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P79209 (NOS3_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, endothelial

EC=1.14.13.39
Alternative name(s):
Constitutive NOS
Short name=cNOS
EC-NOS
Endothelial NOS
Short name=eNOS
NOS type III
Short name=NOSIII
Gene names
Name:NOS3
Synonyms:ENOS
OrganismOvis aries (Sheep) [Complete proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length99 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity.

Subunit structure

Homodimer. Interacts with NOSIP and NOSTRIN By similarity.

Subcellular location

Membranecaveola By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity.

Sequence similarities

Belongs to the NOS family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›99›99Nitric oxide synthase, endothelial
PRO_0000170947

Experimental info

Non-terminal residue11
Non-terminal residue991

Sequences

Sequence LengthMass (Da)Tools
P79209 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 82C3C765557031DA

FASTA9911,034
        10         20         30         40         50         60 
VAVCMDLDTR TTSSLWKDKA AVEINLAVLH SFQLAKVTIV DHHAATVSFM KHLENEQKAR 

        70         80         90 
GGCPADWAWI VPPISGSLTP VFHQEMVNYV LSPAFRYQP 

« Hide

References

[1]"Effect of hypoxia on the microvasculature of developing fetal brain of sheep: a studies on the expression pattern of constitutive forms of nitric oxide synthase."
Aguan K., Weiner C.P.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Endothelial cell.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76738 mRNA. Translation: AAB40705.1.
UniGeneOar.521.

3D structure databases

ProteinModelPortalP79209.
SMRP79209. Positions 1-99.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMSSF56512. SSF56512. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNOS3_SHEEP
AccessionPrimary (citable) accession number: P79209
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families