Reviewed, UniProtKB/Swiss-Prot P79290 (NOS2_PIG)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II

Gene names

Name:

NOS2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	215 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Not stimulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Tissue specificity	Detected in both stimulated and unstimulated immune cells and macrophages with little or no up-regulation following cellular stimulation with lipopolysaccharides (LPS) or concanavalin A (ConA).
Induction	Little by LPS or ConA in spleen cells.
Sequence similarities	Belongs to the NOS family.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›215	›215	Nitric oxide synthase, inducible		PRO_0000170935
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	215	1

Sequences

	Sequence		Length	Mass (Da)
	P79290-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FECFF5BD85B65808	FASTA	215	24,716
	10 20 30 40 50 60 DARSCSTAQE MFEHICRHLR YATNNGNIRS AITVFPQRSD GKHDFRVWNA QLIRYAGYQM 70 80 90 100 110 120 PDGTIIGDPA SVEFTQLCID LGWKPKYGRF DVVPLVLHAD GRDPELFEIP PDLVLEVPME 130 140 150 160 170 180 HPKYEWFQEL ELKWYALPAV ANMLLEVGGL EFPGCPFNGW YMGTEIGVRD FCDVQRYNIL 190 200 210 EEVGRRMGLE THKLASLWKD RAVVEINVAV LHSFQ

References

[1]	"Inducible nitric oxide synthase expression in porcine immune cells." Pampusch M.S., Bennaars A.M., Harsch S., Murtaugh M.P. Vet. Immunol. Immunopathol. 61:279-289(1998) [PubMed: 9613441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U59390 mRNA. Translation: AAB40614.1.
UniGene	Ssc.16188
3D structure databases
HSSP	HSSP built from PDB template 4NOS based on UniProtKB P35228.
SMR	P79290. Positions 1-215.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P79290.
Family and domain databases
InterPro	IPR004030. NO_synthase_oxygenase_reg. [Graphical view]
Pfam	PF02898. NO_synthase. 1 hit. [Graphical view]
PROSITE	PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_PIG

Accession

Primary (citable) accession number: P79290

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)