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Protein

Aminopeptidase N

Gene

ANPEP

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines and involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis (By similarity). May serve as a receptor for bovine coronavirus (BCV) in a species-specific manner.By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. metallopeptidase activity Source: UniProtKB-KW
  3. virus receptor activity Source: UniProtKB-KW

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. cell differentiation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Developmental protein, Host cell receptor for virus entry, Hydrolase, Metalloprotease, Protease, Receptor

Keywords - Biological processi

Angiogenesis, Differentiation, Host-virus interaction

Keywords - Ligandi

Zinc

Enzyme and pathway databases

ReactomeiREACT_204976. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiM01.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase N (EC:3.4.11.2)
Short name:
AP-N
Short name:
cAPN
Alternative name(s):
Alanyl aminopeptidase
Aminopeptidase M
Short name:
AP-M
Microsomal aminopeptidase
CD_antigen: CD13
Gene namesi
Name:ANPEP
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum-Golgi intermediate compartment Source: Ensembl
  2. external side of plasma membrane Source: Ensembl
  3. extracellular space Source: Ensembl
  4. extracellular vesicular exosome Source: Ensembl
  5. lysosomal membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›191›191Aminopeptidase NPRO_0000095079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi113 ↔ 120By similarity
Disulfide bondi150 ↔ 186By similarity
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CCoV spike glycoprotein.By similarity1 Publication

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000017677.

Structurei

3D structure databases

ProteinModelPortaliP79143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni‹1 – ›191›191MetalloproteaseAdd
BLAST

Domaini

Amino acids 1-191 are essential to mediate susceptibility to infection with CCV (in canine/human chimeric studies). This region can confer susceptibility to infection with TGEV and FIPV (strain 79-1146) when substituted in corresponding human ANPEP region.

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiCOG0308.
HOGENOMiHOG000106482.
InParanoidiP79143.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P79143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DLSVIPVINR AQVIHDTFDL ASAQIVPVTL ALNSTLFLNQ ETEYMPWEAA
60 70 80 90 100
LSSLSYFKLM FDRSEVYGPM KNYLRKQVTP LFNHFEKITQ NWTDHPQTLT
110 120 130 140 150
EQYNEINAVS TACTYGVPKC KDLVSTLFAE WRKNPQNNPI YPNLRSTVYC
160 170 180 190
NAIAQGGEEE WNFVWEQFRN TSLVNEADKL RSALACSTQV W
Length:191
Mass (Da):21,994
Last modified:May 1, 1997 - v1
Checksum:i43C2378CAC657D8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei191 – 1911

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98239 mRNA. Translation: CAA66895.1.
UniGeneiCfa.3774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98239 mRNA. Translation: CAA66895.1.
UniGeneiCfa.3774.

3D structure databases

ProteinModelPortaliP79143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000017677.

Protein family/group databases

MEROPSiM01.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG0308.
HOGENOMiHOG000106482.
InParanoidiP79143.

Enzyme and pathway databases

ReactomeiREACT_204976. Metabolism of Angiotensinogen to Angiotensins.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus."
    Benbacer L., Kut E., Besnardeau L., Laude H., Delmas B.
    J. Virol. 71:734-737(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CCOV SPIKE GLYCOPROTEIN, IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR CCOV INFECTION.
    Tissue: Small intestine.

Entry informationi

Entry nameiAMPN_CANFA
AccessioniPrimary (citable) accession number: P79143
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.