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P79143

- AMPN_CANFA

UniProt

P79143 - AMPN_CANFA

Protein

Aminopeptidase N

Gene

ANPEP

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis By similarity. May serve as a receptor for bovine coronavirus (BCV) in a species-specific manner.By similarity

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. virus receptor activity Source: UniProtKB-KW

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. cell differentiation Source: UniProtKB-KW
    3. viral process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aminopeptidase, Developmental protein, Host cell receptor for virus entry, Hydrolase, Metalloprotease, Protease, Receptor

    Keywords - Biological processi

    Angiogenesis, Differentiation, Host-virus interaction

    Keywords - Ligandi

    Zinc

    Enzyme and pathway databases

    ReactomeiREACT_204976. Metabolism of Angiotensinogen to Angiotensins.

    Protein family/group databases

    MEROPSiM01.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Short name:
    AP-N
    Short name:
    cAPN
    Alternative name(s):
    Alanyl aminopeptidase
    Aminopeptidase M
    Short name:
    AP-M
    Microsomal aminopeptidase
    CD_antigen: CD13
    Gene namesi
    Name:ANPEP
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment Source: Ensembl
    2. external side of plasma membrane Source: Ensembl
    3. lysosomal membrane Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›191›191Aminopeptidase NPRO_0000095079Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi113 ↔ 120By similarity
    Disulfide bondi150 ↔ 186By similarity
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer By similarity. Interacts with CCoV spike glycoprotein.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000017677.

    Structurei

    3D structure databases

    ProteinModelPortaliP79143.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni‹1 – ›191›191MetalloproteaseAdd
    BLAST

    Domaini

    Amino acids 1-191 are essential to mediate susceptibility to infection with CCV (in canine/human chimeric studies). This region can confer susceptibility to infection with TGEV and FIPV (strain 79-1146) when substituted in corresponding human ANPEP region.

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0308.
    HOGENOMiHOG000106482.
    InParanoidiP79143.

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P79143-1 [UniParc]FASTAAdd to Basket

    « Hide

    DLSVIPVINR AQVIHDTFDL ASAQIVPVTL ALNSTLFLNQ ETEYMPWEAA    50
    LSSLSYFKLM FDRSEVYGPM KNYLRKQVTP LFNHFEKITQ NWTDHPQTLT 100
    EQYNEINAVS TACTYGVPKC KDLVSTLFAE WRKNPQNNPI YPNLRSTVYC 150
    NAIAQGGEEE WNFVWEQFRN TSLVNEADKL RSALACSTQV W 191
    Length:191
    Mass (Da):21,994
    Last modified:May 1, 1997 - v1
    Checksum:i43C2378CAC657D8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei191 – 1911

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98239 mRNA. Translation: CAA66895.1.
    UniGeneiCfa.3774.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98239 mRNA. Translation: CAA66895.1 .
    UniGenei Cfa.3774.

    3D structure databases

    ProteinModelPortali P79143.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000017677.

    Protein family/group databases

    MEROPSi M01.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0308.
    HOGENOMi HOG000106482.
    InParanoidi P79143.

    Enzyme and pathway databases

    Reactomei REACT_204976. Metabolism of Angiotensinogen to Angiotensins.

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus."
      Benbacer L., Kut E., Besnardeau L., Laude H., Delmas B.
      J. Virol. 71:734-737(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CCOV SPIKE GLYCOPROTEIN, IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR CCOV INFECTION.
      Tissue: Small intestine.

    Entry informationi

    Entry nameiAMPN_CANFA
    AccessioniPrimary (citable) accession number: P79143
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 23, 2003
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3