P79290 (NOS2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitric oxide synthase, inducible
EC=1.14.13.39

Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2

Gene names

Name:

NOS2

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	215 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.
Catalytic activity	2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Not stimulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Tissue specificity	Detected in both stimulated and unstimulated immune cells and macrophages with little or no up-regulation following cellular stimulation with lipopolysaccharides (LPS) or concanavalin A (ConA).
Induction	Little by LPS or ConA in spleen cells.
Sequence similarities	Belongs to the NOS family.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro peptidyl-cysteine S-nitrosylation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›215	›215	Nitric oxide synthase, inducible		PRO_0000170935
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	215	1

Sequences

	Sequence		Length	Mass (Da)
	P79290 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: FECFF5BD85B65808	FASTA	215	24,716
	10 20 30 40 50 60 DARSCSTAQE MFEHICRHLR YATNNGNIRS AITVFPQRSD GKHDFRVWNA QLIRYAGYQM 70 80 90 100 110 120 PDGTIIGDPA SVEFTQLCID LGWKPKYGRF DVVPLVLHAD GRDPELFEIP PDLVLEVPME 130 140 150 160 170 180 HPKYEWFQEL ELKWYALPAV ANMLLEVGGL EFPGCPFNGW YMGTEIGVRD FCDVQRYNIL 190 200 210 EEVGRRMGLE THKLASLWKD RAVVEINVAV LHSFQ

References

[1]	"Inducible nitric oxide synthase expression in porcine immune cells." Pampusch M.S., Bennaars A.M., Harsch S., Murtaugh M.P. Vet. Immunol. Immunopathol. 61:279-289(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59390 mRNA. Translation: AAB40614.1.
UniGene	Ssc.16117.
3D structure databases
ProteinModelPortal	P79290.
SMR	P79290. Positions 1-215.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG4362.
HOGENOM	HOG000220884.
OrthoDB	EOG4D26P1.
Family and domain databases
InterPro	IPR004030. NO_synthase_oxygenase_dom. IPR026009. NOS. [Graphical view]
PANTHER	PTHR19384:SF5. PTHR19384:SF5. 1 hit.
Pfam	PF02898. NO_synthase. 1 hit. [Graphical view]
SUPFAM	SSF56512. NO_synthase_oxygenase_reg. 1 hit.
PROSITE	PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_PIG

Accession

Primary (citable) accession number: P79290

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program