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Protein

Matrix metalloproteinase-20

Gene

MMP20

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
  • Ca2+By similarityNote: Binds 2 Calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi100 – 1001Zinc 1; in inhibited formBy similarity
Metal bindingi164 – 1641Calcium 1By similarity
Metal bindingi165 – 1651Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi166 – 1661Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi176 – 1761Zinc 2By similarity
Metal bindingi178 – 1781Zinc 2By similarity
Metal bindingi183 – 1831Calcium 2By similarity
Metal bindingi184 – 1841Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi186 – 1861Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi188 – 1881Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi191 – 1911Zinc 2By similarity
Metal bindingi197 – 1971Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi198 – 1981Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi200 – 2001Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi202 – 2021Calcium 1By similarity
Metal bindingi204 – 2041Zinc 2By similarity
Metal bindingi206 – 2061Calcium 2By similarity
Metal bindingi209 – 2091Calcium 2By similarity
Metal bindingi226 – 2261Zinc 1; catalyticBy similarity
Active sitei227 – 2271PROSITE-ProRule annotation
Metal bindingi230 – 2301Zinc 1; catalyticBy similarity
Metal bindingi236 – 2361Zinc 1; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B6. 6170.
ReactomeiR-SSC-1442490. Collagen degradation.

Protein family/group databases

MEROPSiM10.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-20 (EC:3.4.24.-)
Short name:
MMP-20
Alternative name(s):
Enamel metalloproteinase
Enamelysin
Gene namesi
Name:MMP20
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 9

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 10785Activation peptideSequence analysisPRO_0000028837Add
BLAST
Chaini108 – 483376Matrix metalloproteinase-20PRO_0000028838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi296 ↔ 483By similarity

Post-translational modificationi

Autoactivates at least at the 107-Asn-|-Tyr-108 site.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

PaxDbiP79287.

Expressioni

Tissue specificityi

Expressed specifically in the enamel organ.

Gene expression databases

GenevisibleiP79287. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015906.

Structurei

3D structure databases

ProteinModelPortaliP79287.
SMRiP79287. Positions 113-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati293 – 34351Hemopexin 1Add
BLAST
Repeati344 – 38946Hemopexin 2Add
BLAST
Repeati391 – 43949Hemopexin 3Add
BLAST
Repeati440 – 48344Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi98 – 1058Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP79287.
KOiK07999.
OMAiYSYDERK.
OrthoDBiEOG091G03DP.
TreeFamiTF315428.

Family and domain databases

CDDicd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P79287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLPASGLA VLLVTALKFS AAAPSLFAAT PRTSRNNYHL AQAYLDKYYT
60 70 80 90 100
KKGGHQVGEM VAKGGNSMVK KIKELQAFFG LRVTGKLDRT TMDVIKRPRC
110 120 130 140 150
GVPDVANYRL FPGEPKWKKN TLTYRISKYT PSMTPAEVDK AMEMALQAWS
160 170 180 190 200
SAVPLSFVRV NAGEADIMIS FETGDHGDSY PFDGPRGTLA HAFAPGEGLG
210 220 230 240 250
GDTHFDNAEK WTMGMNGFNL FTVAAHEFGH ALGLAHSTDP SALMYPTYKY
260 270 280 290 300
QNPYGFHLPK DDVKGIQALY GPRKTFTGKP TVPHGPPHNP SLPDICDSSS
310 320 330 340 350
SFDAVTMLGK ELLFFRDRIF WRRQVHLMSG IRPSTITSSF PQLMSNVDAA
360 370 380 390 400
YEVADRGMAY FFKGPHYWIT RGFQMQGPPR TIYDFGFPRY VQRIDAAVHL
410 420 430 440 450
KDTQKTLFFV GDEYYSYDER KRKMDKDYPK NTEEEFSGVN GQIDAAVELN
460 470 480
GYIYFFSGPK AYKYDTEKED VVSVLKSNSW IGC
Length:483
Mass (Da):54,085
Last modified:May 1, 1997 - v1
Checksum:i1235DAAAEF1A20B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54825 mRNA. Translation: AAB41396.1.
PIRiJC5743.
RefSeqiNP_999070.1. NM_213905.1.
UniGeneiSsc.14550.

Genome annotation databases

EnsembliENSSSCT00000016345; ENSSSCP00000015906; ENSSSCG00000014983.
GeneIDi396939.
KEGGissc:396939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54825 mRNA. Translation: AAB41396.1.
PIRiJC5743.
RefSeqiNP_999070.1. NM_213905.1.
UniGeneiSsc.14550.

3D structure databases

ProteinModelPortaliP79287.
SMRiP79287. Positions 113-272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015906.

Protein family/group databases

MEROPSiM10.019.

Proteomic databases

PaxDbiP79287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000016345; ENSSSCP00000015906; ENSSSCG00000014983.
GeneIDi396939.
KEGGissc:396939.

Organism-specific databases

CTDi9313.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP79287.
KOiK07999.
OMAiYSYDERK.
OrthoDBiEOG091G03DP.
TreeFamiTF315428.

Enzyme and pathway databases

BRENDAi3.4.24.B6. 6170.
ReactomeiR-SSC-1442490. Collagen degradation.

Gene expression databases

GenevisibleiP79287. SS.

Family and domain databases

CDDicd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP20_PIG
AccessioniPrimary (citable) accession number: P79287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.