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Protein

MHC class II transactivator

Gene

Ciita

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription, the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: phosphorylates the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi445 – 4528GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MHC class II transactivator (EC:2.3.1.-, EC:2.7.11.1)
Short name:
CIITA
Gene namesi
Name:Ciita
Synonyms:C2ta, Mhc2ta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:108445. Ciita.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11551155MHC class II transactivatorPRO_0000089242Add
BLAST

Post-translational modificationi

Autophosphorylated, affecting interaction with TAF7.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP79621.
PaxDbiP79621.
PRIDEiP79621.

PTM databases

PhosphoSiteiP79621.

Expressioni

Tissue specificityi

Isoform 1 is expressed at very high levels in dendritic cells, at very low levels in spleen and thymus and is not detected in other tissues. Isoform 2 is detected at high levels in spleen and tonsil as well as in a number of B-lymphocyte cell lines, and at very low levels in dendritic cells.1 Publication

Inductioni

By interferon gamma.1 Publication

Gene expression databases

BgeeiP79621.
CleanExiMM_CIITA.
ExpressionAtlasiP79621. baseline and differential.
GenevisibleiP79621. MM.

Interactioni

Subunit structurei

Interacts with ZXDB and ZXDC. Interacts with PML.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198417. 9 interactions.
STRINGi10090.ENSMUSP00000023147.

Structurei

3D structure databases

ProteinModelPortaliP79621.
SMRiP79621. Positions 436-461, 973-1151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini439 – 749311NACHTPROSITE-ProRule annotationAdd
BLAST
Repeati1010 – 103324LRR 1Add
BLAST
Repeati1041 – 106222LRR 2Add
BLAST
Repeati1070 – 109122LRR 3Add
BLAST
Repeati1098 – 111922LRR 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 21040Required for acetyltransferase activityBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi129 – 21587Asp/Glu-rich (acidic)Add
BLAST

Domaini

The acetyltransferase domain is necessary for activation of both class I and class II transcription.By similarity
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.By similarity

Sequence similaritiesi

Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 NACHT domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4308. Eukaryota.
ENOG410ZBX3. LUCA.
HOGENOMiHOG000107439.
HOVERGENiHBG000174.
InParanoidiP79621.
KOiK08060.
OMAiQKRPFPE.
OrthoDBiEOG7P5T07.
PhylomeDBiP79621.
TreeFamiTF352118.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR008095. MHC_II_transact.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 3 hits.
[Graphical view]
PRINTSiPR01719. MHCIIACTVATR.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P79621-1) [UniParc]FASTAAdd to basket

Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNHFQAILAQ VQTLLSSQKP RQVRALLDGL LEEELLSREY HCALLHEPDG
60 70 80 90 100
DALARKISLT LLEKGDLDLT FLSWVCNSLQ APTVERGTSY RDHGDHSLCA
110 120 130 140 150
TMDLGSPEGS YLELLNSDAD PLHLYHLYDQ MDLAGEEEIE LSSEPDTDTI
160 170 180 190 200
NCDQFSKLLQ DMELDEETRE AYANIAELDQ YVFQDTQLEG LSKDLFIEHI
210 220 230 240 250
GAEEGFGENI EIPVEAGQKP QKRRFPEEHA MDSKHRKLVP TSRTSLNYLD
260 270 280 290 300
LPTGHIQIFT TLPQGLWQIS GAGTGLSSVL IYHGEMPQVN QVLPSSSLSI
310 320 330 340 350
PSLPESPDRP GSTSPFTPSA ADLPSMPEPA LTSRVNETED TSPSPCQEGP
360 370 380 390 400
ESSIKLPKWP EAVERFQHSL QDKYKALPQS PRGPLVAVEL VRARLERGSN
410 420 430 440 450
KSQERELATP DWTERQLAHG GLAEVLQVVS DCRRPGETQV VAVLGKAGQG
460 470 480 490 500
KSHWARTVSH TWACGQLLQY DFVFYVPCHC LDRPGDTYHL RDLLCPPSLQ
510 520 530 540 550
PLAMDDEVLD YIVRQPDRVL LILDAFEELE AQDGLLHGPC GSLSPEPCSL
560 570 580 590 600
RGLLAGIFQR KLLRGCTLLL TARPRGRLAQ SLSKADAIFE VPSFSTKQAK
610 620 630 640 650
TYMRHYFENS GTAGNQDKAL GLLEGQPLLC SYSHSPVVCR AVCQLSKALL
660 670 680 690 700
EQGTEAQLPC TLTGLYVSLL GPAAQNSPPG ALVELAKLAW ELGRRHQSTL
710 720 730 740 750
QETRFSSVEV KTWAVTQGLM QQTLETTEAQ LAFSSFLLQC FLGAVWLAQC
760 770 780 790 800
NEIKDKELPQ YLALTPRKKR PYDNWLEGVP RFLAGLVFQP RAHCLGALVE
810 820 830 840 850
PAVAAVADRK QKVLTRYLKR LKLGTLRAGR LLELLHCAHE TQQPGIWEHV
860 870 880 890 900
AHQLPGHLSF LGTRLTPPDV YVLGRALETA SQDFSLDLRQ TGVEPSGLGN
910 920 930 940 950
LVGLSCVTSF RASLSDTMAL WESLQQQGEA QLLQAAEEKF TIEPFKAKSP
960 970 980 990 1000
KDVEDLDRLV QTQRLRNPSE DAAKDLPAIR DLKKLEFALG PILGPQAFPT
1010 1020 1030 1040 1050
LAKILPAFSS LQHLDLDSLS ENKIGDKGVS KLSATFPQLK ALETLNLSQN
1060 1070 1080 1090 1100
NITDVGACKL AEALPALAKS LLRLSLYNNC ICDKGAKSLA QVLPDMVSLR
1110 1120 1130 1140 1150
VMDVQFNKFT AAGAQQLASS LQKCPQVETL AMWTPTIPFG VQEHLQQLDA

RISLR
Length:1,155
Mass (Da):127,528
Last modified:April 23, 2003 - v2
Checksum:iF3FF05DEBFB0CE71
GO
Isoform 2 (identifier: P79621-2) [UniParc]FASTAAdd to basket

Also known as: III

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     78-94: SLQAPTVERGTSYRDHG → MRCLVPGPSGSYLPELQ

Show »
Length:1,078
Mass (Da):118,790
Checksum:i2BFEA61BBF581378
GO
Isoform 3 (identifier: P79621-3) [UniParc]FASTAAdd to basket

Also known as: IV

The sequence of this isoform differs from the canonical sequence as follows:
     1-101: Missing.

Show »
Length:1,054
Mass (Da):116,233
Checksum:iEE446E7796C6FED8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti994 – 9941G → R in AAB05004 (PubMed:8620527).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 101101Missing in isoform 3. 1 PublicationVSP_007216Add
BLAST
Alternative sequencei1 – 7777Missing in isoform 2. 2 PublicationsVSP_007214Add
BLAST
Alternative sequencei78 – 9417SLQAP…YRDHG → MRCLVPGPSGSYLPELQ in isoform 2. 2 PublicationsVSP_007215Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000006 Genomic DNA. Translation: AAB92364.2.
AF000007 Genomic DNA. Translation: AAB92365.1.
AF042158 mRNA. Translation: AAC34366.1.
AF042159 mRNA. Translation: AAC34367.1.
AF100709 mRNA. Translation: AAF06838.1.
AF100710 mRNA. Translation: AAF06839.1.
U60653 mRNA. Translation: AAB48859.1.
U46562 mRNA. Translation: AAB05004.1.
CCDSiCCDS27950.1. [P79621-2]
RefSeqiNP_001230690.1. NM_001243761.2.
NP_001289547.1. NM_001302618.1. [P79621-1]
NP_001289548.1. NM_001302619.1. [P79621-3]
NP_031601.1. NM_007575.4. [P79621-2]
XP_006521788.1. XM_006521725.2. [P79621-3]
XP_011244112.1. XM_011245810.1. [P79621-3]
UniGeneiMm.249560.
Mm.453401.
Mm.483581.

