P79266 (GASR_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrin/cholecystokinin type B receptor
Short name=CCK-B receptor
Short name=CCK-BR

Alternative name(s):
Cholecystokinin-2 receptor
Short name=CCK2-R

Gene names

Name:

CCKBR

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	elevation of cytosolic calcium ion concentration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	gastrin receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Gastrin/cholecystokinin type B receptor		PRO_0000069472
Regions
	Topological domain	1 – 57	57	Extracellular Potential
	Transmembrane	58 – 79	22	Helical; Name=1; Potential
	Topological domain	80 – 87	8	Cytoplasmic Potential
	Transmembrane	88 – 109	22	Helical; Name=2; Potential
	Topological domain	110 – 131	22	Extracellular Potential
	Transmembrane	132 – 150	19	Helical; Name=3; Potential
	Topological domain	151 – 170	20	Cytoplasmic Potential
	Transmembrane	171 – 189	19	Helical; Name=4; Potential
	Topological domain	190 – 219	30	Extracellular Potential
	Transmembrane	220 – 242	23	Helical; Name=5; Potential
	Topological domain	243 – 340	98	Cytoplasmic Potential
	Transmembrane	341 – 362	22	Helical; Name=6; Potential
	Topological domain	363 – 380	18	Extracellular Potential
	Transmembrane	381 – 401	21	Helical; Name=7; Potential
	Topological domain	402 – 454	53	Cytoplasmic Potential
Amino acid modifications
	Lipidation	415	1	S-palmitoyl cysteine By similarity
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Disulfide bond	127 ↔ 205		By similarity
Experimental info
	Sequence conflict	386	1	T → S in AAI12801. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P79266 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: A2846A580508ABA6	FASTA	454	48,781
	10 20 30 40 50 60 MELLKPNRSV LGSGPGPGAS LCRSGGPLLN GSGTGNLSCE PPRIRGAGTR ELELAIRVTL 70 80 90 100 110 120 YAVIFLMSVG GNVLIIVVLG LSRRLRTVTN AFLLSLAVSD LLLAVACMPF TLLPNLMGTF 130 140 150 160 170 180 IFGTVVCKAV SYFMGVSVSV STLSLVAIAL ERYSAICRPL QARVWQTRSH AARVIVATWM 190 200 210 220 230 240 LSGLLMVPYP VYTAVQPAGP RVLQCMHRWP SARVRQTWSV LLLLLLFFVP GVVMAVAYGL 250 260 270 280 290 300 ISRELYLGLR FDGDSDSESQ SRVGSQGGLP GGTGQGPAQA NGRCRSETRL AGEDGDGCYV 310 320 330 340 350 360 QLPRSRPALE MSALTAPTPG PGSGTRPAQA KLLAKKRVVR MLLVIVVLFF LCWLPVYSAN 370 380 390 400 410 420 TWRAFDGPGA HRALSGAPIS FIHLLTYASA CVNPLVYCFM HRRFRQACLD TCTRCCPRPP 430 440 450 RARPRPLPDE DPPTPSIASL SRLSYTTIST LGPG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, developmental expression and pharmacological characterization of the CCKB/gastrin receptor in the calf pancreas." Dufresne M., Escrieut C., Clerc P., le Huerou-Luron I., Prats H., Bertrand V., le Meuth V., Guilloteau P., Vaysse N., Fourmy D. Eur. J. Pharmacol. 297:165-179(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S83090 mRNA. Translation: AAB46896.1. BC112800 mRNA. Translation: AAI12801.1.
IPI	IPI00715431.
RefSeq	NP_776687.2. NM_174262.3.
UniGene	Bt.582.
3D structure databases
ProteinModelPortal	P79266.
SMR	P79266. Positions 359-386.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000006892.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000006892; ENSBTAP00000006892; ENSBTAG00000005236.
GeneID	281665.
KEGG	bta:281665.
Organism-specific databases
CTD	887.
Phylogenomic databases
eggNOG	NOG238659.
GeneTree	ENSGT00610000086012.
HOGENOM	HOG000294067.
HOVERGEN	HBG036927.
InParanoid	P79266.
KO	K04195.
OrthoDB	EOG43N7D1.
Family and domain databases
InterPro	IPR009126. Cholcskin_rcpt. IPR000314. Gastrin_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01822. CCYSTOKININR. PR00527. GASTRINR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P79266.
ChEMBL	CHEMBL2720.
NextBio	20805596.

Entry information

Entry name

GASR_BOVIN

Accession

Primary (citable) accession number: P79266
Secondary accession number(s): Q2KI20

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program