Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P79209

- NOS3_SHEEP

UniProt

P79209 - NOS3_SHEEP

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Nitric oxide synthase, endothelial

Gene

NOS3

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN (By similarity).By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, endothelial (EC:1.14.13.39)
Alternative name(s):
Constitutive NOS
Short name:
cNOS
EC-NOS
Endothelial NOS
Short name:
eNOS
NOS type III
Short name:
NOSIII
Gene namesi
Name:NOS3
Synonyms:ENOS
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Unplaced

Subcellular locationi

Membranecaveola By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity
Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity.By similarity

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›99›99Nitric oxide synthase, endothelialPRO_0000170947Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts with NOSIP and NOSTRIN (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP79209.
SMRiP79209. Positions 1-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NOS family.Curated

Family and domain databases

InterProiIPR004030. NOS_N.
[Graphical view]
PfamiPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.

Sequencei

Sequence statusi: Fragment.

P79209-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
VAVCMDLDTR TTSSLWKDKA AVEINLAVLH SFQLAKVTIV DHHAATVSFM
60 70 80 90
KHLENEQKAR GGCPADWAWI VPPISGSLTP VFHQEMVNYV LSPAFRYQP
Length:99
Mass (Da):11,034
Last modified:May 1, 1997 - v1
Checksum:i82C3C765557031DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei99 – 991

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76738 mRNA. Translation: AAB40705.1.
UniGeneiOar.521.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76738 mRNA. Translation: AAB40705.1 .
UniGenei Oar.521.

3D structure databases

ProteinModelPortali P79209.
SMRi P79209. Positions 1-99.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR004030. NOS_N.
[Graphical view ]
Pfami PF02898. NO_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF56512. SSF56512. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Effect of hypoxia on the microvasculature of developing fetal brain of sheep: a studies on the expression pattern of constitutive forms of nitric oxide synthase."
    Aguan K., Weiner C.P.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Endothelial cell.

Entry informationi

Entry nameiNOS3_SHEEP
AccessioniPrimary (citable) accession number: P79209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3