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P79290

- NOS2_PIG

UniProt

P79290 - NOS2_PIG

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Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group.By similarity
Binds 1 FAD.By similarity
Binds 1 FMN.By similarity
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).By similarity

Enzyme regulationi

Not stimulated by calcium/calmodulin.By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›215›215Nitric oxide synthase, induciblePRO_0000170935Add
BLAST

Expressioni

Tissue specificityi

Detected in both stimulated and unstimulated immune cells and macrophages with little or no up-regulation following cellular stimulation with lipopolysaccharides (LPS) or concanavalin A (ConA).

Inductioni

Little by LPS or ConA in spleen cells.

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP79290.
SMRiP79290. Positions 1-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
InParanoidiP79290.

Family and domain databases

InterProiIPR004030. NOS_N.
[Graphical view]
PfamiPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.

Sequencei

Sequence statusi: Fragment.

P79290-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
DARSCSTAQE MFEHICRHLR YATNNGNIRS AITVFPQRSD GKHDFRVWNA
60 70 80 90 100
QLIRYAGYQM PDGTIIGDPA SVEFTQLCID LGWKPKYGRF DVVPLVLHAD
110 120 130 140 150
GRDPELFEIP PDLVLEVPME HPKYEWFQEL ELKWYALPAV ANMLLEVGGL
160 170 180 190 200
EFPGCPFNGW YMGTEIGVRD FCDVQRYNIL EEVGRRMGLE THKLASLWKD
210
RAVVEINVAV LHSFQ
Length:215
Mass (Da):24,716
Last modified:May 1, 1997 - v1
Checksum:iFECFF5BD85B65808
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei215 – 2151

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59390 mRNA. Translation: AAB40614.1.
UniGeneiSsc.16117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59390 mRNA. Translation: AAB40614.1 .
UniGenei Ssc.16117.

3D structure databases

ProteinModelPortali P79290.
SMRi P79290. Positions 1-215.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
InParanoidi P79290.

Family and domain databases

InterProi IPR004030. NOS_N.
[Graphical view ]
Pfami PF02898. NO_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF56512. SSF56512. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Inducible nitric oxide synthase expression in porcine immune cells."
    Pampusch M.S., Bennaars A.M., Harsch S., Murtaugh M.P.
    Vet. Immunol. Immunopathol. 61:279-289(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiNOS2_PIG
AccessioniPrimary (citable) accession number: P79290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3