Reviewed,
UniProtKB/Swiss-Prot P79209 (NOS3_SHEEP)
Last modified
November 25, 2008.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nitric oxide synthase, endothelial EC=1.14.13.39 Alternative name(s): Endothelial NOS Short name=eNOS EC-NOS NOS type III Short name=NOSIII Constitutive NOS Short name=cNOS | ||||
| Gene names |
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| Organism | Ovis aries (Sheep) | ||||
| Taxonomic identifier | 9940 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis |
Protein attributes
| Sequence length | 99 AA. |
| Sequence status | Fragment. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity. |
| Catalytic activity | L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+). |
| Cofactor | Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. |
| Enzyme regulation | Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity. |
| Subunit structure | Homodimer. Interacts with NOSIP and NOSTRIN By similarity. |
| Subcellular location | Cell membrane › caveolaBy similarity. Cytoplasm › cytoskeletonBy similarity. Golgi apparatusBy similarity. Note= Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity. |
| Sequence similarities | Belongs to the NOS family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Cell membrane Cytoplasm Cytoskeleton Golgi apparatus Membrane |
| Ligand | Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW cytoskeletonInferred from electronic annotation. Source: UniProtKB-KW plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin bindingInferred from electronic annotation. Source: UniProtKB-KW iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
References
| [1] | "Effect of hypoxia on the microvasculature of developing fetal brain of sheep: a studies on the expression pattern of constitutive forms of nitric oxide synthase." Aguan K., Weiner C.P. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Endothelial cell. |
Cross-references
Sequence databases | |
|---|---|
| U76738 mRNA. Translation: AAB40705.1. | |
| UniGene | Oar.521 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1D0C based on UniProtKB P29473. |
| SMR | P79209. Positions 1-99. |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | P79209. |
Family and domain databases | |
| InterPro | IPR004030. NO_synthase_oxygenase_reg. [Graphical view] |
| Gene3D | G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit. |
| Pfam | PF02898. NO_synthase. 1 hit. [Graphical view] |
| PROSITE | PS60001. NOS. Partial match. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NOS3_SHEEP | ||||||||
| Accession | Primary (citable) accession number: P79209 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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