P79209 (NOS3_SHEEP) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 28, 2012.
Version 78.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nitric oxide synthase, endothelial EC=1.14.13.39 Alternative name(s): Constitutive NOS Short name=cNOS EC-NOS Endothelial NOS Short name=eNOS NOS type III Short name=NOSIII | ||||
| Gene names |
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| Organism | Ovis aries (Sheep) | ||||
| Taxonomic identifier | 9940 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis![]() |
Protein attributes
| Sequence length | 99 AA. |
| Sequence status | Fragment. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity. |
| Catalytic activity | 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. |
| Cofactor | Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. |
| Enzyme regulation | Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity. |
| Subunit structure | Homodimer. Interacts with NOSIP and NOSTRIN By similarity. |
| Subcellular location | Membrane › caveola By similarity. Cytoplasm › cytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity. |
| Sequence similarities | Belongs to the NOS family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Cytoplasm Cytoskeleton Golgi apparatus Membrane |
| Ligand | Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological_process | nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Cellular_component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell caveolaInferred from electronic annotation. Source: UniProtKB-SubCell cytoskeletonInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
References
| [1] | "Effect of hypoxia on the microvasculature of developing fetal brain of sheep: a studies on the expression pattern of constitutive forms of nitric oxide synthase." Aguan K., Weiner C.P. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Endothelial cell. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U76738 mRNA. Translation: AAB40705.1. |
| UniGene | Oar.521. |
3D structure databases | |
| ProteinModelPortal | P79209. |
| SMR | P79209. Positions 1-99. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR004030. NO_synthase_oxygenase_dom. IPR026009. NOS. [Graphical view] |
| PANTHER | PTHR19384:SF5. PTHR19384:SF5. 1 hit. |
| Pfam | PF02898. NO_synthase. 1 hit. [Graphical view] |
| SUPFAM | SSF56512. NO_synthase_oxygenase_reg. 1 hit. |
| PROSITE | PS60001. NOS. Partial match. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NOS3_SHEEP | ||||||||
| Accession | Primary (citable) accession number: P79209 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with
