Reviewed, UniProtKB/Swiss-Prot P79202 (CP11A_SHEEP)

Names and origin

Protein names

Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    Cytochrome P450 11A1
    CYPXIA1
    Cytochrome P450(scc)
    Cholesterol desmolase

Gene names

Name:

CYP11A1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Catalytic activity	Cholesterol + reduced adrenal ferredoxin + O2 = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol monooxygenase (side-chain-cleaving) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion Ref.2
	Chain	40 – 520	481	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000003591
Sites
	Metal binding	461	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P79202-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: DF8989FB8A87E8AE	FASTA	520	60,357
	10 20 30 40 50 60 MLARGLPFRS ALVKACPPLL NTGREGWGHH RVGTGEGAGI STRTPRPYSE IPSPGDNGWI 70 80 90 100 110 120 NLYHFWRKKG SQRIHFHHIE NFQKYGPIYR EKLGNLESVY IIHPEGVAHL FKFEGSYPQR 130 140 150 160 170 180 YDIPPWLAYH RYYQKPIGVL FKKSGAWKKD RVVLNTEVMA PEAIKNFIPL LNPVSQDFVS 190 200 210 220 230 240 LLHKRIKQQG SGKFVGDIKE DLFRFAFESI TNVMFGERLG MLEDTVDTEA QKFIDAVYKM 250 260 270 280 290 300 FHTSVPLLNL PPELYRLFRT KTWRDHVAAW DTIFNKAEKY TEIFYQDLRQ KTEFRNYPGI 310 320 330 340 350 360 LYHLLKSEKM LLEDVKANIT EMLAGGVDTT SMTLQWHLYE MARSLNVQEM LRKEVLNARR 370 380 390 400 410 420 QAEGDISKML QMVPLLKASI KETLRLHPIS VTLQRYPESD LVLQDYLIPA KTLVQVAIYA 430 440 450 460 470 480 MGRDPAFFSN PDKFDPTRWL GKDKDLIHFR NLGFGWGVRQ CVGRRIAELE MTLFLIHILE 490 500 510 520 NFRVEMQQIG DVNTIFNLIL TPDKPIFLVF RPFNQGPPQA

References

[1]	"Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450scc (CYP11A1)." Okuyama E., Okazaki T., Furukawa A., Wu R.-F., Ichikawa Y. J. Steroid Biochem. Mol. Biol. 57:179-185(1996) [PubMed: 8645627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal cortex.
[2]	"Purification and comparative characterization of cytochrome P-450scc (CYP XIA1) from sheep adrenocortical mitochondria." Miyatake A., Tsubaki M., Hori H., Ichikawa Y. Biochim. Biophys. Acta 1215:176-182(1994) [PubMed: 7948001] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-62. Tissue: Adrenal cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50057 mRNA. Translation: BAA08775.1.
RefSeq	NP_001087258.1.
UniGene	Oar.5829
3D structure databases
SMR	P79202. Positions 43-514.
ModBase	Search...
Genome annotation databases
GeneID	100048994.
Organism-specific databases
CTD	100048994.
Phylogenomic databases
HOVERGEN	P79202.
Enzyme and pathway databases
BRENDA	1.14.15.6. 271.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP11A_SHEEP

Accession

Primary (citable) accession number: P79202

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)