P79209 (NOS3_SHEEP) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 85. History...
Names and origin
|Protein names||Recommended name:|
Nitric oxide synthase, endothelial
NOS type III
|Organism||Ovis aries (Sheep) [Reference proteome]|
|Taxonomic identifier||9940 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis|
|Sequence length||99 AA.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity.
2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.
Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity.
Homodimer. Interacts with NOSIP and NOSTRIN By similarity.
Membrane › caveola By similarity. Cytoplasm › cytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity.
Belongs to the NOS family.
|Cellular component||Cell membrane|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||nitric oxide biosynthetic process|
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-SubCellcaveola
Inferred from electronic annotation. Source: UniProtKB-SubCellcytoskeleton
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Molecular_function||metal ion binding|
Inferred from electronic annotation. Source: UniProtKB-KWnitric-oxide synthase activity
Inferred from electronic annotation. Source: UniProtKB-EC
|Complete GO annotation...|
Sequence annotation (Features)
|||"Effect of hypoxia on the microvasculature of developing fetal brain of sheep: a studies on the expression pattern of constitutive forms of nitric oxide synthase."|
Aguan K., Weiner C.P.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Endothelial cell.
|U76738 mRNA. Translation: AAB40705.1.|
3D structure databases
|SMR||P79209. Positions 1-99. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR004030. NO_synthase_oxygenase_dom. |
|Pfam||PF02898. NO_synthase. 1 hit. |
|SUPFAM||SSF56512. SSF56512. 1 hit. |
|Accession||Primary (citable) accession number: P79209|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families