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Protein

GTP-binding nuclear protein Ran

Gene

ran

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis.By similarity

Cofactori

Mg2+By similarityNote: Mg2+ interacts primarily with the phosphate groups of the bound guanine nucleotide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67GTP; via amide nitrogenBy similarity1
Sitei68Essential for GTP hydrolysisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24GTPBy similarity8
Nucleotide bindingi35 – 41GTPBy similarity7
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi149 – 151GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • GTP metabolic process Source: UniProtKB
  • mitotic sister chromatid segregation Source: UniProtKB
  • neural retina development Source: ZFIN
  • protein import into nucleus Source: UniProtKB
  • retina development in camera-type eye Source: ZFIN
  • ribosomal subunit export from nucleus Source: GO_Central
  • snRNA import into nucleus Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processProtein transport, Transport
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
R-DRE-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein Ran
Alternative name(s):
GTPase Ran
Ras-related nuclear protein
Gene namesi
Name:ran
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 14

Organism-specific databases

ZFINiZDB-GENE-990415-88 ran

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002087041 – 215GTP-binding nuclear protein RanAdd BLAST215

Proteomic databases

PaxDbiP79735
PRIDEiP79735

Expressioni

Tissue specificityi

Ubiquitous, with the highest expression in ovary, muscle, eye and brain.1 Publication

Developmental stagei

Expression is low at 2 hpf and starts to increase steadily after 12 hpf to reach adult levels at 72 hpf.1 Publication

Gene expression databases

BgeeiENSDARG00000057026
ExpressionAtlasiP79735 baseline and differential

Interactioni

Subunit structurei

Monomer. Interacts with rangap1, which promotes ran-mediated GTP hydrolysis. Interacts with kpnb1. Interaction with kpnb1 inhibits rangap1-mediated stimulation of GTPase activity. Interacts with rcc1 which promotes the exchange of ran-bound GDP by GTP. Interaction with kpnb1 inhibits rcc1-mediated exchange of ran-bound GDP by GTP. Interacts (GTP-bound form) with tnpo1; the interaction is direct. Interacts with kpnb1 and with tnpo1; both inhibit ran GTPase activity. Interacts (via C-terminus) with ranbp1, which alleviates the inhibition of ran GTPase activity. Interacts with rangrf, which promotes the release of bound guanine nucleotide. Rangrf and rcc1 compete for an overlapping binding site on ran. Identified in a complex with kpna2 and cse1l; interaction with ranbp1 mediates dissociation of ran from this complex. Interaction with both ranbp1 and kpna2 promotes dissociation of the complex between ran and kpnb1. Identified in a complex composed of ran, rangap1 and ranbp1. Identified in a complex that contains tnpo1, ran and ranbp1. Identified in a nuclear export complex with xpo1. Interaction with ranbp1 or ranbp2 induces a conformation change in the complex formed by xpo1 and ran that triggers the release of the nuclear export signal of cargo proteins. Component of a nuclear export receptor complex composed of kpnb1, ran, snupn and xpo1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000108629

Structurei

3D structure databases

ProteinModelPortaliP79735
SMRiP79735
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 215Interaction with RANBP1By similarity6

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

eggNOGiKOG0096 Eukaryota
ENOG410XNRS LUCA
GeneTreeiENSGT00840000129836
HOGENOMiHOG000216664
HOVERGENiHBG107376
InParanoidiP79735
KOiK07936
OMAiYNVWDTA
OrthoDBiEOG091G0L3X
PhylomeDBiP79735
TreeFamiTF106302

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR002041 Ran_GTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PANTHERiPTHR24071 PTHR24071, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
PRINTSiPR00627 GTPRANTC4
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51418 RAN, 1 hit

Sequencei

Sequence statusi: Complete.

P79735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENEPQVQF KLVLVGDGGT GKTTFVKRHL TGEFEKKYVA TLGVEVHPLV
60 70 80 90 100
FHTNRGAIKY NVWDTAGQEK FGGLRDGYYI QAQCAIIMFD VTSRVTYKNV
110 120 130 140 150
PNWHRDLVRV CENIPIVLCG NKVDIKDRKV KAKSIVFHRK KNLQYYDISA
160 170 180 190 200
KSNYNFEKPF LWLARKLIGD PNLEFVEMPA LAPPEIAMDP TLAAQYEHDL
210
KVASETALPD EDDDL
Length:215
Mass (Da):24,460
Last modified:May 1, 1997 - v1
Checksum:i26A49A42440133A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61395 mRNA Translation: AAB97093.1
BC050517 mRNA Translation: AAH50517.2
RefSeqiNP_571384.1, NM_131309.3
XP_005173151.1, XM_005173094.3
UniGeneiDr.32354

Genome annotation databases

EnsembliENSDART00000034438; ENSDARP00000032532; ENSDARG00000057026
ENSDART00000128717; ENSDARP00000108629; ENSDARG00000057026
ENSDART00000144503; ENSDARP00000121831; ENSDARG00000057026
GeneIDi30572
KEGGidre:30572

Similar proteinsi

Entry informationi

Entry nameiRAN_DANRE
AccessioniPrimary (citable) accession number: P79735
Secondary accession number(s): Q7ZU75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health