056549 mRNA. Translation: AAH56549.1. BC163489 mRNA. Translation: AAI63489.1.
PIRⁱ	JH0710.
NCBI Reference Sequences More...RefSeqⁱ	NP_571177.2. NM_131102.3. [P79745-2] NP_958855.2. NM_201447.2. [P79745-1]
UniGeneⁱ	Dr.29717.

3D structure databases

ProteinModelPortalⁱ	P79745.
SMRⁱ	P79745.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	7955.ENSDARP00000124688.

STRINGⁱ

7955.ENSDARP00000124688.

PTM databases

iPTMnetⁱ	P79745.

iPTMnetⁱ

P79745.

Proteomic databases

PaxDbⁱ	P79745.
PRIDEⁱ	P79745.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSDART00000149202; ENSDARP00000123893; ENSDARG00000095896. [P79745-2] ENSDART00000149949; ENSDARP00000124688; ENSDARG00000095896. [P79745-1]
GeneIDⁱ	30321.
KEGGⁱ	dre:30321.

Organism-specific databases

CTDⁱ	30321.
ZFINⁱ	ZDB-GENE-980526-140. pou3f3b.

Phylogenomic databases

eggNOGⁱ	KOG3802. Eukaryota. ENOG410XQ7X. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118935.
HOGENOMⁱ	HOG000116303.
HOVERGENⁱ	HBG053120.
InParanoidⁱ	P79745.
KEGG Orthology (KO) More...KOⁱ	K09365.
OMAⁱ	PHQGHPG.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0U0S.
PhylomeDBⁱ	P79745.
TreeFamⁱ	TF316413.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P79745.

PROⁱ

PR:P79745.

Gene expression databases

Bgeeⁱ	ENSDARG00000095896.

Bgeeⁱ

ENSDARG00000095896.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00086. homeodomain. 1 hit.
InterProⁱ	View protein in InterPro IPR009057. Homeobox-like_sf. IPR017970. Homeobox_CS. IPR001356. Homeobox_dom. IPR010982. Lambda_DNA-bd_dom_sf. IPR013847. POU. IPR000327. POU_dom. IPR016362. TF_POU_3.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit.
PIRSFⁱ	PIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSⁱ	PR00028. POUDOMAIN.
SMARTⁱ	View protein in SMART SM00389. HOX. 1 hit. SM00352. POU. 1 hit.
SUPFAMⁱ	SSF46689. SSF46689. 1 hit. SSF47413. SSF47413. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	P3F3B_DANRE
Accessionⁱ	P79745Primary (citable) accession number: P79745 Secondary accession number(s): B3DJH8 , P31366, P79744, Q6PHH6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	May 1, 1997
	Last modified:	March 28, 2018
	This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P79745 (P3F3B_DANRE)

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POU domain, class 3, transcription factor 3-B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