Genome annotation databases

EnsembliENSMUST00000023147; ENSMUSP00000023147; ENSMUSG00000022504. [P79621-2]
GeneIDi12265.
KEGGimmu:12265.
UCSCiuc007ydr.2. mouse. [P79621-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000006 Genomic DNA. Translation: AAB92364.2.
AF000007 Genomic DNA. Translation: AAB92365.1.
AF042158 mRNA. Translation: AAC34366.1.
AF042159 mRNA. Translation: AAC34367.1.
AF100709 mRNA. Translation: AAF06838.1.
AF100710 mRNA. Translation: AAF06839.1.
U60653 mRNA. Translation: AAB48859.1.
U46562 mRNA. Translation: AAB05004.1.
CCDSiCCDS27950.1. [P79621-2]
RefSeqiNP_001230690.1. NM_001243761.2.
NP_001289547.1. NM_001302618.1. [P79621-1]
NP_001289548.1. NM_001302619.1. [P79621-3]
NP_031601.1. NM_007575.4. [P79621-2]
XP_006521788.1. XM_006521725.2. [P79621-3]
XP_011244112.1. XM_011245810.1. [P79621-3]
UniGeneiMm.249560.
Mm.453401.
Mm.483581.

3D structure databases

ProteinModelPortaliP79621.
SMRiP79621. Positions 436-461, 973-1151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198417. 9 interactions.
STRINGi10090.ENSMUSP00000023147.

PTM databases

PhosphoSiteiP79621.

Proteomic databases

EPDiP79621.
PaxDbiP79621.
PRIDEiP79621.

Protocols and materials databases

DNASUi12265.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023147; ENSMUSP00000023147; ENSMUSG00000022504. [P79621-2]
GeneIDi12265.
KEGGimmu:12265.
UCSCiuc007ydr.2. mouse. [P79621-1]

Organism-specific databases

CTDi4261.
MGIiMGI:108445. Ciita.

Phylogenomic databases

eggNOGiKOG4308. Eukaryota.
ENOG410ZBX3. LUCA.
HOGENOMiHOG000107439.
HOVERGENiHBG000174.
InParanoidiP79621.
KOiK08060.
OMAiQKRPFPE.
OrthoDBiEOG7P5T07.
PhylomeDBiP79621.
TreeFamiTF352118.

Miscellaneous databases

PROiP79621.
SOURCEiSearch...

Gene expression databases

BgeeiP79621.
CleanExiMM_CIITA.
ExpressionAtlasiP79621. baseline and differential.
GenevisibleiP79621. MM.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR008095. MHC_II_transact.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 3 hits.
[Graphical view]
PRINTSiPR01719. MHCIIACTVATR.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of MHC class II molecules in different cellular and functional compartments is controlled by differential usage of multiple promoters of the transactivator CIITA."
    Muhlethaler-Mottet A., Otten L.A., Steimle V., Mach B.
    EMBO J. 16:2851-2860(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY, INDUCTION.
    Strain: BALB/cJ.
  2. "Mouse class II transactivator: cDNA sequence and amino acid comparison with the human class II transactivator."
    Sims T.N., Elliott J.F., Ramassar V., Denney D.W. Jr., Halloran P.F.
    Immunogenetics 45:220-222(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: NOD.
    Tissue: Spleen.
  3. "Interferon gamma (IFNgamma) gene transfer of an EMT6 tumor that is poorly responsive to IFNgamma stimulation: increase in tumor immunogenicity is accompanied by induction of a mouse class II transactivator and class II MHC."
    Panelli M.C., Wang E., Shen S., Schluter S.F., Bernstein R.M., Hersh E.M., Stopeck A., Gangavalli R., Barber J., Jolly D., Akporiaye E.T.
    Cancer Immunol. Immunother. 42:99-107(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 955-1097.
    Strain: BALB/cJ.
    Tissue: Thymus.

Entry informationi

Entry nameiC2TA_MOUSE
AccessioniPrimary (citable) accession number: P79621
Secondary accession number(s): O46787
, O78036, O78109, Q31115, Q9TPP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 23, 2003
Last modified: June 8, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.