P79690 (CP191_CARAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 19 type 1
EC=1.14.14.1

Alternative name(s):
Brain aromatase
CYPXIXA1
Cytochrome P-450AROM
Estrogen synthase

Gene names

Name:	cyp19a1
Synonyms:	cyp19

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Carassius

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Tissue specificity	Brain specific.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Cytochrome P450 19 type 1		PRO_0000051968
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	14	1	A → T in BAA23757. Ref.2
	Sequence conflict	495	1	A → S in BAA23757. Ref.2
	Sequence conflict	509	1	E → Q in BAA23757. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P79690 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FAAE56AACF3AC646	FASTA	510	58,049
	10 20 30 40 50 60 MEEVLKGTVN FAAAVQVTLM ALTGTLLLIL LHRIFTAKNW RNQSGVPGPG WLLGLGPIMS 70 80 90 100 110 120 YSRFLWMGIG SACNYYNEKY GSIARVWISG EETFILSKSS AVYHVLKSNN YTGRFASKKG 130 140 150 160 170 180 LQCIGMFEQG IIFNSNMALW KKVRTYFTKA LTGPGLQKSV DVCVSATNKQ LNVLQEFTDH 190 200 210 220 230 240 SGHVDVLNLL RCIVVDVSNR LFLRIPLNEK DLLIKIHRYF STWQAVLIQP DVFFRLNFVY 250 260 270 280 290 300 KKYHLAAKEL QDEMGKLVEQ KRQAINNMEK LDETDFATEL IFAQNHDELS VDDVRQCVLE 310 320 330 340 350 360 MVIAAPDTLS ISLFFMLLLL KQNSVVEEQI VQEIQSQIGE RDVESADLQK LNVLERFIKE 370 380 390 400 410 420 SLRFHPVVDF IMRRALEDDE IDGYRVAKGT NLILNIGRMH KSEFFQKPNE FNLENFENTV 430 440 450 460 470 480 PSRYFQPFGC GPRACVGKHI AMVMTKAILV TLLSRFTVCP RHGCTVSTIK QTNNLSMQPV 490 500 510 EEDPDSLAMR FIPRAQNICG DPHLGEKTEE

References

[1]	"Isolation of a goldfish brain cytochrome P450 aromatase cDNA: mRNA expression during the seasonal cycle and after steroid treatment." Gelinas D.M., Pitoc G.A., Callard G.V. Mol. Cell. Endocrinol. 138:81-93(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Two differing cDNAs encoding cytochrome P450arom (aromatase) exist in goldfish." Sudrajat A., Yoshiura Y., Gen K., Suetake H., Aida K. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18974 mRNA. Translation: AAB39408.1. AB009335 mRNA. Translation: BAA23757.1.
3D structure databases
ProteinModelPortal	P79690.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050750.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP191_CARAU

Accession

Primary (citable) accession number: P79690
Secondary accession number(s): O57315

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program