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P79290 (NOS2_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:NOS2
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length215 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Not stimulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer. Binds SLC9A3R1 By similarity.

Tissue specificity

Detected in both stimulated and unstimulated immune cells and macrophages with little or no up-regulation following cellular stimulation with lipopolysaccharides (LPS) or concanavalin A (ConA).

Induction

Little by LPS or ConA in spleen cells.

Sequence similarities

Belongs to the NOS family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›215›215Nitric oxide synthase, inducible
PRO_0000170935

Experimental info

Non-terminal residue11
Non-terminal residue2151

Sequences

Sequence LengthMass (Da)Tools
P79290 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: FECFF5BD85B65808

FASTA21524,716
        10         20         30         40         50         60 
DARSCSTAQE MFEHICRHLR YATNNGNIRS AITVFPQRSD GKHDFRVWNA QLIRYAGYQM 

        70         80         90        100        110        120 
PDGTIIGDPA SVEFTQLCID LGWKPKYGRF DVVPLVLHAD GRDPELFEIP PDLVLEVPME 

       130        140        150        160        170        180 
HPKYEWFQEL ELKWYALPAV ANMLLEVGGL EFPGCPFNGW YMGTEIGVRD FCDVQRYNIL 

       190        200        210 
EEVGRRMGLE THKLASLWKD RAVVEINVAV LHSFQ 

« Hide

References

[1]"Inducible nitric oxide synthase expression in porcine immune cells."
Pampusch M.S., Bennaars A.M., Harsch S., Murtaugh M.P.
Vet. Immunol. Immunopathol. 61:279-289(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59390 mRNA. Translation: AAB40614.1.
UniGeneSsc.16117.

3D structure databases

ProteinModelPortalP79290.
SMRP79290. Positions 1-215.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG4362.
HOGENOMHOG000220884.

Family and domain databases

InterProIPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMSSF56512. SSF56512. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNOS2_PIG
AccessionPrimary (citable) accession number: P79290
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families