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Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Not stimulated by calcium/calmodulin.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›215›215Nitric oxide synthase, induciblePRO_0000170935Add
BLAST

Expressioni

Tissue specificityi

Detected in both stimulated and unstimulated immune cells and macrophages with little or no up-regulation following cellular stimulation with lipopolysaccharides (LPS) or concanavalin A (ConA).

Inductioni

Little by LPS or ConA in spleen cells.

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 (By similarity). Interacts with GAPDH. Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000018812.

Structurei

3D structure databases

ProteinModelPortaliP79290.
SMRiP79290. Positions 1-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
InParanoidiP79290.

Family and domain databases

InterProiIPR004030. NOS_N.
[Graphical view]
PfamiPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.

Sequencei

Sequence statusi: Fragment.

P79290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DARSCSTAQE MFEHICRHLR YATNNGNIRS AITVFPQRSD GKHDFRVWNA
60 70 80 90 100
QLIRYAGYQM PDGTIIGDPA SVEFTQLCID LGWKPKYGRF DVVPLVLHAD
110 120 130 140 150
GRDPELFEIP PDLVLEVPME HPKYEWFQEL ELKWYALPAV ANMLLEVGGL
160 170 180 190 200
EFPGCPFNGW YMGTEIGVRD FCDVQRYNIL EEVGRRMGLE THKLASLWKD
210
RAVVEINVAV LHSFQ
Length:215
Mass (Da):24,716
Last modified:May 1, 1997 - v1
Checksum:iFECFF5BD85B65808
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei215 – 2151

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59390 mRNA. Translation: AAB40614.1.
UniGeneiSsc.16117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59390 mRNA. Translation: AAB40614.1.
UniGeneiSsc.16117.

3D structure databases

ProteinModelPortaliP79290.
SMRiP79290. Positions 1-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000018812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
InParanoidiP79290.

Family and domain databases

InterProiIPR004030. NOS_N.
[Graphical view]
PfamiPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Inducible nitric oxide synthase expression in porcine immune cells."
    Pampusch M.S., Bennaars A.M., Harsch S., Murtaugh M.P.
    Vet. Immunol. Immunopathol. 61:279-289(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiNOS2_PIG
AccessioniPrimary (citable) accession number: P79290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: June 24, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.