Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P79728 (EFNA5_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ephrin-A5b
Alternative name(s):
AL-1
EPH-related receptor tyrosine kinase ligand 7
Short name=LERK-7
ZfEPHL4
Gene names
Name:efna5b
Synonyms:al1, efna5, eplg7, lerk7
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the epha3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor epha2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation By similarity. Controls axon growth and may be involved in the creation of the retino-tectal map.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Potential.

Tissue specificity

Widespread expression in the embryo.

Developmental stage

Expressed in the presumptive midbrain of developing embryos from the six-somite stage. By 24 hours, strongly expressed in the midbrain caudal to the presumptive tectum. At later stages, maintained at the posterior margin of the tectum.

Sequence similarities

Belongs to the ephrin family.

Contains 1 ephrin RBD (ephrin receptor-binding) domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Inferred from Biological aspect of Ancestor. Source: RefGenome

ephrin receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

mesoderm migration involved in gastrulation

Inferred from mutant phenotype PubMed 10381569. Source: ZFIN

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of Rac GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of Rho GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of actin cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of focal adhesion assembly

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of microtubule cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

somitogenesis

Inferred from mutant phenotype PubMed 10381569. Source: ZFIN

   Cellular_componentanchored component of external side of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

anchored component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionephrin receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 204184Ephrin-A5b
PRO_0000008385
Propeptide205 – 22824Removed in mature form Potential
PRO_0000008386

Regions

Domain29 – 162134Ephrin RBD

Amino acid modifications

Lipidation2041GPI-anchor amidated serine Potential
Glycosylation371N-linked (GlcNAc...) Potential
Disulfide bond62 ↔ 102 By similarity
Disulfide bond90 ↔ 151 By similarity

Sequences

Sequence LengthMass (Da)Tools
P79728 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 74B3406C05418E6E

FASTA22826,595
        10         20         30         40         50         60 
MLQAEMIVFV GVILWMCVFS QEPSSKVMAD RYAVFWNRTN PRFQRGDYHI DVCINDYLDV 

        70         80         90        100        110        120 
YCPHYEDSVP EERTERYVLY MVNYDGYSTC DHTAKGFKRW ECNRPHSPNG PLKFSEKFQL 

       130        140        150        160        170        180 
FTPFSLGFEF RPGREYYYIS SMITETGRRS CLKLKVFVRP PNGCEKTIGV HDRVFVDDKV 

       190        200        210        220 
DNALEPRDDT SHEAEPSRSD VSTSGLRHQT SRPLLALLLL CISLYLLL 

« Hide

References

[1]"Two Eph receptor tyrosine kinase ligands control axon growth and may be involved in the creation of the retinotectal map in the zebrafish."
Brennan C., Monschau B., Lindberg R., Guthrie B., Drescher U., Bonhoeffer F., Holder N.
Development 124:655-664(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09669 mRNA. Translation: CAA70864.1.
RefSeqNP_571101.1. NM_131026.2.
UniGeneDr.19377.

3D structure databases

ProteinModelPortalP79728.
SMRP79728. Positions 28-164.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID30223.
KEGGdre:30223.

Organism-specific databases

CTD30223.
ZFINZDB-GENE-980526-186. efna5b.

Phylogenomic databases

HOGENOMHOG000234373.
HOVERGENHBG051447.
KOK05462.
PhylomeDBP79728.

Gene expression databases

BgeeP79728.

Family and domain databases

Gene3D2.60.40.420. 1 hit.
InterProIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
PANTHERPTHR11304. PTHR11304. 1 hit.
PfamPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSPR01347. EPHRIN.
ProDomPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF49503. SSF49503. 1 hit.
PROSITEPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806682.
PROP79728.

Entry information

Entry nameEFNA5_DANRE
AccessionPrimary (citable) accession number: P79728
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families