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Protein

Alpha-(1,6)-fucosyltransferase

Gene

FUT8

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.1 Publication

Catalytic activityi

GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.

pH dependencei

Optimum pH is 7.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.68. 6170.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT23. Glycosyltransferase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-(1,6)-fucosyltransferase (EC:2.4.1.68)
Short name:
Alpha1-6FucT
Alternative name(s):
Fucosyltransferase 8
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
GDP-fucose--glycoprotein fucosyltransferase
Glycoprotein 6-alpha-L-fucosyltransferase
Gene namesi
Name:FUT8
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini31 – 575LumenalSequence analysisAdd BLAST545

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805291 – 575Alpha-(1,6)-fucosyltransferaseAdd BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi204 ↔ 266By similarity
Disulfide bondi212 ↔ 230By similarity
Disulfide bondi218 ↔ 222By similarity
Modified residuei278PhosphoserineBy similarity1
Disulfide bondi465 ↔ 472By similarity

Post-translational modificationi

Tyrosine phosphorylated by PKDCC/VLK.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP79282.
PRIDEiP79282.

Expressioni

Tissue specificityi

Highest expression in brain.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000002478.

Structurei

3D structure databases

ProteinModelPortaliP79282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 493GT23PROSITE-ProRule annotationAdd BLAST288
Domaini502 – 563SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 366Important for donor substrate bindingPROSITE-ProRule annotation2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi299 – 305SH3-bindingSequence analysis7

Sequence similaritiesi

Belongs to the glycosyltransferase 23 family.PROSITE-ProRule annotation
Contains 1 GT23 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, SH3-binding, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3705. Eukaryota.
ENOG410YHM8. LUCA.
HOGENOMiHOG000007175.
HOVERGENiHBG028260.
InParanoidiP79282.
KOiK00717.

Family and domain databases

InterProiIPR015827. Alpha1_6FUT_euk.
IPR027350. GT23_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51659. GT23. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P79282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHSDHSS RELSKILAKL
60 70 80 90 100
ERLKQQNEDL RRMAESLRIP EGPIDQGPAS GRVRALEEQF MKAKEQIENY
110 120 130 140 150
KKQTKNGPGK DHEILRRRIE NGAKELWFFL QSELKKLKNL EGNELQRHAD
160 170 180 190 200
EFLSDLGHHE RSIMTDLYYL SQTDGAGDWR EKEAKDLTEL VQRRITYLQN
210 220 230 240 250
PKDCSKAKKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT LALESHNWRY
260 270 280 290 300
ATGGWETVFR PVSETCTDRS GSSTGHWSGE VKDKNVQVVE LPIVDSVHPR
310 320 330 340 350
PPYLPLAVPE DLADRLVRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT
360 370 380 390 400
KKLGFKHPVI GVHVRRTDKV GAEAAFHPIE EYTVHVEEDF QLLARRMQVD
410 420 430 440 450
KKRVYLATDD PALLKEAKTK YPSYEFISDN SISWSAGLHN RYTENSLRGV
460 470 480 490 500
ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ ALHPDASANF RSLDDIYYFG
510 520 530 540 550
GPNAHNQIAI YPHQPRTEGE IPMEPGDIIG VAGNHWDGYP KGVNRKLGRT
560 570
GLYPSYKVRE KIETVKYPTY PEADK
Length:575
Mass (Da):66,229
Last modified:May 1, 1997 - v1
Checksum:i0F199D0BC2018F7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86723 mRNA. Translation: BAA13157.1.
RefSeqiNP_999064.1. NM_213899.1.
UniGeneiSsc.14532.

Genome annotation databases

GeneIDi396933.
KEGGissc:396933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86723 mRNA. Translation: BAA13157.1.
RefSeqiNP_999064.1. NM_213899.1.
UniGeneiSsc.14532.

3D structure databases

ProteinModelPortaliP79282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000002478.

Protein family/group databases

CAZyiGT23. Glycosyltransferase Family 23.

Proteomic databases

PaxDbiP79282.
PRIDEiP79282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396933.
KEGGissc:396933.

Organism-specific databases

CTDi2530.

Phylogenomic databases

eggNOGiKOG3705. Eukaryota.
ENOG410YHM8. LUCA.
HOGENOMiHOG000007175.
HOVERGENiHBG028260.
InParanoidiP79282.
KOiK00717.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.68. 6170.

Family and domain databases

InterProiIPR015827. Alpha1_6FUT_euk.
IPR027350. GT23_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51659. GT23. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUT8_PIG
AccessioniPrimary (citable) accession number: P79282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: May 1, 1997
Last modified: October 5, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.