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P79290

- NOS2_PIG

UniProt

P79290 - NOS2_PIG

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

    Enzyme regulationi

    Not stimulated by calcium/calmodulin.By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro
    2. peptidyl-cysteine S-nitrosylation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, inducible (EC:1.14.13.39)
    Alternative name(s):
    Inducible NO synthase
    Short name:
    Inducible NOS
    Short name:
    iNOS
    NOS type II
    Peptidyl-cysteine S-nitrosylase NOS2
    Gene namesi
    Name:NOS2
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›215›215Nitric oxide synthase, induciblePRO_0000170935Add
    BLAST

    Expressioni

    Tissue specificityi

    Detected in both stimulated and unstimulated immune cells and macrophages with little or no up-regulation following cellular stimulation with lipopolysaccharides (LPS) or concanavalin A (ConA).

    Inductioni

    Little by LPS or ConA in spleen cells.

    Interactioni

    Subunit structurei

    Homodimer. Binds SLC9A3R1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP79290.
    SMRiP79290. Positions 1-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NOS family.Curated

    Phylogenomic databases

    eggNOGiCOG4362.
    HOGENOMiHOG000220884.

    Family and domain databases

    InterProiIPR004030. NOS_N.
    [Graphical view]
    PfamiPF02898. NO_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56512. SSF56512. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    P79290-1 [UniParc]FASTAAdd to Basket

    « Hide

    DARSCSTAQE MFEHICRHLR YATNNGNIRS AITVFPQRSD GKHDFRVWNA    50
    QLIRYAGYQM PDGTIIGDPA SVEFTQLCID LGWKPKYGRF DVVPLVLHAD 100
    GRDPELFEIP PDLVLEVPME HPKYEWFQEL ELKWYALPAV ANMLLEVGGL 150
    EFPGCPFNGW YMGTEIGVRD FCDVQRYNIL EEVGRRMGLE THKLASLWKD 200
    RAVVEINVAV LHSFQ 215
    Length:215
    Mass (Da):24,716
    Last modified:May 1, 1997 - v1
    Checksum:iFECFF5BD85B65808
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei215 – 2151

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59390 mRNA. Translation: AAB40614.1.
    UniGeneiSsc.16117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59390 mRNA. Translation: AAB40614.1 .
    UniGenei Ssc.16117.

    3D structure databases

    ProteinModelPortali P79290.
    SMRi P79290. Positions 1-215.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG4362.
    HOGENOMi HOG000220884.

    Family and domain databases

    InterProi IPR004030. NOS_N.
    [Graphical view ]
    Pfami PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56512. SSF56512. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Inducible nitric oxide synthase expression in porcine immune cells."
      Pampusch M.S., Bennaars A.M., Harsch S., Murtaugh M.P.
      Vet. Immunol. Immunopathol. 61:279-289(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.

    Entry informationi

    Entry nameiNOS2_PIG
    AccessioniPrimary (citable) accession number: P79290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3