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Protein

Matrix metalloproteinase-20

Gene

MMP20

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
  • Ca2+By similarityNote: Binds 2 Calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100Zinc 1; in inhibited formBy similarity1
Metal bindingi164Calcium 1By similarity1
Metal bindingi165Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi166Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi176Zinc 2By similarity1
Metal bindingi178Zinc 2By similarity1
Metal bindingi183Calcium 2By similarity1
Metal bindingi184Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi186Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi188Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi191Zinc 2By similarity1
Metal bindingi197Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi198Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi200Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi202Calcium 1By similarity1
Metal bindingi204Zinc 2By similarity1
Metal bindingi206Calcium 2By similarity1
Metal bindingi209Calcium 2By similarity1
Metal bindingi226Zinc 1; catalyticBy similarity1
Active sitei227PROSITE-ProRule annotation1
Metal bindingi230Zinc 1; catalyticBy similarity1
Metal bindingi236Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B6. 6170.
ReactomeiR-SSC-1442490. Collagen degradation.

Protein family/group databases

MEROPSiM10.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-20 (EC:3.4.24.-)
Short name:
MMP-20
Alternative name(s):
Enamel metalloproteinase
Enamelysin
Gene namesi
Name:MMP20
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 9

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002883723 – 107Activation peptideSequence analysisAdd BLAST85
ChainiPRO_0000028838108 – 483Matrix metalloproteinase-20Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi296 ↔ 483By similarity

Post-translational modificationi

Autoactivates at least at the 107-Asn-|-Tyr-108 site.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

PaxDbiP79287.
PRIDEiP79287.

Expressioni

Tissue specificityi

Expressed specifically in the enamel organ.

Gene expression databases

GenevisibleiP79287. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015906.

Structurei

3D structure databases

ProteinModelPortaliP79287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati293 – 343Hemopexin 1Add BLAST51
Repeati344 – 389Hemopexin 2Add BLAST46
Repeati391 – 439Hemopexin 3Add BLAST49
Repeati440 – 483Hemopexin 4Add BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi98 – 105Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP79287.
KOiK07999.
OMAiYSYDERK.
OrthoDBiEOG091G03DP.
TreeFamiTF315428.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLPASGLA VLLVTALKFS AAAPSLFAAT PRTSRNNYHL AQAYLDKYYT
60 70 80 90 100
KKGGHQVGEM VAKGGNSMVK KIKELQAFFG LRVTGKLDRT TMDVIKRPRC
110 120 130 140 150
GVPDVANYRL FPGEPKWKKN TLTYRISKYT PSMTPAEVDK AMEMALQAWS
160 170 180 190 200
SAVPLSFVRV NAGEADIMIS FETGDHGDSY PFDGPRGTLA HAFAPGEGLG
210 220 230 240 250
GDTHFDNAEK WTMGMNGFNL FTVAAHEFGH ALGLAHSTDP SALMYPTYKY
260 270 280 290 300
QNPYGFHLPK DDVKGIQALY GPRKTFTGKP TVPHGPPHNP SLPDICDSSS
310 320 330 340 350
SFDAVTMLGK ELLFFRDRIF WRRQVHLMSG IRPSTITSSF PQLMSNVDAA
360 370 380 390 400
YEVADRGMAY FFKGPHYWIT RGFQMQGPPR TIYDFGFPRY VQRIDAAVHL
410 420 430 440 450
KDTQKTLFFV GDEYYSYDER KRKMDKDYPK NTEEEFSGVN GQIDAAVELN
460 470 480
GYIYFFSGPK AYKYDTEKED VVSVLKSNSW IGC
Length:483
Mass (Da):54,085
Last modified:May 1, 1997 - v1
Checksum:i1235DAAAEF1A20B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54825 mRNA. Translation: AAB41396.1.
PIRiJC5743.
RefSeqiNP_999070.1. NM_213905.1.
UniGeneiSsc.14550.

Genome annotation databases

EnsembliENSSSCT00000016345; ENSSSCP00000015906; ENSSSCG00000014983.
GeneIDi396939.
KEGGissc:396939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54825 mRNA. Translation: AAB41396.1.
PIRiJC5743.
RefSeqiNP_999070.1. NM_213905.1.
UniGeneiSsc.14550.

3D structure databases

ProteinModelPortaliP79287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015906.

Protein family/group databases

MEROPSiM10.019.

Proteomic databases

PaxDbiP79287.
PRIDEiP79287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000016345; ENSSSCP00000015906; ENSSSCG00000014983.
GeneIDi396939.
KEGGissc:396939.

Organism-specific databases

CTDi9313.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP79287.
KOiK07999.
OMAiYSYDERK.
OrthoDBiEOG091G03DP.
TreeFamiTF315428.

Enzyme and pathway databases

BRENDAi3.4.24.B6. 6170.
ReactomeiR-SSC-1442490. Collagen degradation.

Gene expression databases

GenevisibleiP79287. SS.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP20_PIG
AccessioniPrimary (citable) accession number: P79287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.