Reviewed, UniProtKB/Swiss-Prot P79282 (FUT8_PIG)

Names and origin

Protein names

Recommended name:
    Alpha-(1,6)-fucosyltransferase
      Short name=alpha1-6FucT
    EC=2.4.1.68
Alternative name(s):
    Glycoprotein 6-alpha-L-fucosyltransferase
    GDP-fucose--glycoprotein fucosyltransferase
    GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
    Fucosyltransferase 8

Gene names

Name:

FUT8

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. Ref.1
Catalytic activity	GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Tissue specificity	Highest expression in brain.
Sequence similarities	Belongs to the glycosyltransferase 23 family. Contains 1 SH3 domain.
biophysicochemical properties	pH dependence: Optimum pH is 7.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	SH3 domain SH3-binding Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid glycosylation in Golgi Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW glycoprotein 6-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Alpha-(1,6)-fucosyltransferase		PRO_0000080529
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Signal-anchor for type II membrane protein Potential
	Topological domain	31 – 575	545	Lumenal Potential
	Domain	502 – 563	62	SH3
	Region	365 – 366	2	Important for donor substrate binding By similarity
	Motif	299 – 305	7	SH3-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P79282-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0F199D0BC2018F7B	FASTA	575	66,229
	10 20 30 40 50 60 MRPWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHSDHSS RELSKILAKL ERLKQQNEDL 70 80 90 100 110 120 RRMAESLRIP EGPIDQGPAS GRVRALEEQF MKAKEQIENY KKQTKNGPGK DHEILRRRIE 130 140 150 160 170 180 NGAKELWFFL QSELKKLKNL EGNELQRHAD EFLSDLGHHE RSIMTDLYYL SQTDGAGDWR 190 200 210 220 230 240 EKEAKDLTEL VQRRITYLQN PKDCSKAKKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT 250 260 270 280 290 300 LALESHNWRY ATGGWETVFR PVSETCTDRS GSSTGHWSGE VKDKNVQVVE LPIVDSVHPR 310 320 330 340 350 360 PPYLPLAVPE DLADRLVRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI 370 380 390 400 410 420 GVHVRRTDKV GAEAAFHPIE EYTVHVEEDF QLLARRMQVD KKRVYLATDD PALLKEAKTK 430 440 450 460 470 480 YPSYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ 490 500 510 520 530 540 ALHPDASANF RSLDDIYYFG GPNAHNQIAI YPHQPRTEGE IPMEPGDIIG VAGNHWDGYP 550 560 570 KGVNRKLGRT GLYPSYKVRE KIETVKYPTY PEADK

References

[1]

"Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-->6fucosyltransferase." Uozumi N., Yanagidani S., Miyoshi E., Ihara Y., Sakuma T., Gao C.-X., Teshima T., Fujii S., Shiba T., Taniguchi N. J. Biol. Chem. 271:27810-27817(1996) [PubMed: 8910378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 102-130; 333-344; 402-415 AND 566-575, FUNCTION. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86723 mRNA. Translation: BAA13157.1.
RefSeq	NP_999064.1.
UniGene	Ssc.14532
3D structure databases
SMR	P79282. Positions 109-572.
ModBase	Search...
Protein family/group databases
CAZy	GT23. Glycosyltransferase Family 23.
Genome annotation databases
GeneID	396933.
KEGG	ssc:396933.
Phylogenomic databases
HOVERGEN	P79282.
Enzyme and pathway databases
BRENDA	2.4.1.68. 249.
Family and domain databases
InterPro	IPR015827. Alpha1_6FUT_euk. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT8_PIG

Accession

Primary (citable) accession number: P79282

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)