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Protein

Neutral alpha-glucosidase AB

Gene

GANAB

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins.

Catalytic activityi

Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei542NucleophilePROSITE-ProRule annotation1
Active sitei545By similarity1
Active sitei618Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00957.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral alpha-glucosidase AB (EC:3.2.1.84)
Alternative name(s):
Alpha-glucosidase 2
Glucosidase II subunit alpha
Gene namesi
Name:GANAB
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32By similarityAdd BLAST32
ChainiPRO_000001857333 – 944Neutral alpha-glucosidase ABAdd BLAST912

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineBy similarity1
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Contains sialylated polysaccharide chains.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP79403.
PeptideAtlasiP79403.
PRIDEiP79403.

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000025647.

Structurei

3D structure databases

ProteinModelPortaliP79403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1066. Eukaryota.
COG1501. LUCA.
HOVERGENiHBG051683.
InParanoidiP79403.
KOiK05546.

Family and domain databases

InterProiIPR033403. DUF5110.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF17137. DUF5110. 1 hit.
PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 2 hits.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVAAVAAR RRRSWTGLVL ACLGVCLGLT LAVDRSNFKT CEESSFCKRQ
60 70 80 90 100
RSIRPGQSPY RALLDSLQLG PDTLTIHLIN EVTKVLLVLE LQGLQKNMTR
110 120 130 140 150
IRIDELEPRR PRYRVPDVLV AEPPTARLSV SGQDDNSVEV TVAEGPYKII
160 170 180 190 200
LTARPFRLDL LEDRSLLLSV NARGLLNFEH QRAPRVSQGS KDPAEGDGAQ
210 220 230 240 250
PEEAPGDGDK PEEIQGKAEK DEPGAWEETF KTHSDSKPYG PTSVGLDFSL
260 270 280 290 300
PGMEHVYGIP EHADSLRLKV TEGGDPYRLY NLDVFQYELY NPMALYGSVP
310 320 330 340 350
VLLAHSPHRD LGIFWLNAAE TWVDISSNTA GKTLFGKMLD YLQGSGETPQ
360 370 380 390 400
TDVRWMSESG IIDVFLLLGP SVFDVFRQYA SLTGTQALPP LFSLGYHQSR
410 420 430 440 450
WNYRDEADVL EVNQGFDDHN LPCDFIWLDI EHADGKRYFT WDPSRFPQPR
460 470 480 490 500
TMLEHLASKR RKLVAIVDPH IKVDSSYRVH EELQNLGLYV KTRDGSDYEG
510 520 530 540 550
WCWPGAASYP DFTNPKMRAW WADMFRFENY EGSSSNLYVW NDMNEPSVFN
560 570 580 590 600
GPEVTMLKDA QHYGGWEHRD LHNIYGFYVH MATADGLVLR SGGVERPFVL
610 620 630 640 650
SRAFFAGSQR FGAVWTGDNT AEWDHLKISI PMCLSLGLVG VSFCGADVGG
660 670 680 690 700
FFKNPEPELL VRWYQMGAYQ PFFRAHAHLD TGRREPWLLP TQYQDMIRDA
710 720 730 740 750
LGQRYSLLPF WYTLFYQAHR EGVPVMRALW VHYPQDVTTF SIDDEFLLGD
760 770 780 790 800
ALLVHPVTDS EAHGVQVYLP GQGEVWYDVH SYQKYHGPQT LYLPVTLSSI
810 820 830 840 850
PVFQRGGTIV PRWMRVRRSS DCMKDDPITL FVALSPQGTA QGELFLDDGH
860 870 880 890 900
TFNYQTGHEF LLRRFSFSGN TLVSSSADSK GHFETPVWIE RVVIIGAGKP
910 920 930 940
ATVVLQTKGS PESRLSFQHD PETSVLILRK PGVNVASDWS IHLR
Length:944
Mass (Da):106,662
Last modified:May 1, 1997 - v1
Checksum:iEB3096829037AAA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71273 mRNA. Translation: AAB49757.1.
RefSeqiNP_999069.1. NM_213904.1.
UniGeneiSsc.14543.

Genome annotation databases

GeneIDi396938.
KEGGissc:396938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71273 mRNA. Translation: AAB49757.1.
RefSeqiNP_999069.1. NM_213904.1.
UniGeneiSsc.14543.

3D structure databases

ProteinModelPortaliP79403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000025647.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Proteomic databases

PaxDbiP79403.
PeptideAtlasiP79403.
PRIDEiP79403.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396938.
KEGGissc:396938.

Organism-specific databases

CTDi23193.

Phylogenomic databases

eggNOGiKOG1066. Eukaryota.
COG1501. LUCA.
HOVERGENiHBG051683.
InParanoidiP79403.
KOiK05546.

Enzyme and pathway databases

UniPathwayiUPA00957.

Family and domain databases

InterProiIPR033403. DUF5110.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF17137. DUF5110. 1 hit.
PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 2 hits.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGANAB_PIG
AccessioniPrimary (citable) accession number: P79403
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 1997
Last modified: July 6, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.